调控喉鳞状细胞癌Livin基因在裸鼠移植瘤表达的实验研究

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• 英文论文题名：The Expression regulated of Livin on the transplantation tumor of laryngeal squamous cell carcinoma cell in nude mice
• 论文作者：冯俊 ; 李丽 ; 杨久梅 ; 彭涛
• 英文论文作者：Feng Jun ; Li Li ; Yang Jiumei ; Peng Tao ; From the Department of ENT ; Second Clinical Medical College of North Sichuan Medical College ; Nanchong Central Hospital ; Department of Pathology ; North Sichuan Medical College
• 年：2016
• 作者机构：川北医学院第二临床医学院耳鼻咽喉科,南充市中心医院耳鼻咽喉科;川北医学院病理教研室;
• 论文关键词：Livin基因 ; 喉鳞状细胞癌 ; 裸鼠
• 英文论文关键词：Livin ; Squamous cell carcinoma ; nude mice
• 会议召开时间：2016-12-01
• 会议录名称：中国医师进修论文选编
• 语种：中文
• 分类号：R739.65
• 学会代码：BCLK
• 会议名称：2016年12月中国医师进修学术交流会
• 会议地点：中国北京
• 主办单位：中国医师进修编委会
• 学会名称：百川利康（北京）国际医学研究院
• 页数：4
• 文件大小：719k
• 原文格式：D
• 会议级别：全国

摘要

目的:利用反义Livin多核苷酸调低喉鳞状细胞癌裸鼠移植瘤Livin基因的表达以探讨其对喉鳞状细胞癌凋亡的影响。方法:(1)雌性裸鼠(BALB/c-nu/nu),分成Livin AS-DNs(Livin反义多核苷酸)、Mis-DNs、Sen-DNs、对照组,每组7只,于裸鼠背部皮下注射1x10~(4-5)个hep-2细胞成瘤;(2)采用Western blotting检查AS-DNs、Mis-DNs组、Sen-DNs组、对照组瘤组织Livin蛋白;(3)利用TUNEL染色法对各组瘤体中细胞凋亡情况进行分析。结果:(1)Livin蛋白检查量最低为AS组(15.02±1.68)%,Sen组、Mis组、Control组明显高于AS组,AS组与其他组比,P<0.05,有统计学意义;(2)裸鼠瘤体内喉鳞癌细胞的凋亡指数最高为(20.15±3.1)%,为AS-DNs组,Sen组、Mis组、Control组明显低于AS组,AS组与其他组比,P<0.05,差异有统计学意义;(3)采用AS-DNs治疗组的肿瘤生长较Sen组、Mis组、Control组慢,AS组与其他组比,P<0.05,差异有统计学意义;结论:反义Livin多核苷酸在裸鼠移植瘤内促进了喉鳞状细胞癌的凋亡,抑制了肿瘤的生长。
Objective: To investigate the effect of apoptosis of the antisense polynucleotide( AS-DNs) targeting Livin to transplantation tumor of laryngeal squamous cell carcinoma cell line( Hep-2) in nude mice. Methods:(1) Female BALB/c-nu/nu mice were divided into four groups( n= 7 in each group):(a) Control group,(b) Livin AS-DNs group,(c) Sen-DNs group, and(d) Mis-DNs group. About 1 ×10~(4-5) Hep-2 cells were subcutaneously inoculated in dorsal space of the mice.(2)The expression of Livin protein in tumor was examined by Western blotting.(3) TUNEL assay was used to analyze cell apoptosis in tumor of nude mice. Results:(1) The Livin protein expression of AS-DNs was(15.02 ± 1.68)% to the untreated group,which was much lower than other group. There are statistical significance in the group AS-DNs with Control group、Sen-DNs group, and Mis-DNs group(P<0.05).(2) The apoptotic cells was notably higher in AS-DNs group than that of other group. The AI(Apoptotic Index) was(20.15 ± 3.1)% in AS-DNs group,which was significantly higher than that of the other groups.(3)Tumor growth rate was slower in AS-DNs group than that of Control group、Sen.-DNs group, and Mis-DNs group. Conclusion: AS-DNs of Livin could promote apoptosis of laryngeal squamous cell carcinoma and inhibit tumor growth in vivo.

引文

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