烟田常用杀虫剂对烟蚜茧蜂的毒力及其保护酶的影响
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- 英文论文题名：Effects of Conventional Insecticides on Toxicity and Protective Enzyme of Aphidius Gifuensis Ashmead in Tobacco Fields
- 论文作者：朱先志 ; 刘勇 ; 田雷 ; 刘莉 ; 宗浩 ; 高强 ; 徐蕊 ; 李兰芬 ; 张伟娜
- 英文论文作者：ZHU Xianzhi ; LIU Yong ; TIAN Lei ; LIU Li ; ZONG Hao ; GAO Qiang ; XU Rui ; LI Lanfen ; ZHANG Weina ; Yishui County Tobacco Company of Shandong Province ; Linyi Tobacco Company of Shandong Province
- 年：2016
- 作者机构：山东临沂烟草有限公司沂水分公司;山东临沂烟草有限公司;
- 论文关键词：杀虫剂 ; 烟蚜茧蜂 ; 毒力 ; 酶活性
- 英文论文关键词：insecticides ; Aphidius gifuensis Ashmead ; toxicity ; enzyme activity
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：6
- 文件大小：859k
- 原文格式：D
摘要
为了筛选对烟蚜茧蜂安全性高的药剂并明确烟田常用杀虫剂对烟蚜茧蜂保护酶的影响,本文测定了3种烟田常用杀虫剂对烟蚜茧蜂成虫的毒力以及不同浓度的吡虫啉对烟蚜茧蜂成虫体内超氧化物歧化酶、过氧化物酶和过氧化氢酶活性的影响。结果表明,杀虫剂对烟蚜茧蜂成虫的毒力大小顺序为:啶虫脒>高效氯氟氰菊酯>吡虫啉;从不同浓度的吡虫啉对烟蚜茧蜂生命活动的影响和烟蚜茧蜂的防御机制来看,除LC10浓度吡虫啉促进烟蚜茧蜂成虫体内POD活性提高和3个浓度处理后12 h成虫CAT活性显著提高外,其余处理浓度和时间对3种酶的活性呈现抑制作用。此结果为探明烟蚜茧蜂对杀虫剂的防御机制提供了一定的理论依据。
In order to screen the highly safety insecticides to Aphidius gifuensis Ashmead and know about the effects of conventional insecticides on protective enzyme of Aphidius gifuensis Ashmead in tobacco fields. Toxicity of three conventional insecticides to Aphidius gifuensis Ashmead was determined by residual film method. Effects of different concentrations of imidacloprid on SOD,POD and CAT activity of Aphidius gifuensis Ashmead was also determined by different methods. The result showed: the order from high toxicity to low toxicity was: acetamiprid >lambda-cyhalothrin >imidacloprid. In terms of the effects of different concentrations of imidacloprid on the life activities of Aphidius gifuensis Ashmead and their defense mechanism, the remaining treatments showed inhibition on enzyme activity except the LC10 concentrations of imidacloprid improved POD activity in adults and CAT activity increased significantly in adults after 12 h treatments of three concentration. This result provides theory basis to the defense mechanism of Aphidius gifuensis Ashmead against insecticides.
In order to screen the highly safety insecticides to Aphidius gifuensis Ashmead and know about the effects of conventional insecticides on protective enzyme of Aphidius gifuensis Ashmead in tobacco fields. Toxicity of three conventional insecticides to Aphidius gifuensis Ashmead was determined by residual film method. Effects of different concentrations of imidacloprid on SOD,POD and CAT activity of Aphidius gifuensis Ashmead was also determined by different methods. The result showed: the order from high toxicity to low toxicity was: acetamiprid >lambda-cyhalothrin >imidacloprid. In terms of the effects of different concentrations of imidacloprid on the life activities of Aphidius gifuensis Ashmead and their defense mechanism, the remaining treatments showed inhibition on enzyme activity except the LC10 concentrations of imidacloprid improved POD activity in adults and CAT activity increased significantly in adults after 12 h treatments of three concentration. This result provides theory basis to the defense mechanism of Aphidius gifuensis Ashmead against insecticides.
引文
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[16]陈立,徐汉虹.新型烟碱型杀虫剂吡虫啉作用机制研究进展[J].湖北农学院学报,1988,18(1):85-88.
[17]刘慧平,韩巨才,徐琴,等.杀虫剂对苹果黄蚜与七星瓢虫的毒力及选择性研究[J].中国生态农业学报,2007,15(2):126-129.
[18]Suh C P,Qrr D B,Duyn J W,et al.Effect of insecticides on Trichogramma exiguum(Trichogrammatidae:Hymenoptera)preimaginal development and adult survival[J].Journal of Economic Entomology,2000,93(3):577-583.
[19]蒋志胜,尚稚珍,万树青.光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响[J].昆虫学报,2003,46(1):22-26.
[20]查黎春.三种农药对天幕毛虫、舞毒蛾超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响[D].黑龙江:东北林业大学,2011.
[21]赵俊红,刘军侠,姜文虎,等.SO2胁迫对异色瓢虫的生长发育及保护酶的影响[J].河北农业大学学报,2011,34(1):87-91.
[22]杨琼,王淑会,张文慧,等.常用杀虫剂对异色瓢虫的毒力及其保护酶的影响[J].植物保护学报,2015,42(2):258-263.
[2]柴正群,况荣平,朱建青.烟蚜重寄生蜂种群动态初步研究[J].中国烟草科学,2013,34(1):61-65.
[3]Allen R G,Farmer K J,Sohal R S.Effect of diamide administration on longevity,oxygen consumption,superoxide dismutase,catalase,inorganic peroxides andglutathione in adult house fly[M].Musca domestica.Comp.Biochem.Physiol.C,1984,78:31-33.
[4]Allen R G,Balin A K.Oxidative influence ondevelopment and differentiation:an overview of a freeradical theory of development[M].Free Radic.Biol.Med.,1989,6(6):631-639.
[5]Bolter C J,Chefurka W.Extramitochondrial release of hydrogen peroxide from insect and mouse liver mitochondriausing the respiratory inhibitors phosphine,myxothiazol,andantimycin and spectral analysis of inhibited cytochromes[M].Arch.Biochem.Biophys,1990,278(1):65-72.
[6]陈常理,韦茂兔,李明江,等.温度对B型烟粉虱存活率和保护酶系的影响[J].浙江农业科学,2010(4):853-857.
[7]冯宏祖,刘映红,何林,等.阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响[J].植物保护学报,2008,35(6):530-536.
[8]王楠,张志春,王满囷,等.腐胺对小菜蛾幼虫生长及保护酶活力的影响[J].昆虫知识,2008,45(4):573-576.
[9]朱建兰,王国利,刘谨,等.四脊裸胞壳Dh菌株侵染对钩麦蛾幼虫体内保护酶的影响[J].草地学报,2008,16(2):121-125.
[10]刘修,张悦,王涛,等.阿维菌素类和烟碱类杀虫剂对烟草幼苗叶绿素含量的影响[J].中国烟草科学,2013,34(2):64-68.
[11]陈丹,陈德鑫,许家来,等.五种杀虫剂对烟草潜叶蛾的毒力测定[J].中国烟草科学,2013,34(2):37-40.
[12]农业标准出版研究中心.NY/T 1154.8—2007滤纸药膜法[S].北京:中国农业出版社,2007.
[13]沈文飚,徐朗莱,叶茂炳,等.氮蓝四哇光化还原法测定超氧化物歧化酶活性的适宜条件[J].南京农业大学学报,1996,19(2):101-102.
[14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]Giannopolitis C N,Ries S K.Superoxide dismutases.I.Occurrence in higher plants.Plant[J].Physiology,1977(59):309-314.
[16]陈立,徐汉虹.新型烟碱型杀虫剂吡虫啉作用机制研究进展[J].湖北农学院学报,1988,18(1):85-88.
[17]刘慧平,韩巨才,徐琴,等.杀虫剂对苹果黄蚜与七星瓢虫的毒力及选择性研究[J].中国生态农业学报,2007,15(2):126-129.
[18]Suh C P,Qrr D B,Duyn J W,et al.Effect of insecticides on Trichogramma exiguum(Trichogrammatidae:Hymenoptera)preimaginal development and adult survival[J].Journal of Economic Entomology,2000,93(3):577-583.
[19]蒋志胜,尚稚珍,万树青.光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响[J].昆虫学报,2003,46(1):22-26.
[20]查黎春.三种农药对天幕毛虫、舞毒蛾超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响[D].黑龙江:东北林业大学,2011.
[21]赵俊红,刘军侠,姜文虎,等.SO2胁迫对异色瓢虫的生长发育及保护酶的影响[J].河北农业大学学报,2011,34(1):87-91.
[22]杨琼,王淑会,张文慧,等.常用杀虫剂对异色瓢虫的毒力及其保护酶的影响[J].植物保护学报,2015,42(2):258-263.