Phospholipid homeostasis maintains cell polarity,development and virulence in Metarhiziumrobertsii
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- 英文论文题名：Phospholipid homeostasis maintains cell polarity,development and virulence in Metarhiziumrobertsii
- 论文作者：Qiang Gao ; Yuzhen Lu ; Hongyan Yao ; Yong-Jiang Xu ; Wei Huang ; Chengshu Wang
- 英文论文作者：Qiang Gao ; Yuzhen Lu ; Hongyan Yao ; Yong-Jiang Xu ; Wei Huang ; Chengshu Wang ; Key Laboratory of Insect Developmental and Evolutionary Biology ; Institute of Plant Physiology and Ecology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences
- 年：2016
- 作者机构：Key Laboratory of Insect Developmental and Evolutionary Biology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences;
- 英文论文关键词：glycerol phosphate pathway ; phospholipid ; lysophosphatidateacyltransferase ; Metarhiziumrobertsii
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：S476.12
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：3
- 文件大小：379k
- 原文格式：D
- 会议级别：全国
摘要
The final product of the glycerol phosphate(GP) pathway is triacylglycerol(TAG) that regulates the homeostasis of energy,fatty acids and phospholipids in cells.The enzymes involved in this pathway have been characterized in many model organisms;however,their contributions to fungal infection are largely unclear.In this study,we performed serial deletion of genes in the GP pathway in the insect pathogenic fungus Metarhiziumrobertsii.The results indicated that a lysophosphatidateacyltransferasemr LPAAT1 was required for fungal growth,cell differentiation,maintenance of cell polarity and virulence.Lipidomic analysis indicated that deletion of mr LPAAT1 resulted in significant increases in TAG,fatty acids and phosphatidylcholine(PC) but decreased phosphatidic acid(PA),phosphatidylethanolamine(PE) and other species of phospholipids when compared to the wild type.Disruption of the isozymatic gene mr LPAAT2,however,resulted in a reduction in PC but not PA in the mutant cells.There were no changes in development and virulence in ?mr LPAAT2.Phospholipid feeding assays verified that a PE supplement could rescue the cell differentiation defect in ?mr LPAAT1.The results of this study reveal that cellular phospholipid homeostasis mediated by the GP pathway regulates fungal growth,cell polarity,differentiation and virulence.
The final product of the glycerol phosphate(GP) pathway is triacylglycerol(TAG) that regulates the homeostasis of energy,fatty acids and phospholipids in cells.The enzymes involved in this pathway have been characterized in many model organisms;however,their contributions to fungal infection are largely unclear.In this study,we performed serial deletion of genes in the GP pathway in the insect pathogenic fungus Metarhiziumrobertsii.The results indicated that a lysophosphatidateacyltransferasemr LPAAT1 was required for fungal growth,cell differentiation,maintenance of cell polarity and virulence.Lipidomic analysis indicated that deletion of mr LPAAT1 resulted in significant increases in TAG,fatty acids and phosphatidylcholine(PC) but decreased phosphatidic acid(PA),phosphatidylethanolamine(PE) and other species of phospholipids when compared to the wild type.Disruption of the isozymatic gene mr LPAAT2,however,resulted in a reduction in PC but not PA in the mutant cells.There were no changes in development and virulence in ?mr LPAAT2.Phospholipid feeding assays verified that a PE supplement could rescue the cell differentiation defect in ?mr LPAAT1.The results of this study reveal that cellular phospholipid homeostasis mediated by the GP pathway regulates fungal growth,cell polarity,differentiation and virulence.
The final product of the glycerol phosphate(GP) pathway is triacylglycerol(TAG) that regulates the homeostasis of energy,fatty acids and phospholipids in cells.The enzymes involved in this pathway have been characterized in many model organisms;however,their contributions to fungal infection are largely unclear.In this study,we performed serial deletion of genes in the GP pathway in the insect pathogenic fungus Metarhiziumrobertsii.The results indicated that a lysophosphatidateacyltransferasemr LPAAT1 was required for fungal growth,cell differentiation,maintenance of cell polarity and virulence.Lipidomic analysis indicated that deletion of mr LPAAT1 resulted in significant increases in TAG,fatty acids and phosphatidylcholine(PC) but decreased phosphatidic acid(PA),phosphatidylethanolamine(PE) and other species of phospholipids when compared to the wild type.Disruption of the isozymatic gene mr LPAAT2,however,resulted in a reduction in PC but not PA in the mutant cells.There were no changes in development and virulence in ?mr LPAAT2.Phospholipid feeding assays verified that a PE supplement could rescue the cell differentiation defect in ?mr LPAAT1.The results of this study reveal that cellular phospholipid homeostasis mediated by the GP pathway regulates fungal growth,cell polarity,differentiation and virulence.
引文
Benghezal M,Roubaty C,Veepuri V,Knudsen J,Conzelmann A(2007)SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast.The Journal of biological chemistry 282:30845-30855
Carman GM,Henry SA(2007)Phosphatidic acid plays a central role in the transcriptional regulation of glycerophospholipid synthesis in Saccharomyces cerevisiae.The Journal of biological chemistry282:37293-37297
Coleman RA,Lee DP(2004)Enzymes of triacylglycerol synthesis and their regulation.Prog Lipid Res43:134-176
Connerth M,Tatsuta T,Haag M,Klecker T,Westermann B,Langer T(2012)Intramitochondrial Transport of Phosphatidic Acid in Yeast by a Lipid Transfer Protein.Science 338:815-818
Csaki LS,Dwyer JR,Fong LG,Tontonoz P,Young SG,Reue K(2013)Lipins,lipinopathies,and the modulation of cellular lipid storage and signaling.Prog Lipid Res 52:305-316
Dagdas YF,Yoshino K,Dagdas G,Ryder LS,Bielska E,Steinberg G,Talbot NJ(2012)Septin-Mediated Plant Cell Invasion by the Rice Blast Fungus,Magnaporthe oryzae.Science 336:1590-1595
Fairn GD,Hermansson M,Somerharju P,Grinstein S(2011)Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity.Nature cell biology13:1424-U1498
Gao Q,Shang Y,Huang W,Wang C(2013)Glycerol-3-phosphate Acyltransferase contributes to triacylglycerol biosynthesis,lipid droplet formation,and host invasion in Metarhizium robertsii.Applied and environmental microbiology 79:7646-7653
Hamai C,Yang TL,Kataoka S,Cremer PS,Musser SM(2006)Effect of average phospholipid curvature on supported bilayer formation on glass by vesicle fusion.Biophys J 90:1241-1248
Harris SD(2011)Cdc42/Rho GTPases in fungi:variations on a common theme.Molecular microbiology79:1123-1127
Henry SA,Kohlwein SD,Carman GM(2012)Metabolism and regulation of glycerolipids in the yeast Saccharomyces cerevisiae.Genetics 190:317-349
Pascual F,Soto-Cardalda A,Carman GM(2013)PAH1-encoded Phosphatidate Phosphatase Plays a Role in the Growth Phase-and Inositol-mediated Regulation of Lipid Synthesis in Saccharomyces cerevisiae.Journal of Biological Chemistry 288:35781-35792
Riekhof WR,Wu J,Jones JL,Voelker DR(2007)Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae.Journal of Biological Chemistry 282:28344-28352
Scheffer J,Chen CB,Heidrich P,Dickman MB,Tudzynski P(2005)A CDC42 homologue in Claviceps purpurea is involved in vegetative differentiation and is essential for pathogenicity.Eukaryotic cell4:1228-1238
Shindou H,Hishikawa D,Harayama T,Yuki K,Shimizu T(2009)Recent progress on acyl Co A:lysophospholipid acyltransferase research.J Lipid Res 50 Suppl:S46-51
Si H,Justa-Schuch D,Seiler S,Harris SD(2010)Regulation of septum formation by the Bud3-Rho4GTPase module in Aspergillus nidulans.Genetics 185:165-176
St Leger RJ,Wang CS(2010)Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests.Applied microbiology and biotechnology 85:901-907
Suetsugu S,Kurisu S,Takenawa T(2014)Dynamic shaping of cellular membranes by phospholipids and membrane-deforming proteins.Physiological reviews 94:1219-1248
Takeuchi K,Reue K(2009)Biochemistry,physiology,and genetics of GPAT,AGPAT,and lipin enzymes in triglyceride synthesis.Am J Physiol-Endoc M 296:E1195-E1209
Tamaki H,Shimada A,Ito Y,Ohya M,Takase J,Miyashita M,Miyagawa H,Nozaki H,Nakayama R,Kumagai H(2007)LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.Journal of Biological Chemistry282:34288-34298
Thines E,Weber RW,Talbot NJ(2000)MAP kinase and protein kinase A-dependent mobilization of triacylglycerol and glycogen during appressorium turgor generation by Magnaporthe grisea.The Plant cell 12:1703-1718
Veneault-Fourrey C,Lambou K,Lebrun MH(2006)Fungal Pls1 tetraspanins as key factors of penetration into host plants:a role in re-establishing polarized growth in the appressorium?Fems Microbiol Lett 256:179-184
Virag A,Lee MP,Si HY,Harris SD(2007)Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans.Molecular microbiology 66:1579-1596
Wang CS,Leger RJS(2007)The Metarhizium anisopliae perilipin homolog MPL1 regulates lipid metabolism,appressorial turgor pressure,and virulence.Journal of Biological Chemistry282:21110-21115
Xu YJ,Luo F,Gao Q,Shang Y,Wang C(2015)Metabolomics reveals insect metabolic responses associated with fungal infection.Analytical and bioanalytical chemistry
Zheng W,Zhao Z,Chen J,Liu W,Ke H,Zhou J,Lu G,Darvill AG,Albersheim P,Wu S,Wang Z(2009)A Cdc42 ortholog is required for penetration and virulence of Magnaporthe grisea.Fungal Genetics and Biology 46:450-460
Carman GM,Henry SA(2007)Phosphatidic acid plays a central role in the transcriptional regulation of glycerophospholipid synthesis in Saccharomyces cerevisiae.The Journal of biological chemistry282:37293-37297
Coleman RA,Lee DP(2004)Enzymes of triacylglycerol synthesis and their regulation.Prog Lipid Res43:134-176
Connerth M,Tatsuta T,Haag M,Klecker T,Westermann B,Langer T(2012)Intramitochondrial Transport of Phosphatidic Acid in Yeast by a Lipid Transfer Protein.Science 338:815-818
Csaki LS,Dwyer JR,Fong LG,Tontonoz P,Young SG,Reue K(2013)Lipins,lipinopathies,and the modulation of cellular lipid storage and signaling.Prog Lipid Res 52:305-316
Dagdas YF,Yoshino K,Dagdas G,Ryder LS,Bielska E,Steinberg G,Talbot NJ(2012)Septin-Mediated Plant Cell Invasion by the Rice Blast Fungus,Magnaporthe oryzae.Science 336:1590-1595
Fairn GD,Hermansson M,Somerharju P,Grinstein S(2011)Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity.Nature cell biology13:1424-U1498
Gao Q,Shang Y,Huang W,Wang C(2013)Glycerol-3-phosphate Acyltransferase contributes to triacylglycerol biosynthesis,lipid droplet formation,and host invasion in Metarhizium robertsii.Applied and environmental microbiology 79:7646-7653
Hamai C,Yang TL,Kataoka S,Cremer PS,Musser SM(2006)Effect of average phospholipid curvature on supported bilayer formation on glass by vesicle fusion.Biophys J 90:1241-1248
Harris SD(2011)Cdc42/Rho GTPases in fungi:variations on a common theme.Molecular microbiology79:1123-1127
Henry SA,Kohlwein SD,Carman GM(2012)Metabolism and regulation of glycerolipids in the yeast Saccharomyces cerevisiae.Genetics 190:317-349
Pascual F,Soto-Cardalda A,Carman GM(2013)PAH1-encoded Phosphatidate Phosphatase Plays a Role in the Growth Phase-and Inositol-mediated Regulation of Lipid Synthesis in Saccharomyces cerevisiae.Journal of Biological Chemistry 288:35781-35792
Riekhof WR,Wu J,Jones JL,Voelker DR(2007)Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae.Journal of Biological Chemistry 282:28344-28352
Scheffer J,Chen CB,Heidrich P,Dickman MB,Tudzynski P(2005)A CDC42 homologue in Claviceps purpurea is involved in vegetative differentiation and is essential for pathogenicity.Eukaryotic cell4:1228-1238
Shindou H,Hishikawa D,Harayama T,Yuki K,Shimizu T(2009)Recent progress on acyl Co A:lysophospholipid acyltransferase research.J Lipid Res 50 Suppl:S46-51
Si H,Justa-Schuch D,Seiler S,Harris SD(2010)Regulation of septum formation by the Bud3-Rho4GTPase module in Aspergillus nidulans.Genetics 185:165-176
St Leger RJ,Wang CS(2010)Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests.Applied microbiology and biotechnology 85:901-907
Suetsugu S,Kurisu S,Takenawa T(2014)Dynamic shaping of cellular membranes by phospholipids and membrane-deforming proteins.Physiological reviews 94:1219-1248
Takeuchi K,Reue K(2009)Biochemistry,physiology,and genetics of GPAT,AGPAT,and lipin enzymes in triglyceride synthesis.Am J Physiol-Endoc M 296:E1195-E1209
Tamaki H,Shimada A,Ito Y,Ohya M,Takase J,Miyashita M,Miyagawa H,Nozaki H,Nakayama R,Kumagai H(2007)LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.Journal of Biological Chemistry282:34288-34298
Thines E,Weber RW,Talbot NJ(2000)MAP kinase and protein kinase A-dependent mobilization of triacylglycerol and glycogen during appressorium turgor generation by Magnaporthe grisea.The Plant cell 12:1703-1718
Veneault-Fourrey C,Lambou K,Lebrun MH(2006)Fungal Pls1 tetraspanins as key factors of penetration into host plants:a role in re-establishing polarized growth in the appressorium?Fems Microbiol Lett 256:179-184
Virag A,Lee MP,Si HY,Harris SD(2007)Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans.Molecular microbiology 66:1579-1596
Wang CS,Leger RJS(2007)The Metarhizium anisopliae perilipin homolog MPL1 regulates lipid metabolism,appressorial turgor pressure,and virulence.Journal of Biological Chemistry282:21110-21115
Xu YJ,Luo F,Gao Q,Shang Y,Wang C(2015)Metabolomics reveals insect metabolic responses associated with fungal infection.Analytical and bioanalytical chemistry
Zheng W,Zhao Z,Chen J,Liu W,Ke H,Zhou J,Lu G,Darvill AG,Albersheim P,Wu S,Wang Z(2009)A Cdc42 ortholog is required for penetration and virulence of Magnaporthe grisea.Fungal Genetics and Biology 46:450-460