中国东南沿海城市水产品中抗生素抗性基因的污染研究
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- 英文论文题名：Occurrence of antibiotic resistance genes in aquatic products in the southeast coastal cities,China
- 论文作者：巴永兵 ; 牛丽 ; 张昭寰 ; 潘迎捷 ; 赵勇
- 英文论文作者：Ba Yongbing ; Niu Li ; Zhang Zhaohuan ; Pan Yingjie ; Zhao Yong ; College of Food Science and Technology ; Shanghai Ocean University ; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation ; Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai) ; Ministry of Agriculture
- 年：2016
- 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室(上海);
- 论文关键词：抗生素抗性基因 ; 东南沿海地区 ; 食品安全 ; 水产品
- 英文论文关键词：antibiotic resistance genes ; southeast coastal cities ; food safety ; aquatic products
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：R155
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：110k
- 原文格式：O
- 会议级别：全国
摘要
[目的]由于对人体健康构成的潜在微信,食品中抗生素抗性基因(ARGs)的污染日益受到人们的关注,本文探究了水产品中ARGs的污染水平。[方法]选取上海、杭州、宁波、厦门、福州五个东南沿海城市17种共93个水产品作为研究对象,利用PCR方法检测37种耐药基因的存在性,包括5种β-内酰胺类,8种四环素类,8种氨基糖苷类,7种喹诺酮类,5种氯霉素类,4种磺胺类。[结果]tetS,srtA,strB,aadA,sul1,and sul2这6种ARGs被选定为中国东南沿海水产品中的主要污染ARGs,这6类ARGs在五个城市均有检出,并且检出率均大于40%。上海检出的ARGs种类最多,为16种,宁波最少,只有10种ARGs被检出。此外,淡水水产品的ARGs平均检出数量要高于海产品。[结论]水产品已成为ARGs的重要储存库,淡水产品包含的ARGs数量要明显高于海产品,需引起人们足够的重视。
[Objective]Antibiotic resistance genes(ARGs) has drawn increasing attention due to its great threat to human health.In this study,the occurrence of ARGs in aquatic products were investigated.[Methods]A total of 17 species of 93 aquatic product samples were collected from five southeast coastal cities of China including Shanghai,Hangzhou,Ningbo,Fuzhou,Xiamen.Conventional PCR was used to determine the presence of all ARGs including five β-lactam ARGs,eight tetracycline ARGs,eight aminoglycoside ARGs,seven quinolone ARGs,five chloramphenicol ARGs and four sulfonamide ARGs.[Results]tetS,strA,strB,aadA,sul1,and sul2 were considered as the dominnat ARGs in aquatic products in the southeast coastal area of China,they were observed in all five cities samples and were most frequently detected in aquatic products,with detection frequencies of these genes were all more than 40%.Shanghai contained most of the test ARGs(16 ARGs),whereas Ningbo contained least of the target ARGs,only 10 ARGs were detected.The average number of detected ARGs were generally higher in freshwater aquatic products than in seafoods.[Conclusion]this study comprehensively describe ARGs pollution in aquatic products of southeast coastal area and note that aquatic product has become an potential repository to spread ARGs,These results showed a trend thatfreshwater aquatic productswere more severely pollutedby ARGs than seafoods,hence needing more attention.
[Objective]Antibiotic resistance genes(ARGs) has drawn increasing attention due to its great threat to human health.In this study,the occurrence of ARGs in aquatic products were investigated.[Methods]A total of 17 species of 93 aquatic product samples were collected from five southeast coastal cities of China including Shanghai,Hangzhou,Ningbo,Fuzhou,Xiamen.Conventional PCR was used to determine the presence of all ARGs including five β-lactam ARGs,eight tetracycline ARGs,eight aminoglycoside ARGs,seven quinolone ARGs,five chloramphenicol ARGs and four sulfonamide ARGs.[Results]tetS,strA,strB,aadA,sul1,and sul2 were considered as the dominnat ARGs in aquatic products in the southeast coastal area of China,they were observed in all five cities samples and were most frequently detected in aquatic products,with detection frequencies of these genes were all more than 40%.Shanghai contained most of the test ARGs(16 ARGs),whereas Ningbo contained least of the target ARGs,only 10 ARGs were detected.The average number of detected ARGs were generally higher in freshwater aquatic products than in seafoods.[Conclusion]this study comprehensively describe ARGs pollution in aquatic products of southeast coastal area and note that aquatic product has become an potential repository to spread ARGs,These results showed a trend thatfreshwater aquatic productswere more severely pollutedby ARGs than seafoods,hence needing more attention.
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