一种胶原基生物质复合材料

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• 英文论文题名：A Biomass Composite Based on Collagen
• 论文作者：孟雪萍 ; 张柠 ; 张腾飞 ; 何茜婵 ; 范夏莲 ; 汤克勇
• 英文论文作者：MENG Xueping ; ZHANG Ning ; ZHANG Tengfei ; HE Xichan ; FAN Xialian ; TANG Keyong ; College of Materials Science and Engineering ; Zhengzhou University
• 年：2016
• 作者机构：郑州大学材料科学与工程学院;
• 论文关键词：胶原 ; 丝素 ; 羟基磷灰石 ; 复合材料 ; 制备
• 英文论文关键词：collagen ; silk fibroin ; hydroxyapatite ; composite ; preparation
• 会议召开时间：2016-07-27
• 会议录名称：2016第十一届全国皮革化学品学术交流会暨中国皮革协会技术委员会第21届年会摘要集
• 英文会议录名称：Abstracts of 2016 the 11th National Conference on Leather Chemicals & 21st Annual Convention of China Leather Science and Technology Commission
• 语种：中文
• 分类号：TB332
• 学会代码：ZGHG
• 会议名称：2016第十一届全国皮革化学品学术交流会暨中国皮革协会技术委员会第21届年会
• 会议地点：中国山东济南
• 主办单位：中国皮革协会技术委员会、中国皮革协会皮革化工专业委员会、中国化工学会精细化工专业委员会
• 学会名称：中国化工学会
• 页数：2
• 文件大小：78k
• 原文格式：O
• 会议级别：全国

摘要

以I型胶原(Col)和丝素蛋白(SF)为原料,采用原位沉积法使钙、磷在其表面沉积形成羟基磷灰石(HA),制得了胶原/丝素/羟基磷灰石(Col/SF/HA)复合材料。以戊二醛(GA)和生物交联剂1-乙基-3-(3-二甲胺基丙基)碳二亚胺碘甲烷盐/N-羟基琥珀酰亚胺(EDC/NHS)进行交联,制备了Col/SF/HA-GA和Col/SF/HA-EDC/NHS两种复合材料。FT-IR和XRD测试表明,以丝素蛋白和胶原的羧基为沉积位点,HA沉积于胶原及丝素表面。钙磷比结果说明,复合物中无机相与天然生物骨磷灰石相似。随着反应温度的升高及交联剂的引入,HA在胶原和丝素表面沿c轴择优生长,结晶度逐渐增大。扫描电镜(SEM)显示复合材料具有不规则孔洞结构,经EDC/NHS交联后,孔洞增多且HA沉积更均匀致密,在50℃和60℃时,其多孔状结构发生塌陷。可通过调控反应温度及交联剂制备不同构成及结构的Col/SF/HA复合材料。
Collagen/silk fibroin/hydroxyapatite(Col/SF/HA) composites were prepared by in situ precipitation with collagen and silk fibroin at different temperatures.Chemical cross-linker glutaraldehyde(GA) or biological cross-linker ethyl-3-(3-dimethylaminopropyl) carbodiimide iodide salt/N-hydroxysuccinimide(EDC/NHS) were used to obtain Col/SF/HA-GA composite and Col/SF/HA-EDC/NHS composite.With Col and Col/SF as templates,the effects of temperature and cross-linkers on the resultant composites were investigated.Fourier transformed infrared spectroscopy(FTIR) and X-ray diffraction analysis(XRD) indicated that carboxyl group may act as the deposition cite,making calcium and phosphorus ions deposit on the surface of collagen and silk fibroin.Combining the calcium/phosphate ratio test,the inorganic phase of composite was similar to apatite in natural bone.With the increase of temperature and the addition of cross-linkers,the orientation of hydroxyapatite(HA) along the c-axis was better as well as the higher crystallinity.Scanning electron microscope(SEM) showed irregular micropores in the composites.After EDC/NHS cross-linking,more pores appeared and the deposition of HA was more compact.At 50 ℃ and 60 ℃,however,the porous structure collapsed.The work is concerned with the factors affecting the resultant composites,such as templates(collagen,silk fibroin/collagens),temperature,and cross-linkers,in order to regulate the composition and microstructure of the Col/SF/HA composites.

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