福建省闽东地区37株沙门菌PFGE指纹图谱分析及药敏研究
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- 论文作者：郭良 ; 郑锦利 ; 韩善梅 ; 肖秀飞
- 年：2016
- 作者机构：福建医科大学附属闽东医院检验科;
- 论文关键词：沙门菌 ; 脉冲场凝胶电泳(PFGE) ; 分子分型 ; 耐药分析
- 英文论文关键词：Salmonella ; pulsed field gel electrophoresis(PFGE) ; molecular typing ; drug resistance analysis
- 会议召开时间：2016-11-25
- 会议录名称：湖南中医药大学学报2016/专集：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会论文集
- 语种：中文
- 分类号：R446.5
- 学会代码：HNZY
- 会议名称：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会
- 会议地点：中国广东珠海
- 主办单位：国际数字医学会、Digital Chinese Medicine
- 学会名称：湖南中医药大学期刊杂志社
- 页数：2
- 文件大小：2206k
- 原文格式：D
- 会议级别：全国
摘要
目的通过分析福建省闽东地区分离的沙门菌脉冲场凝胶电泳(PFGE)指纹图谱,研究其分型特征及耐药情况。方法收集2014-2015年间37株临床分离沙门菌株,其中鼠伤寒沙门菌28株,肠炎沙门菌9株。先采用PFGE技术生成电泳酶切指纹,再用Bio Numerics软件对图谱进行聚类分析。同时对分离菌株进行药敏试验,结果用WHONET5.1软件进行分析。结果 28株鼠伤寒沙门菌PF GE指纹图谱按照100%的相似度可分为16个PFGE型,其中P1型有11株,P4、P5型各有2株,P1型为优势型别;9株肠炎沙门菌PFGE指纹图谱按照100%的相似度可分为3个PFGE型,其中P1型6株,P2型有1株,P3型有2株,P1型为优势型别。37株沙门菌对头孢类的头孢曲松、头孢他啶、头孢哌酮/舒巴坦、头孢吡肟,喹诺酮类的左旋氧氟沙星具有较高的敏感性,对青霉素类的氨苄西林,氨苄西林/舒巴坦敏感性较低。结论沙门菌PFGE分型技术具有分辨率高,重复性好等优点,可广泛用于沙门菌感染疫情的溯源和医院感染的防控。
Objective To analyze the Salmonella isolation area of Fujian Province Fujian pulsed field gel electrophoresis(PFGE) fingerprint, study the features and types of drug resistance. Methods 2014-2015 from 37 strains of Salmonella isolates, including 28 strains of Salmonella typhimurium were mainly isolated from infants with diarrhea, 9 strains of Salmonella enteritidiswere mainly isolated from adult infectious diarrhea. The Salmonella chromosome DNA was digested by Xba I enzyme, using H9812 as a molecular weight standard pulse field gel electrophoresisfingerprint analysis of enzyme cutting. Using PFGE technology, clustering analysis was conducted with Bio Numerics software. At the same time, drug sensitive test outcomings isolates were analyzedby WHONET5.1. Results 28 strains Salmonella typhimurium PF GE map according to the 100% similarity can be divided into 16 PFGE types, including 11 strains of type P1, P4, P5 type 2 strains, P1 was better. Potential type; 9 strains of Salmonella enteritidis PFGE patterns according to the similarity of 100% can be divided into 3 types, including 6 strains of type P1, P2 has a strain that P3 has 2 strains, P1 is the dominant type of 37 strains of Salmonella to cephalosporins ceftriaxone, ceftazidime, Cefoperazone / sulbactam, cefepime, quinolones levofloxacin has higher sensitivity, topenicillin, ampicillin, ampicillin / sulbactam sensitivity lower. Conclusions PFGE divided approach with high resolution, the advantages of good repeatability can be widely applied to diseaseorigin and early prevention of Salmonella infection.
Objective To analyze the Salmonella isolation area of Fujian Province Fujian pulsed field gel electrophoresis(PFGE) fingerprint, study the features and types of drug resistance. Methods 2014-2015 from 37 strains of Salmonella isolates, including 28 strains of Salmonella typhimurium were mainly isolated from infants with diarrhea, 9 strains of Salmonella enteritidiswere mainly isolated from adult infectious diarrhea. The Salmonella chromosome DNA was digested by Xba I enzyme, using H9812 as a molecular weight standard pulse field gel electrophoresisfingerprint analysis of enzyme cutting. Using PFGE technology, clustering analysis was conducted with Bio Numerics software. At the same time, drug sensitive test outcomings isolates were analyzedby WHONET5.1. Results 28 strains Salmonella typhimurium PF GE map according to the 100% similarity can be divided into 16 PFGE types, including 11 strains of type P1, P4, P5 type 2 strains, P1 was better. Potential type; 9 strains of Salmonella enteritidis PFGE patterns according to the similarity of 100% can be divided into 3 types, including 6 strains of type P1, P2 has a strain that P3 has 2 strains, P1 is the dominant type of 37 strains of Salmonella to cephalosporins ceftriaxone, ceftazidime, Cefoperazone / sulbactam, cefepime, quinolones levofloxacin has higher sensitivity, topenicillin, ampicillin, ampicillin / sulbactam sensitivity lower. Conclusions PFGE divided approach with high resolution, the advantages of good repeatability can be widely applied to diseaseorigin and early prevention of Salmonella infection.
引文
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[2]Centers for Disease Cont rol and Prevention out break of Salmonella infections associated wit h peanut butterand peanut butter2containing p roduct s22United States,200822009[J].MMWR,2009,58(4):85290.
[3]Ribot EM,Fair MA,Gautom R,et al.Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7,Salmonella,and Shigella for Pulse Net[J].Foodborne Pathog Dis.2006,Spring;3(1):59-67.
[4]谢一俊,郑国,陈建辉,等.福建197522003年沙门菌监测结果分析[J].海峡预防医学杂志,2004,(6):7210.
[5]Swaminat han B,Barrett TJ,Hunter SB,et al.Pulse Net:the mo2lecular subt yping net work for foodborne bacterial disease surveil2lance,U nited States[J].Emerg Infect Dis,2001,7(3):3822389.
[6]Liebana E,Clouting C,Garcia-Migura L,et al.Multiple genetic typing of Salmonella Enteritidis phage-types 4,6,7,8 and 13 aisolates from animals and humans in the UK[J].Vet Microbiol,2004,100(3-4):189-195.