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雷州半岛湖栖鳍虾虎鱼群体分化研究
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摘要
为探索雷州半岛两岸新纪录种湖栖鳍虾虎鱼遗传差异,选取COI基因和控制区序列作为分子标记分析两岸群体遗传结构。COI结果显示:变异位点29个;T、C、A、G碱基组成分别为:27.8%、31.0%、23.7%、17.5%,且A+T(51.5%)高于C+G(48.5%);单倍型多样性比较,九龙山群体(0.695)>高桥群体(0.324);遗传分化分析表明东、西群体分化显著(Snn=0.0.591,0.01高桥群体(0.661);遗传分化分析表明东、西群体分化极显著(Snn=0.970,P<0.001),Fst为0.315(P<0.001),Nm值为0.55;AMOVA分析揭示群体内变异程度高于群体间;NJ聚类分析均存在按照采样点聚类的现象。可见,湖栖鳍虾虎鱼的九龙山群体与高桥群体分化现象较明显。
In this study, we compared two Gobiopterus lacustris populations which from eastern and wesrern Leizhou Peninsula based on COI gene and D-loop region. The results were shown as follows : there were 29 variable sites in 619 bp COI sequences. The frequencies of T, C, A, and G were 27.8%, 31.0%, 23.7% and 17.5%, respectively.The haplotype diversity of Jiulongshan population(0.695) were higher than Gaoqiao population(0.324). Genetic differentiation analysis indicated that the eastern and western populations were differentiated significantly(Snn=0.591,0.01
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