干旱胁迫下一氧化氮和过氧化氢诱导万寿菊不定根形成中蛋白激酶活性的变化
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- 英文论文题名：Changes of CDPK and MAPK Activities during Nitric Oxide and Hydrogen Peroxide Induced-Adventitious Root Development in Marigold under Drought Stress
- 论文作者：牛丽涓 ; 李凤娇 ; 廖伟彪
- 英文论文作者：NIU Li-juan ; LI Feng-jiao ; LIAO Wei-biao ; College of Horticulture ; Gansu Agricultural University
- 年：2016
- 作者机构：甘肃农业大学园艺学院;
- 论文关键词：万寿菊 ; 干旱胁迫 ; 一氧化氮(NO) ; 过氧化氢(H_2O_2) ; 不定根形成
- 英文论文关键词：Marigold ; Hydrogen peroxide(H_2O_2) ; Nitric oxide(NO) ; Adventitious rooting
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S681.9
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：809k
- 原文格式：O
- 会议级别：全国
摘要
一氧化氮(NO)和过氧化氢(H_2O_2)已被大量研究发现为重要的信号分子,广泛参与到动植物体内各种信号转导过程。本试验以万寿菊为材料研究了一氧化氮和过氧化氢诱导其外植体不定根形成中钙依赖蛋白激酶(CDPK)和促分裂素原活化蛋白激酶(MAPK)活性的变化。本试验在聚乙二醇(PEG)模拟干旱胁迫条件下,以蒸馏水处理为对照,分别用最适浓度PEG、PEG+硝普钠(SNP,NO供体)、PEG+H_2O_2及PEG+SNP+H_2O_2对万寿菊外植体进行处理。结果表明,相比于对照,干旱处理下的外植体内CDPK和MAPK活性显著降低,PEG+SNP或PEG+H_2O_2处理的活性显著提高。PEG+SNP+H_2O_2处理的CDPK和MAPK活性显著高于PEG+SNP或PEG+H_2O_2单独处理,表明不定根的发生过程中SNP和H_2O_2具有协同诱导效应。可见,干旱胁迫下NO和H_2O_2在诱导万寿菊不定根形成过程中CDPK和MAPK活性显著提高。
Several lines of experiments proved that nitric oxide(NO) and hydrogen peroxide(H_2O_2) are crucial signal molecules involved signal physiological processes.Marigold was used to understand the changes of CDPK and MAPK with NO and H_2 O_2 to improve the development of adventitious roots in drought conditions.The results showed that maximal biological responses of H_2O_2 and NO donor sodium nitropmsside(SNP) were 2000μM and 10μM;Moreover,the effect of NO and H_2O_2 were dependent on adventitious roots organogenesis of explants.Additionally,the enzyme activity of explants treated with NO and H_2O_2 simultaneously were significantly higher those of explants treated with NO or H_2O_2 alone.Besides,NO treatments enhanced endogenous H_2O_2 levels in hypocotyls.Together,the results indicate that NO and H_2O_2 relieved the drought stress and developed the adventitious roots involved the adventitious roots development of marigold explants under the drought case.
Several lines of experiments proved that nitric oxide(NO) and hydrogen peroxide(H_2O_2) are crucial signal molecules involved signal physiological processes.Marigold was used to understand the changes of CDPK and MAPK with NO and H_2 O_2 to improve the development of adventitious roots in drought conditions.The results showed that maximal biological responses of H_2O_2 and NO donor sodium nitropmsside(SNP) were 2000μM and 10μM;Moreover,the effect of NO and H_2O_2 were dependent on adventitious roots organogenesis of explants.Additionally,the enzyme activity of explants treated with NO and H_2O_2 simultaneously were significantly higher those of explants treated with NO or H_2O_2 alone.Besides,NO treatments enhanced endogenous H_2O_2 levels in hypocotyls.Together,the results indicate that NO and H_2O_2 relieved the drought stress and developed the adventitious roots involved the adventitious roots development of marigold explants under the drought case.
引文
1.廖伟彪,肖洪浪,张美玲.2009.一氧化氮和过氧化氢对地被菊扦插生根的影响[J].园艺学报,36(11):1643-1650.
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4.万丙良,查中萍,戚华雄.2009.钙依赖的蛋白激酶与植物抗逆性[J].生物技术通报,1:7-10.
5.陈硕,陈珈.2001.植物中钙依赖蛋白激酶(CDPKS)的结构与功能[J].植物学通报,18(2):143-148.
6.姜义宝,崔国文,李红.2005.干旱胁迫下外源钙对苜蓿抗旱相关生理指标的影响[J].草业学报,5(1):32-36.
7.薄惠,王棚涛,董发才,等.2005.拟南芥保卫细胞中茉莉酸甲酯诱导的H_2O_2产生与MAPK信号转导体系的可能关系[J].植物生理学通讯,41(4):439-443.
8.郭晓君.蛋白质电泳实验技术[M].北京:科学出版社,1999.
9.Jonak C,Kiegerl S,Ligterink W,Barker P J,Huskisson N S,Hirt,H.1996.Stress signaling in plants:a mitogen-activated protein kinase pathway is activated by cold and drought[J].Proceedings of the National Academy of Sciences,93(20):11274-11279.
10.Frank L Guide to protein purification.Methods in enzymology,Academic press,1990.
11.Liao W,Xiao H,Zhang M.2009.Role and relationship of nitric oxide and hydrogen peroxide in adventitious root development of marigold[J].Acta physiol plant,31(6):1279-1289.
2.吕东,张骁,江静,等.2005.N0可能作为H_2O_2的下游信号介导ABA诱导的蚕豆气孔关闭[J].植物生理与分子生物学学报,31(1):62-70.
3.吴宇芬,周卫川,陈宏,等.1996.H_2O_2对蕹菜种子活力影响的研究[J].福建省农科院学报,11(2):25-30.
4.万丙良,查中萍,戚华雄.2009.钙依赖的蛋白激酶与植物抗逆性[J].生物技术通报,1:7-10.
5.陈硕,陈珈.2001.植物中钙依赖蛋白激酶(CDPKS)的结构与功能[J].植物学通报,18(2):143-148.
6.姜义宝,崔国文,李红.2005.干旱胁迫下外源钙对苜蓿抗旱相关生理指标的影响[J].草业学报,5(1):32-36.
7.薄惠,王棚涛,董发才,等.2005.拟南芥保卫细胞中茉莉酸甲酯诱导的H_2O_2产生与MAPK信号转导体系的可能关系[J].植物生理学通讯,41(4):439-443.
8.郭晓君.蛋白质电泳实验技术[M].北京:科学出版社,1999.
9.Jonak C,Kiegerl S,Ligterink W,Barker P J,Huskisson N S,Hirt,H.1996.Stress signaling in plants:a mitogen-activated protein kinase pathway is activated by cold and drought[J].Proceedings of the National Academy of Sciences,93(20):11274-11279.
10.Frank L Guide to protein purification.Methods in enzymology,Academic press,1990.
11.Liao W,Xiao H,Zhang M.2009.Role and relationship of nitric oxide and hydrogen peroxide in adventitious root development of marigold[J].Acta physiol plant,31(6):1279-1289.