高效液相色谱-荧光法测定牛羊组织中阿苯达唑及其代谢物的残留量
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- 英文论文题名：Determination of Albendazole and Its Main Metabolites Residues in Cattle and Ovine Tissues by HPLC-FLD
- 论文作者：吴宁鹏 ; 彭丽 ; 孟蕾 ; 李慧素
- 英文论文作者：Wu Ning-peng ; Peng Li ; Meng Lei ; Li Hui-su ; Henan Institute of Veterinary Drug and Feeds Control
- 年：2016
- 作者机构：河南省兽药饲料监察所;
- 论文关键词：高效液相色谱-荧光法 ; 牛羊组织 ; 阿苯达唑及其代谢物
- 英文论文关键词：HPLC-FLD ; cattle and ovine tissues ; albendazole and its main metabolites
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：O657;S859.84
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：7
- 文件大小：834k
- 原文格式：O
- 会议级别：全国
摘要
建立了同时测定牛羊组织中阿苯达唑及其代谢物的高效液相色谱-荧光检测方法。试样中待测物经乙酸乙酯提取,酸性氧化铝固相萃取柱和MCX固相萃取柱净化,高效液相色谱-荧光法测定,以外标法定量。药物在5~2000μg/L浓度范围内线性关系良好,相关系数均大于0.999。方法的检出限为0.02 mg门kg,定量限为0.05 mg/kg,在不同组织中添加浓度范围为0.05~10 mg/1g,其平均回收率为60.6~119.1%,相对标准偏差在1.7~19.1%之间。方法抗干扰能力强、灵敏度高,适用于牛羊组织中阿苯达唑及其代谢物残留量的测定。
A method based on high performance liquid chromatography with fluorescence detector(HPLC-FLD) has been developed for the determination of albendazole and its main metabolites residues in cattle and ovine tissues.The analytes were extracted with ethyl acetate,and cleaned by Alumina-A and MCX solid phase extraction cartridges.Quantification of the analytes were achieved by HPLC-FLD using external standard method.The calibration curves of the drugs were linear in the concentration ranges of 5~2000μg/L with correlation coefficients more than 0.999.Detection Limits and quantification limits of the method for the analytes were 0.02 mg/kg and 0.05 mg/kg in cattle and ovine tissues.The average recoveries ranged from 60.6~ 119.1%at the spiked level of 0.05~10 mg/kg in tissues.The relative standard deviation was in the rang of 1.7~19.1%.With the advantages of good anti-interference ability and sensitivity,the method adapts to the determination of albendazole and its main metabolites residues in cattle and ovine tissues.
A method based on high performance liquid chromatography with fluorescence detector(HPLC-FLD) has been developed for the determination of albendazole and its main metabolites residues in cattle and ovine tissues.The analytes were extracted with ethyl acetate,and cleaned by Alumina-A and MCX solid phase extraction cartridges.Quantification of the analytes were achieved by HPLC-FLD using external standard method.The calibration curves of the drugs were linear in the concentration ranges of 5~2000μg/L with correlation coefficients more than 0.999.Detection Limits and quantification limits of the method for the analytes were 0.02 mg/kg and 0.05 mg/kg in cattle and ovine tissues.The average recoveries ranged from 60.6~ 119.1%at the spiked level of 0.05~10 mg/kg in tissues.The relative standard deviation was in the rang of 1.7~19.1%.With the advantages of good anti-interference ability and sensitivity,the method adapts to the determination of albendazole and its main metabolites residues in cattle and ovine tissues.
引文
[1]周丽萍.动物组织中阿苯达陛代谢物多残留检测方法的研究[D].山东大学,2009.
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[4]Couneil Regulation No.2377/90 and Commission Regulation Nos.508/1999,2385/1999,2393/1999 and 807/2001[S].
[5]李俊锁,邱月明,王超.兽药残留分析[M].上海科学技术社,2002:459-95.
[6]伏旭,李培武,贺莉,等.酶联免疫吸附检测法的应用研究进展[J].氨基酸和生物资源,2012,34:41-44.
[7]Dowling G,Cantwell H,O'Keeffe M,et al.Multi-residue method for the determination of benzimidazoles in bovine liver[J].Anal Chim Acta,2005,529:285-292.
[8]Moreno L,Lopez-Urbina M T,Farias C,et al.A high oxfendazole dose to control porcine cysticercosis:Pharmacokinetics and tissue residue profiles[J].Food and Chemical Toxicology,2012,50:3189-3825.
[9]Lange H,Eggers,Bircher J.Increased Systemic Availability of Albendazole when Taken with a Fatty Meal[J].Eur J Clin Pharmacol,1988,34:315-317.
[10]张素霞,沈建忠,等.牛肝中苯并咪唑类药物残留的高效液相色谱检测方法[J].中国兽药杂志,2005,39:18-21.
[11]郭强,常孝勇.动物组织中苯并咪唑类药物检测的LC-MS/MS法研究[J].河南农业科学2012,41:152-156.
[12]张小军,郑斌,张虹,等.超高效液相色谱-串联质谱法测定草鱼肉中阿苯达唑及其代谢物残留[J].分析化学,2011,39,815-820.
[13]Balizs G.Determination of benzimidazole residues using liquid Chromatography and tandem mass speetrometry[Jl.J Chromatogr B,1999,727:167-177.
[14]Markus J,Sherma J.Gas-chromarographic-mass spectrometric confirmatory Method for albendazole residues in cattle liver[J].JAOACInt,1992,75:1135-113
[2]Moreno L,Imperiale F,Mottier L,et al.Comparison of milk residue profiles after oral and subcutaneousadministration of benzimidazole anthelmintics to dairy cows[J].Anal Chim Acta,2005,536:91-99
[3]Botsoglou N A,Fletouris D J.Drug residues and Public health.In Drug Residuesin Food:Pharmaeology,Food Safety,and Analysis:Botsoglou,N.A,Fletouris,D.J.,Eds.:Mareel Dekker[N].NewYork,2001:269-298
[4]Couneil Regulation No.2377/90 and Commission Regulation Nos.508/1999,2385/1999,2393/1999 and 807/2001[S].
[5]李俊锁,邱月明,王超.兽药残留分析[M].上海科学技术社,2002:459-95.
[6]伏旭,李培武,贺莉,等.酶联免疫吸附检测法的应用研究进展[J].氨基酸和生物资源,2012,34:41-44.
[7]Dowling G,Cantwell H,O'Keeffe M,et al.Multi-residue method for the determination of benzimidazoles in bovine liver[J].Anal Chim Acta,2005,529:285-292.
[8]Moreno L,Lopez-Urbina M T,Farias C,et al.A high oxfendazole dose to control porcine cysticercosis:Pharmacokinetics and tissue residue profiles[J].Food and Chemical Toxicology,2012,50:3189-3825.
[9]Lange H,Eggers,Bircher J.Increased Systemic Availability of Albendazole when Taken with a Fatty Meal[J].Eur J Clin Pharmacol,1988,34:315-317.
[10]张素霞,沈建忠,等.牛肝中苯并咪唑类药物残留的高效液相色谱检测方法[J].中国兽药杂志,2005,39:18-21.
[11]郭强,常孝勇.动物组织中苯并咪唑类药物检测的LC-MS/MS法研究[J].河南农业科学2012,41:152-156.
[12]张小军,郑斌,张虹,等.超高效液相色谱-串联质谱法测定草鱼肉中阿苯达唑及其代谢物残留[J].分析化学,2011,39,815-820.
[13]Balizs G.Determination of benzimidazole residues using liquid Chromatography and tandem mass speetrometry[Jl.J Chromatogr B,1999,727:167-177.
[14]Markus J,Sherma J.Gas-chromarographic-mass spectrometric confirmatory Method for albendazole residues in cattle liver[J].JAOACInt,1992,75:1135-113