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大米蛋白质组学研究进展
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摘要
稻谷是世界上最重要的粮食作物之一,约占世界膳食能量总供给量的20%。在众多植物中,大米作为单子叶植物的代表,其蛋白质组学的研究数量仅次于双子叶植物拟南芥,位列第二。随着蛋白质组学技术的发展和大米蛋白质组学数据库的建立,2000年以来,大米生长发育和胁迫应答等方向成为了大米蛋白质组学研究的热点。这些研究通过双向电泳(2-DE)、质谱(MS)等技术,较为完整的监控了在大米生长发育和胁迫应答中起主要功能的蛋白质以及蛋白质修饰等。而蛋白质组学在大米传统加工中却鲜有应用,如大米在传统加工前后差异蛋白质的鉴定、加工过程中蛋白质的修饰和相互作用等。本文综述了蛋白质组学主要技术的发展及其在大米生长发育、胁迫应答、基因修饰、储藏品质变化等方面的应用,旨在总结大米蛋白质组学的发展,并为今后蛋白质组学在大米传统加工的应用提供建议。
Rice is a critically important food crop plant on our planet,which provides 20%of the world's dietary energy supply.Plant proteomics studies have been confined to using model plant species such as Arabidopsis for dicots and rice for monocots.The number of rice proteomic studies is second only to Arabidopsis.With the development of proteomic technologies and the construction of the rice proteome database,rice proteomics studies to date have focused mainly on development of rice and stress response since 2000.The technique of proteome using two-dimensional electrophoresis(2-DE) and mass spectrum(MS) to monitor global changes that occur in the protein which play an important role in rice development,stress response and modification.But there is a limited amount of information on proteomic studies about conventional processes,especially the differential protein profile,modification and interaction.In this review,the current status of proteomic technologies and the application of proteomic in rice growth and development,stress response,genetically modified organisms,and storage quality are discussed.This review is intended to make a summing up of the advances in rice proteomic and offering advances for further application of rice proteomic in traditional processing.
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