百合花中β-葡萄糖和木糖糖苷酶基因的克隆及序列分析
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- 英文论文题名：Cloning and Bioinformatics Analysis of LoGlu and LoXyl Gene from Lilium ‘Siberia’
- 论文作者：张昕 ; 余让才 ; 范燕萍
- 英文论文作者：ZHANG Xin ; YU Rang-cai ; FAN Yan-ping ; Flower Research Center ; South China Agricultural University ; College of Life Sciences ; South China Agricultural University ; College of Forestry and Landscape Architecture ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学花卉研究中心;华南农业大学生命科学学院;华南农业大学林学与风景园林学院;
- 论文关键词：百合 ; β-葡萄糖糖苷酶 ; 木糖糖苷酶 ; 基因克隆 ; 序列分析
- 英文论文关键词：Lilium ‘Siberia’ ; LoGlu ; LoXyl ; Gene cloning ; Sequence Analysis
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.29
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：7
- 文件大小：2061k
- 原文格式：O
- 会议级别：全国
摘要
以‘西伯利亚'百合(Lilium‘Siberia')为材料,克隆得到β-葡萄糖糖苷酶LoGlu和木糖糖苷酶LoXyl基因。LoGlu的ORF长1461bp,编码486个氨基酸(aa)。LoXyl的ORF长2316bp,编码771个氨基酸。蛋白家族结构域分析LoGlu和LoXyl分别含有糖苷水解酶家族1和糖苷水解酶家族3的序列。亚细胞定位信号分析结果显示LoGlu和LoXyl均不存在核定位信号,LoGlu可能定位于细胞质,LoXyl可能定位于线粒体。氨基酸同源序列比对结果表明,LoGlu和LoXyl的同源性较低,可能与保守性基序差异有关。二级结构预测分析结果显示LoGlu含有36.83%的α螺旋残基,18.52%的延伸链,39.83%的无规则卷曲;LoXyl含有21.66%的α螺旋残基,28.02%的延伸链,50.32%的无规则卷曲。
Two glycosidase gene,named LoGlu 和 LoXyl was isolated from the flowers of Lilium' Siberia',The LoGlu gene has an ORF of 1461 bp coding 486 amino acids.The LoXyl gene has an ORF of 2316 bp coding 771 amino acids.Protein predicted domain indicated that LoGlu and LoXyl have CH1 and CH3 sites.It predicted that LoGlu in the cytoplasm and LoXyl in the mitochondrion by Subcellular localization.The result of amino acid sequence alignment reveals that the protein LoGlu 和LoXyl poss little homology,because of the difference of Conserved motifs.The secondary structure prediction results manifests that LoGlu has 36.83%α-helix,18.52%extended strand,39.83%random coil;LoXyl has 21.66%α-helix,28.02%extended strand,50.32%random coil.
Two glycosidase gene,named LoGlu 和 LoXyl was isolated from the flowers of Lilium' Siberia',The LoGlu gene has an ORF of 1461 bp coding 486 amino acids.The LoXyl gene has an ORF of 2316 bp coding 771 amino acids.Protein predicted domain indicated that LoGlu and LoXyl have CH1 and CH3 sites.It predicted that LoGlu in the cytoplasm and LoXyl in the mitochondrion by Subcellular localization.The result of amino acid sequence alignment reveals that the protein LoGlu 和LoXyl poss little homology,because of the difference of Conserved motifs.The secondary structure prediction results manifests that LoGlu has 36.83%α-helix,18.52%extended strand,39.83%random coil;LoXyl has 21.66%α-helix,28.02%extended strand,50.32%random coil.
引文
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13.Jacobson Ft,Schlein Y,Eisenberger C.2001.The biological function of sandfly and Leishmania glycosidases[J].Medical Microbiology and Immunology,190:51-55.
14.Alfonso F,Emndez M.1991.Synthesis and modification of carbohydrates using glycosidases and lipases[J].Topics in Current Chemistry,186:1-20.
15.Carl SR,Stephen GW.2000.Glycosidase mechanisms[J].Current Opinion in Chemical Biology,4:573-580.
2.Muhlemann J K,Klempien A,Dudareva N.2014.Floral volatiles:from biosynthesis to function[J].Plant Cell and Environment,37(8):1936-1949.
3.Raguso RA.2008.Wake up and smell the roses:the ecology and evolution of floral scent[J].Annual Review of Ecology,Evolution,and Systematics 39:549-569.
4.Unsicker SB,Kuneit G,Gershenzon J.2009.Protective perfumes:the role of vegetative volatiles in plant defense against herbivores[J].Current Opinion In Plant Biology 12:479-485.
5.Ali JG,Alborn HT,Campos-Herrera R,Kaplan F,Duncan LW,Rodriguez-Saona C,Koppenhofer AM,Stelinski LL2012.Subterranean,herbivore-induced plant volatile increases biological control activity of multiple beneficial nematode species in distinct habitats[J].PLoS ONE 7:e38146.
6.Hiltpold I,Turlings TCJ.2012.Manipulation of chemically mediated interactions in agricultural soils to enhance the control of crop pests and to improve crop yield[J].Journal of Chemical Ecology 38:641-650.
7.Huang M,Sanchez-Moreiras AM,Abel C,Sohrabi R,Lee S,Gershenzon J,Tholl D.2012.The major volatile organic compound emitted from Arabidopsis thaliana flowers,the sesquiterpene(E)-b-caryophyllene,is a defense against a bacterial pathogen[J].New Phytologist 193:997-1008.
8.Baldwin IT,Halitschke R,Paschold A,von Dahl CC,Preston CA.2006.Volatile signaling in plant-plant interactions:"Talking trees"in the genomics era[J].Science 311:812-815.
9.Dudareva N,Negre F,Nagegowda DA,Orlova I.2006.Plant volatiles:recent advances and future perspectives[J].Critical Reviews in Plant Sciences 25:417-440.
10.Vickers CE,Gershenzon J,Lerdau MT,Loreto F.2009.A unified mechanism of action for volatile isoprenoids in plant abiotic stress[J].Nature Chemical Biology 5:283-291.
11.Winterhalter P,Skouroumounis GK(1997)Glycoconjugated aroma compounds:occurrence,role and biotechnological transformation.In:Scheper T(ed)Advances in biochemical engineering/biotechnology.Springer,Berlin Heidelberg New York,pp74-105.
12.Sarry J E,Giinata Z.Plant and microbial glycoside hydrolases Volatile release from glycosidic aroma precursors[J].Food Chemistry,2004,87:509-521.
13.Jacobson Ft,Schlein Y,Eisenberger C.2001.The biological function of sandfly and Leishmania glycosidases[J].Medical Microbiology and Immunology,190:51-55.
14.Alfonso F,Emndez M.1991.Synthesis and modification of carbohydrates using glycosidases and lipases[J].Topics in Current Chemistry,186:1-20.
15.Carl SR,Stephen GW.2000.Glycosidase mechanisms[J].Current Opinion in Chemical Biology,4:573-580.