广藿香浸膏的分子蒸馏分离-全二维气相色谱/飞行时间质谱法分析

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• 英文论文题名：Determination of volatile organic compounds in Patchouli Extract by molecular distillation and Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry
• 论文作者：邹西梅 ; 金晶 ; 万强 ; 刘剑 ; 杨靖
• 英文论文作者：ZOU Xi-mei ; JIN Jing ; WAN Qiagn ; LIU Jian ; YANG jing ; Technology Center ; China tobacco Guizhou Industrial Company Ltd. ; School of Food and Biological Engineering ; Zhengzhou University of Light Industry
• 年：2016
• 作者机构：贵州中烟工业有限责任公司技术中心;郑州轻工业学院食品与生物工程学院;
• 论文关键词：全二维气相色谱/飞行时间质谱 ; 广藿香 ; 超临界流体萃取 ; 分子蒸馏
• 英文论文关键词：comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GCTOFMS) ; patchouli ; supercritical co2 fluid extraction ; molecular distillation
• 会议召开时间：2016-12-01
• 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
• 语种：中文
• 分类号：TS264.3
• 学会代码：ZGYG
• 学会名称：中国烟草学会
• 页数：8
• 文件大小：1124k
• 原文格式：D

摘要

应用全二维气相色谱/飞行时间质谱(GC×GC-TOFMS)分析广藿香浸膏的挥发性成分,采用超临界CO2流体萃取获得广藿香浸膏,浸膏经分子蒸馏仪40℃蒸馏分离,所得的轻馏分经GC×GC-TOFMS分析,通过TOFMS谱库(自建谱库和NIST谱库)检索对照,结合包含结构信息的二维谱图以及人工质谱图解析、丰度比比对等定性手段,采用色谱峰面积归一化法计算各化学成分的相对含量。结果:分析得到匹配度大于750的组分有323个,含量占总量的89.45%,其中酮类67个,醇类52个,烯类40个,酸类37个,烷类36个,酯类34个,醛类17个,其它类40个。相对含量大于0.1%的组分69个,其中相对含量大于1%的组分11个,以广藿香酮含量最高,为21.75%,其次是广藿香醇、叶绿醇、异冰片醇、α-松油醇、γ-榄香烯、α-愈创木烯等。
A method was developed to analyze the volatile organic compounds(VOCs) in patchouli extract by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry(GC×GC-TOFMS). Patchouli sample was pretreated with Supercritical CO_2 fluid extraction and molecular distillation at 40 ℃, light distillates were detected by GC×GC-TOFMS. Columns with different polarity and selectivity were selected, Rxi-5sil MS is selected as fist dimension and combined with column of increasing polarity in the second dimension such as Rtx-200. Auto date processing by TOFMS software combined with mass spectrum analysis and ion ratio was used to identify the compounds. The result showed that 323 constituents were identified with similarity more than 750 in the spectrum, accounting for about 89.16% percent of the whole peak area, including 67 ketones,52 alcohols, 40 alkenes, 37 acids, 36 alkanes, 34 ethers, 17 aldehydes and 40 others. There were 11 compounds with area% above 1%, Pogostone(21.75%) and patchouli alcohol(18.94%) were the main constituents in patchouli extract.

引文

[1]Donelian A,Carlson L H C,Lopes T J,et al.Comparison of extraction of patchouli(pogostemon cablin)essential oil with supercritical CO2 and by steam distillation[J].The Journal of Supercritical Fluids,2009,2009(48):15-20.
    [2]胡海燕,彭劲甫,黄世亮,等.分子蒸馏技术用于广藿香油纯化工艺的研究[J].中国中药杂志,2004,29(4):320-322.
    [3]连宗衍,杨丰庆,李绍平.顶空固相微萃取-气相色谱质谱法定性定量分析广藿香中的挥发性成分[J].分析化学,2009,37(2):283-287.
    [4]林彦君,许莉陈,佳江川,等.藿香与广藿香挥发油化学成分GC-MS对比分析[J].中国实验方剂学杂志,2013,19(20):100-102.
    [5]Parastar H,Radowic J R,Jalali-Heravi M,et al.Resolution and Quantification of Complex Mixtures of Aromatic Hydrocarbons in Heavy Fuel Oil Sample by Means of GC×GC-TOFMS Combined to Multivariate Curve Resolution[J].Analytical chemistry,2011,2011(83):9289-9297.
    [6]王楠,张艺,李响,等.全二维气相色谱/飞行时间质谱分析不同产地的川芎挥发油[J].色谱,2010,28(4):329-335.
    [7]徐世娟,冒德寿,李智宇,等.全二维气相色谱-飞行时间质谱分析赖百当油的挥发性成分[J].光谱实验室,2012,29(4):2194-2199.
    [8]郭琨,周建,刘泽龙.全二维气相色谱-飞行时间质谱联用技术分析重馏分油中芳烃组成[J].色谱,2012,30(2):128-134.
    [9]Hoggard J C,Synovec R E.Automated Resolution of Nontarget Analyte Signals in GC×GC-TOFMS Data Using Parallel Factor Analysis[J].Analytical chemistry,2008,2008(80):6677-6688.
    [10]于娜娜,张丽坤,朱江兰,等.超临界流体萃取原理及应用[J].化工中间体,2011,2011(8):38-43.
    [11]高旭,陈鹏,洪林军,等.分子蒸馏技术在烟用香料提取分离中的应用[J].化学工程师,2009,2009(10):54-56.
    [12]何晓蕾,俞勇梅,李咸伟,等.全二维气相色谱/飞行时间质谱法快速定性分析飞灰样品中的二噁英[J].分析化学,2010,38(7):935-940.