海洋来源真菌ZJ-M-5中可“天然降解”的多聚内酯类化合物

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• 英文论文题名：Naturally degradable macrocyclic polylactone from the marine-derived fungus,Ascotricha sp.ZJ-M-5
• 论文作者：李占林 ; 张金玲 ; 王文静 ; 李丹毅 ; 华会明
• 英文论文作者：LI Zhan-lin ; ZHANG Jin-ling ; WANG Wen-jing ; LI Dan-yi ; HUA Hui-ming ; Key Laboratory of Structure-Based Drug Design and Discovery ; School of Traditional Chinese Materia Medica ; Shenyang Pharmaceutical University
• 年：2016
• 作者机构：沈阳药科大学"基于靶点的药物设计与研究"教育部重点实验室,中药学院;
• 论文关键词：刺囊壳属 ; 聚酮 ; 大环多聚内酯 ; 天然降解
• 英文论文关键词：Ascotricha ; polyketide ; macrocyclic polylactone ; natural degradation
• 会议召开时间：2016-09-27
• 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
• 语种：中文
• 分类号：R915
• 学会代码：YYWS
• 会议名称：中国药学会第十三届青年药学科研成果交流会
• 会议地点：中国江西南昌
• 主办单位：中国药学会
• 学会名称：中国药学会
• 页数：6
• 文件大小：631k
• 原文格式：O
• 会议级别：全国

摘要

从海洋来源真菌Ascotricha sp.ZJ-M-5在去除Mg~(2+)离子的查氏培养基静置培养物中分离得到3个新的链状聚酮来源的多聚酯类化合物ascotrichesters A-C(1-3)、1个新的大环多聚内酯ascotrichalactone A(4),一个新的聚己酮orthosporinin(5)及其二聚物diorthosporinin(6),以及2个已知的生合成前体(-)-orthosporin(7)和(-)-6-hydroxymellein(8)。通过对化合物4在不同pH值水溶液中稳定性以及ZJ-M-5次生代谢产物随时间变化情况的考察,我们认为大环多聚内酯结构4应该是真菌真正产生的代谢产物,而具有与其相同聚酮片段连接顺序的链状结构(3),及化合物5应该是其"天然降解产物",这与之前学者提出的生合成路径是相反的。此外,我们还测试了上述化合物的体外抗肿瘤活性及化合物3-4的抑菌活性。
Four new polyketide-derived polyesters,including three linear ones ascotrichesters A-C(1-3) and one macrocyclic ascotrichalactone A(4),and the known biosynthetic building blocks(-)-orthosporin(7) and(-)-6-hydroxymellein(8),were isolated from the still culture product of the fiingus Ascotricha sp.ZJ-M-5 in Czapek Dox broth without Mg~(2+),along with a new hexaketide orthosporinin(5) and its visible light-catalyzed[2+2]dimerized derivative diorthosporinin(6).HPLC monitoring of the stability of 4 in H_2O disclosed the fact that macrocyclic lactone4,is the natural precursor which undergoes "natural degradation" to yield the linear polyester 3 and parallel reactions to afford 5.This result demonstrated that the previously proposed biosynthetic pathway for polyesters from linear to cyclic should be reversed.Additionally,the growth inhibitory effects of 1-8 against two human leukemia cancer cell lines and the antibacterial activities of compounds 3 and 4 were assessed.

引文

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