新型QuEChERS方法结合液相色谱串联质谱快速测定猪肝中的β-受体激动剂残留
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- 英文论文题名：Rapid determination of β-agonists residuals in pig liver by LC-MS/MS combined with novel QuEChERS method
- 论文作者：曲斌 ; 耿士伟 ; 陆桂萍 ; 蒋天梅 ; 毕昊容 ; 姜加华 ; 陈蓉 ; 郭良雪
- 英文论文作者：QU Bin ; GENG Shi-wei ; LU Gui-ping ; JIANG Tian-mei ; BI Hao-rong ; JIANG Jia-hua ; CHEN Rong ; GUO Liang-xue ; Jiangsu Quality Inspection and Testing Center for Animal Products ; China Pharmaceutical University ; School of Sciences ; Agilent technologies(China) Co.Ltd
- 年：2016
- 作者机构：江苏省畜产品质量检验测试中心;中国药科大学理学院;安捷伦科技(中国)有限公司;
- 论文关键词：β-受体激动剂 ; 残留 ; QuEChERS ; 液质联用
- 英文论文关键词：β-agonists ; residuals ; QuEChERS ; LC-MS/MS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：8
- 文件大小：596k
- 原文格式：O
- 会议级别：全国
摘要
目的:建立了一种基于新型QuEChERS材料测定猪肝中-受体激动剂的方法。方法:猪肝样品经酶解、酸化乙腈提取后,提取液用增强基质去脂质材料(EMR-lipid)脱脂,盐析浓缩后经液相色谱质谱联用仪测定,稳定同位素内标法定量。结果:19种β-受体激动剂在0.5~10.0μg/kg范围内线性关系良好,高中低浓度日内、日间精密度小于20%,回收率在60~120%之间。结论:本方法简便快速,适宜于大批量样品的快速筛选和定量分析。
Objective:To develop a method to determine the |3-agonists residuals in pig liver based on a novel QuEChERS material.Methods:The sample of pig liver was enzymatic hydrolysis and extracted by acidified acetonitrile.The extraction was cleaned-up by enhanced matrix removal(EMR-lipid) material.After polish and salting out,the analytes were detected by LC-MS/MS.Stable isotopes were used as internal standard.Results:19 P-agonists were detected at the range between 0.5 μg/kg and10.0 μg/kg with good linearity.The intra- and inter-precision were lower than 20%,and the recoveries were between 60%and 120%with three high,medium and low concentrations.Conclusions:The method is simple,rapid and effective.It is suitable to rapid screen and quantitative large scale samples.
Objective:To develop a method to determine the |3-agonists residuals in pig liver based on a novel QuEChERS material.Methods:The sample of pig liver was enzymatic hydrolysis and extracted by acidified acetonitrile.The extraction was cleaned-up by enhanced matrix removal(EMR-lipid) material.After polish and salting out,the analytes were detected by LC-MS/MS.Stable isotopes were used as internal standard.Results:19 P-agonists were detected at the range between 0.5 μg/kg and10.0 μg/kg with good linearity.The intra- and inter-precision were lower than 20%,and the recoveries were between 60%and 120%with three high,medium and low concentrations.Conclusions:The method is simple,rapid and effective.It is suitable to rapid screen and quantitative large scale samples.
引文
[1]Shelley R,Salpeter MD,Nicholas S,et al.Meta-analysis:effect of long-actingβ-agonists on severe asthma exacerbations and asthma-related deaths.Annals of Internal Medicine,2006,144(12):904-912
[2]Andersom DB,Veenhuizen EL,Wagner JF,et al.The effect of ractopamine hydrochloride on nitrogen retention,growth performance and carcass composition of beef cattle.J Anim Sci,1989,67:222-225
[3]Koeberl DD,Li ST,Dai J,et al.β2-agonists enhance the efficacy of simultaneous enzyme replacement therapy in murine pompe disease.Mol Genet Metab,2012,105:221-227
[4]中华人民共和国农业部第235号公告《动物性食品中兽药最高残留限量》,2002
[5]潘云山,聂建荣,朱铭立,等。高效液相色谱串联三重四极杆质谱检测饲料中10种β-受体激动剂,广东农业科学,2010,11:252-254
[6]李阳,苏晓鸥,王瑞国,等。超高效液相色谱-串联质谱法同时测定绵羊唾液中14种β-受体激动剂,分析化学,2013,4l(6):899-904
[7]聂建荣,朱铭立,连槿,等。高效液相色谱-串联质谱法检测动物尿液中的15种β-受体激动剂,色谱,2010,28(8):759-764
[8]李莹莹,宋永青,郭文萍,等。超高效液相色谱-串联质谱方法同时测定猪肉中7种β-受体激动剂残留量,肉类研究,2012.26(5):25-29
[9]刘佳,谢云峰,任丹丹,等。反相液相色谱-串联质谱法检测猪肝中26种β-受体激动剂类药物残留,分析化学,2014,42(10):1486-1492
[10]徐丽君,卢艳芬,唐巍,等。超高效液相色谱-串联质谱法同时测定牛肉和牛肝中5种β-受体激动剂,分析测试技术与仪器,2012,18(3):140-148
[11]张晓荣,刘拉平,杨蓉。UPLC-ESI-SPE质谱法测定牛乳中9种-β受体激动剂残留量,中国乳品工业,2013,41(11):41-44
[12]刘思洁,崔勇,石矛,等。固相萃取-同位素稀释超高效液相色谱-串联质谱法测定动物源性食品中24种β2受体激动剂,食品安全质量检测学报,2015,6(5):1726-1734
[13]应永飞,朱聪英,陈慧华,等。多壁碳纳米管分散固相萃取结合MS/MS-LC测定饲料中11种β-受体激动剂,饲料加工与检测,2013,49(9):68-73
[14]周悦榕,李丹妮,顾欣。酶联免疫吸附分析法测定猪、鸡粪便中11种β2-受体激动剂残留。中国兽药杂志,2015,49(8):39-45
[15]朱坚,李波,方晓明,等。气相色谱-质谱法测定肝、肾和肉中11种β-受体激动剂残留量,质谱学报,2005,26(3):129-137
[16]孙雷,张骊,朱永林,等。超高效液相色谱-串联质谱法检测动物源性食品中残留的9种β-受体激动剂,色谱,2008,26(6):709-713
[17]刘畅,陈燕,李晓雯,等。高效液相色谱-四级杆-飞行时间质谱法同时测定猪肉中31种β-受体激动剂,中国食品卫生杂志,2014,26(5):464-469
[18]Anastassiades M,Lehotay S J,Stajnbaher D,et al.Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce.J AOAC Int,2003,86(2):412-431
[19]曲斌。QuEChERS在动物源性食品兽药残留检测中的研究进展。食品科学,2013,34(5):327-331
[20]曲斌,陈蓉,耿士伟,等。液质联用技术在兽药多类别、多组分残留分析中的应用研究进展。食品安全质量检测学报,2015,6(5):1654-1660
[21]Ulrey WD,Burnett TJ,Brunelle SL,et al.Determination and confirmation of parent and total ractopamine in bovine,swine,and turkey tissues by liquid chromatography with tandem mass spectrometry:final action 2011.23.J AOAC Int,2013,96(4):917-924
[2]Andersom DB,Veenhuizen EL,Wagner JF,et al.The effect of ractopamine hydrochloride on nitrogen retention,growth performance and carcass composition of beef cattle.J Anim Sci,1989,67:222-225
[3]Koeberl DD,Li ST,Dai J,et al.β2-agonists enhance the efficacy of simultaneous enzyme replacement therapy in murine pompe disease.Mol Genet Metab,2012,105:221-227
[4]中华人民共和国农业部第235号公告《动物性食品中兽药最高残留限量》,2002
[5]潘云山,聂建荣,朱铭立,等。高效液相色谱串联三重四极杆质谱检测饲料中10种β-受体激动剂,广东农业科学,2010,11:252-254
[6]李阳,苏晓鸥,王瑞国,等。超高效液相色谱-串联质谱法同时测定绵羊唾液中14种β-受体激动剂,分析化学,2013,4l(6):899-904
[7]聂建荣,朱铭立,连槿,等。高效液相色谱-串联质谱法检测动物尿液中的15种β-受体激动剂,色谱,2010,28(8):759-764
[8]李莹莹,宋永青,郭文萍,等。超高效液相色谱-串联质谱方法同时测定猪肉中7种β-受体激动剂残留量,肉类研究,2012.26(5):25-29
[9]刘佳,谢云峰,任丹丹,等。反相液相色谱-串联质谱法检测猪肝中26种β-受体激动剂类药物残留,分析化学,2014,42(10):1486-1492
[10]徐丽君,卢艳芬,唐巍,等。超高效液相色谱-串联质谱法同时测定牛肉和牛肝中5种β-受体激动剂,分析测试技术与仪器,2012,18(3):140-148
[11]张晓荣,刘拉平,杨蓉。UPLC-ESI-SPE质谱法测定牛乳中9种-β受体激动剂残留量,中国乳品工业,2013,41(11):41-44
[12]刘思洁,崔勇,石矛,等。固相萃取-同位素稀释超高效液相色谱-串联质谱法测定动物源性食品中24种β2受体激动剂,食品安全质量检测学报,2015,6(5):1726-1734
[13]应永飞,朱聪英,陈慧华,等。多壁碳纳米管分散固相萃取结合MS/MS-LC测定饲料中11种β-受体激动剂,饲料加工与检测,2013,49(9):68-73
[14]周悦榕,李丹妮,顾欣。酶联免疫吸附分析法测定猪、鸡粪便中11种β2-受体激动剂残留。中国兽药杂志,2015,49(8):39-45
[15]朱坚,李波,方晓明,等。气相色谱-质谱法测定肝、肾和肉中11种β-受体激动剂残留量,质谱学报,2005,26(3):129-137
[16]孙雷,张骊,朱永林,等。超高效液相色谱-串联质谱法检测动物源性食品中残留的9种β-受体激动剂,色谱,2008,26(6):709-713
[17]刘畅,陈燕,李晓雯,等。高效液相色谱-四级杆-飞行时间质谱法同时测定猪肉中31种β-受体激动剂,中国食品卫生杂志,2014,26(5):464-469
[18]Anastassiades M,Lehotay S J,Stajnbaher D,et al.Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce.J AOAC Int,2003,86(2):412-431
[19]曲斌。QuEChERS在动物源性食品兽药残留检测中的研究进展。食品科学,2013,34(5):327-331
[20]曲斌,陈蓉,耿士伟,等。液质联用技术在兽药多类别、多组分残留分析中的应用研究进展。食品安全质量检测学报,2015,6(5):1654-1660
[21]Ulrey WD,Burnett TJ,Brunelle SL,et al.Determination and confirmation of parent and total ractopamine in bovine,swine,and turkey tissues by liquid chromatography with tandem mass spectrometry:final action 2011.23.J AOAC Int,2013,96(4):917-924