高温胁迫及外源水杨酸对大丽花花瓣XTHs基因表达的影响
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- 英文论文题名：Effects of High Temperature and Exogenous Salicylic Acid on XTHs Genes Expression of Dahlia Petals
- 论文作者：管华 ; 张萍萍 ; 李坚 ; 孔周阳 ; 赵川乐 ; 谈建中
- 英文论文作者：GUAN Hua ; ZHANG Ping-ping ; LI Jian ; KONG Zhou-yang ; ZHAO Chuan-le ; TAN Jian-zhong ; Department of Horticulture ; College of Architecture ; Soochow University
- 年：2016
- 作者机构：苏州大学建筑学院园艺系;
- 论文关键词：大丽花 ; 高温胁迫 ; 水杨酸 ; 木葡聚糖内转糖苷酶/水解酶(XTH) ; 基因表达
- 英文论文关键词：Dahlia ; High temperature stress ; Salicylic acid ; Xyloglucan endotransglycosylase/hydrolase(XTH) ; Gene expression
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.261
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：500k
- 原文格式：O
- 会议级别：全国
摘要
木葡聚糖内转糖苷酶/水解酶(Xyloglucan endo-transglucosylase/hydrolase,XTH)是一种可以改变植物细胞壁结构的双功能酶,在花瓣衰老过程中起重要的调节作用。为探明高温胁迫及外源水杨酸(SA)与大丽花花瓣衰老之间关系的分子机理,本研究以大丽花‘单瓣黄'品种为供试材料,应用实时荧光定量PCR技术,分析了外源SA预处理对高温胁迫下大丽花花瓣DpXTH1和DpXTH2基因的表达变化。结果表明,在高温胁迫下,大丽花花瓣DpXTH1和DpXTH2的表达量在盛花期显著下降,仅为常温对照区的2.13%和1.78%;外源SA预处理后,DpXTH1和DpXTH2的表达量有所增加,分别为对照区的16.21%和36.22%。说明施用SA可以缓解高温胁迫对XTHs基因表达的抑制作用,有助于延缓高温胁迫下大丽花花瓣的衰老进程。
XTH(Xyloglucan endo-transglucosylase/hydrolase) is a bi-functional enzyme that can transform the structure of cell wall,and play an important regulating effect during petal aging process in plants.In order to ascertain the molecular mechanism of the relationship between high temperature stress,exogenous salicylic acid(SA) and petal senescence in dahlia pinnata Cav.,this study selected dahlia variety ' Danbanhuang' as experimental materials.Under high temperature stress and exogenous SA pre-treatment,the expression change of DpXTHl and DpXTHZ genes related to senescence of dahlia petals were analyzed by real-time fluorescent quantitative PCR technology.The results showed that the expression levels of DpXTH1 and DpXTHl in dahlia petals declined significantly under heat stress during bloom stage,only 2.13%and 1.78%of that in the control group under normal temperature respectively;while after exogenous SA pre-treatment,the expression levels of DpXTHl and DpXTHl increased,16.21%and 36.22%of that in the control group respectively.This indicated that SA could alleviate the inhibition of high temperature on XTHs expression,and delay the senescence process of dahlia petals under high temperature.
XTH(Xyloglucan endo-transglucosylase/hydrolase) is a bi-functional enzyme that can transform the structure of cell wall,and play an important regulating effect during petal aging process in plants.In order to ascertain the molecular mechanism of the relationship between high temperature stress,exogenous salicylic acid(SA) and petal senescence in dahlia pinnata Cav.,this study selected dahlia variety ' Danbanhuang' as experimental materials.Under high temperature stress and exogenous SA pre-treatment,the expression change of DpXTHl and DpXTHZ genes related to senescence of dahlia petals were analyzed by real-time fluorescent quantitative PCR technology.The results showed that the expression levels of DpXTH1 and DpXTHl in dahlia petals declined significantly under heat stress during bloom stage,only 2.13%and 1.78%of that in the control group under normal temperature respectively;while after exogenous SA pre-treatment,the expression levels of DpXTHl and DpXTHl increased,16.21%and 36.22%of that in the control group respectively.This indicated that SA could alleviate the inhibition of high temperature on XTHs expression,and delay the senescence process of dahlia petals under high temperature.
引文
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12.Yokoyama R,Uwagaki Y,Sasaki H,et al.Biological implications of the occurrence of 32 members of the XTH(xyloglucan endotransglucosylasc/hydrolase)family of proteins in the bryophyte Physcomitrella patens[J].The Plant Journal,2010,64:658-669.
13.Zhang Pingping,Kan Xuejin,Lijian,et al.Identification of senescence-associated protein DpXTHl and its gene cloning in dahlia petals[J].Agricultural Science and Technology,2015(7):1490-1493.
14.朱晓芳.拟南芥细胞壁半纤维素结合铝的机制及其调控[D].杭州:浙江大学,2014.
2.Choi J Y,Seo Y S,Su J K,et al.Constitutive expression of CaXTH3,a hot pepper xyloglucan endotransglucosylase/hydrolase,enhanced tolerance to salt and drought stresses without phenotypic defects in tomato plants(Solarium lycopersicum cv.Dotaerang)[J].Plant Cell Reports,2011,30(5):867-877.
3.Enzymic A M.Characterization of two recombinant xyloglucan endotransglucosylase/hydrolase(XTH)proteins of Arabidopsis and their effect on root growth and cell wall extension[J].Journal of Experimental Botany,2009,60(13):3959-3972.
4.Fry S C,Smith R C,Rrenwick K F,et al.Xyloglucan endotransglycosylase,a new wall-loosening enzyme activity from plants[J].Biochemical Journal,1992,282:821-828.
5.Haba P D L,Mata L D L,Molina E,et al.High temperature promotes early senescence in primary leaves of sunflower(Helianthus annuus L.)plants[J].Canadian Journal of Plant Science,2014,94:659-669.
6.Lee D,Polisensky D H,Braam J.Genome-wide identification of touch-and darkness-regulated Arabidopsis genes;a focus on calmodulin-like and XTH genes[J].New Phytologist,2005,165:429-444.
7.刘安成,王庆,庞长民.我国大丽花园艺学研究进展[J].北方园艺,2010,(11):225-228.
8.Mirza H,Kamrun N,Md Mahabub A,et al.Physiological,biochemical,and molecular mechanisms of heat stress tolerance in plants[J].International Journal of Molecular Sciences,2013,14(5):9643-9684.
9.Nishitani K.The role of endoxyloglucan transferase in the organization of plant cell walls[J].International Review of Cytology,1997,173:157-206.
10.孙悦,王蕾,姬筱雅,等.大丽花花瓣生长与衰老相关基因DpXTH的表达分析[J].江苏农业科学,2012,40(7):28-31.
11.王蕾.大丽花衰老相关基因XTH的表达特征和功能分析[D].苏州:苏州大学,2013.
12.Yokoyama R,Uwagaki Y,Sasaki H,et al.Biological implications of the occurrence of 32 members of the XTH(xyloglucan endotransglucosylasc/hydrolase)family of proteins in the bryophyte Physcomitrella patens[J].The Plant Journal,2010,64:658-669.
13.Zhang Pingping,Kan Xuejin,Lijian,et al.Identification of senescence-associated protein DpXTHl and its gene cloning in dahlia petals[J].Agricultural Science and Technology,2015(7):1490-1493.
14.朱晓芳.拟南芥细胞壁半纤维素结合铝的机制及其调控[D].杭州:浙江大学,2014.