高产甘露醇柠檬明串珠菌的筛选及培养基优化的研究

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• 英文论文题名：Study on thescreening of Leuconostoc citreum highly yielding mannitol and optimization of the culture medium
• 论文作者：郑琳 ; 程雅韵 ; 金清
• 英文论文作者：Zheng Lin ; Cheng Yayun ; Jin Qing ; College of Agriculture ; Yanbian University
• 年：2016
• 作者机构：延边大学农学院;
• 论文关键词：甘露醇 ; 柠檬明串珠菌 ; 甲基磺酸乙酯 ; 碳源 ; 氮源
• 英文论文关键词：mannitol ; leuconostoc citreum ; ethylmethyl sulfonate ; carbon source ; nitrogen source
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：TQ923
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：112k
• 原文格式：O
• 会议级别：全国

摘要

目的:以实验室保藏菌种柠檬明串珠菌95为实验菌株,通过化学诱变快速、高通量筛选高产甘露醇的诱变菌株,并研究碳、氮源对诱变菌株甘露醇生产能力的影响。方法:利用甲基磺酸乙酯(EMS)作为化学诱变剂诱变柠檬明串珠菌,确定EMS诱变的最佳条件。经初筛与复筛后采用薄层层析检测筛选菌株的甘露醇生产能力。对诱变菌株进行培养基成分优化,改变发酵培养基中果糖浓度、氮源种类和碳氮比等条件,利用高效液相色谱法进行甘露醇含量的测定。结果:EMS诱变处理的最佳条件为EMS浓度0.5%,处理时间30min。经EMS处理后共筛选出了3株诱变菌株,分别为95-8、95-9和95-12,其中95-9的甘露醇生产能力最高。对该菌株发酵培养基中的碳、氮源进行优化,最优条件为果糖浓度150g/L,蛋白胨作为氮源,碳氮比5:1。该条件下甘露醇的产量可达72.18g/L。讨论:本实验仅对诱变菌株进行了筛选,可对其进行生理、形态特征以及发酵特性等方面的深入研究。同时还可对菌株的其它发酵条件做进一步的优化,以期为甘露醇的工业化生产提供理论依据。
Objective:The present study used Leuconostoc citreum 95,a strain preserved in the laboratory,to screen out mutant strainshighly yielding mannitol by rapid and high-throughput chemical mutation,and investigate the effect of carbon and nitrogen sources on the mannitol-producing capacity of mutant strains.Method:Ethylmethane sulfonate(EMS) was used as a chemical mutagento induce mutation of Leuconostoc citreumand the optimal condition of EMS-inducedmutation was identified.After primary and secondary screening,mannitol-pruducing capacity of screened strains was determined using thin layer chromatography(TLC).The medium of mutant strains was optimized by changing conditions such as fructose concentration,nitrogen source typeand carbon-nitrogen ratio,and the mannitol content was measured using high performance liquid chromatography.Results:The optimal condition of EMS treatment was EMS concentration of 0.5%with a treatment period of 30 min.AfterEMS treatment,totally three mutant strains were screened out,including Leuconostoc citreum 95-8,Leuconostoc citreum95-9,and Leuconostoc citreum 95-12.Among them,Leuconostoc citreum 95-9 showed the highest mannitol-producing capacity.Optimization of the carbon and nitrogen sources in the medium of this strain showed that the optimal condition was a fructose concentration of 150g/Lwith peptone taken as nitrogen source and carbon-nitrogen ratio of 5:1.Under this condition,the output of mannitol reached72.18g/L.Discussion;In the present study,we only screened mutant strains and carried out indepth exploration of their physiological,morphological characteristics and fermentation characteristics.Meanwhile,we further optimized other fermentation conditions of these strains to provide a theoretical basis for the industrial production of mannitol.

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