矢车菊素-3-0-葡萄糖苷膜修饰脂质体抗硒性白内障作用研究
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- 英文论文题名：The anti-cataract effect of membrane-modified cyanidin-3-glycoside-loaded liposomes
- 论文作者：李翔 ; 季苗苗 ; 杨明 ; 张婧 ; 廖正根 ; 俞庆倩 ; 徐凤男
- 英文论文作者：LI Xiang ; Ji Miaomiao ; YANG Ming ; ZHANG Jing ; LIAO Zhenggen ; YU Qingqian ; XU Fengnan ; State Key laboratory of innovative drug and efficient energy-saving pharmaceutical equipment ; Jiangxi University of Traditional Chinese Medicine ; Key Laboratory of Modern Preparation of TCM ; Ministry of Education ; Jiangxi University of Traditional Chinese Medicine
- 年：2016
- 作者机构：江西中医药大学创新药物与高效节能降耗制药设备国家重点实验室;江西中医药大学现代中药制剂教育部重点实验室;
- 论文关键词：三甲基壳聚糖 ; 矢车菊素3-葡萄糖苷 ; 膜修饰脂质体 ; 抗白内障 ; 晶状体
- 英文论文关键词：N-trimethyl chitosan ; cyanidin-3-glycoside ; membrane-modified liposomes ; anti-cataract ; lens
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：4
- 文件大小：472k
- 原文格式：O
- 会议级别：全国
摘要
目的:制备矢车菊素-3-O-葡萄糖苷(C3G)膜修饰脂质体,并对其抗硒性白内障作用进行考察。方法:以自制三甲基壳聚糖(TMC)为脂质体膜修饰材料,采用逆相蒸发法制备TMC修饰的C3G脂质体(C3G-TCL);采用超滤法、粒径电位测定仪考察C3G-TCL理化性质;建立硒性白内障大鼠动物模型,采用裂隙灯检测大鼠晶状体浑浊度并测定生化指标,对C3G-TCL的抗白内障作用进行研究。结果:C3G-TCL粒径为(158.3±2.8)nm,Zeta电位为(31.7±1.0)mV,包封率为(53.7±0.2)%,抗白内障药效显示,与模型组相比C3G-TCL给药组的晶状体混浊明显延迟,与白内障相关生化指标得到显著改善。结论:C3G-TCL能在一定程度上通过对抗由亚硒酸钠导致的晶状体混浊的主要因素,起到预防及延缓白内障产生及发展的作用。
OBJECTIVE:To preparation membrane-modified cyanidin-3-O-glycoside(C3G)-loaded liposomes and investigate its anti-cataract effect.METHODS:Self-made N-trimethyl chitosan(TMC)was used as liposomal modifying material.TMC-modified C3G-loaded liposomes(C3G-TCL)were prepared by reverse-phase evaporation.The ultrafilter method,dynamic light scattering and electrophoretic light scattering were employed to study the physicochemical parameters of C3G-TCL.When the selenite-induced animal cataract was established,the opacity examination of lenses and cataract-related biochemical markers were determined.RESULTS:The particle size was(158.3±2.8)nm,zeta potential was(31.7±1.0) mV and the drug encapsulation efficiency achieved(53.7±0.2)%.The pharmacodynamic data showed that the onset of cataract in the C3G-TCL-treated group was significantly later than in the model group with significant improvement of the biomarkers.CONCLUSION:It was indicated the positive protective action of C3G-TCL to prevent or delay the cataract formation and development to a certain degree.
OBJECTIVE:To preparation membrane-modified cyanidin-3-O-glycoside(C3G)-loaded liposomes and investigate its anti-cataract effect.METHODS:Self-made N-trimethyl chitosan(TMC)was used as liposomal modifying material.TMC-modified C3G-loaded liposomes(C3G-TCL)were prepared by reverse-phase evaporation.The ultrafilter method,dynamic light scattering and electrophoretic light scattering were employed to study the physicochemical parameters of C3G-TCL.When the selenite-induced animal cataract was established,the opacity examination of lenses and cataract-related biochemical markers were determined.RESULTS:The particle size was(158.3±2.8)nm,zeta potential was(31.7±1.0) mV and the drug encapsulation efficiency achieved(53.7±0.2)%.The pharmacodynamic data showed that the onset of cataract in the C3G-TCL-treated group was significantly later than in the model group with significant improvement of the biomarkers.CONCLUSION:It was indicated the positive protective action of C3G-TCL to prevent or delay the cataract formation and development to a certain degree.
引文
[1]RESNIKOFF S,PASCOLINID,ETYA'ALE D,et al.Global data on visual impairment in the year 2002[J].Bull World Health Organ,2004,82:844-851.
[2]STINTXING FC,STINTZING AS,CARLE R,et al.Color and antioxidant properties of C3Ganidin-based anthoC3Ganin pigments[J].J Agric Food Chem,2002,50(21):6172-6181.
[3]MORIMISU Y,KUBOTA K,TASHIRO T,et al.Inhibitory effect of anthocyanins and colored rice on diabetic cataract formation in the rat lenses[J].Inter Congress Series,2002,1245:503-508.
[4]LUO QL,ZHAO JM,ZHANG XR,et al.Nanostructured lipid carrier(NLC)coated with chitosan oligosaccharides and its potential use in ocular drug delivery system[J].Int J Pharm,2011,403:185-191.
[5]SHRESTHA N,SHAHBAZI MA,ARAUJO F,et al.Chitosan-modified porous silicon microparticles for enhanced permeability of insulin across intestinal cell monolayers[J].Biomaterials,2014,35(25):7172-7179.
[6]WANG SL,ZHANG J,JIANG TY,et al.Protective effect of Coenzyme Qioagainst oxidative damage in human lens epithelial cells by novel ocular drug carriers[J].Int J Pharm,2011,403(1-2):219-229.
[7]LI X,LUO Y,LIAO ZG,et al.Effect of chitosan and its derivatives as coating materials on corneal permeability of the curcumin-loaded liposomes[J].Chin J Pharm(中国医药工业杂志),2015,46(3):265-269.
[8]ZHANG J,LUO YX,LIAO ZG,et al.Preparation of membrane-modified liposomes loaded with cyanidin-3-glycoside and its primary protective effect on HLECs[J].Chin JPharm(中国医药工业杂志),2015,46(11):1197-1201.
[9]LIU D.The expression and significance of early growth response-1 in selenite-induced cataract as well as in lens epithelia cells which are impaired by H_2O_2[D].Changsha:Central South University,2007.
[2]STINTXING FC,STINTZING AS,CARLE R,et al.Color and antioxidant properties of C3Ganidin-based anthoC3Ganin pigments[J].J Agric Food Chem,2002,50(21):6172-6181.
[3]MORIMISU Y,KUBOTA K,TASHIRO T,et al.Inhibitory effect of anthocyanins and colored rice on diabetic cataract formation in the rat lenses[J].Inter Congress Series,2002,1245:503-508.
[4]LUO QL,ZHAO JM,ZHANG XR,et al.Nanostructured lipid carrier(NLC)coated with chitosan oligosaccharides and its potential use in ocular drug delivery system[J].Int J Pharm,2011,403:185-191.
[5]SHRESTHA N,SHAHBAZI MA,ARAUJO F,et al.Chitosan-modified porous silicon microparticles for enhanced permeability of insulin across intestinal cell monolayers[J].Biomaterials,2014,35(25):7172-7179.
[6]WANG SL,ZHANG J,JIANG TY,et al.Protective effect of Coenzyme Qioagainst oxidative damage in human lens epithelial cells by novel ocular drug carriers[J].Int J Pharm,2011,403(1-2):219-229.
[7]LI X,LUO Y,LIAO ZG,et al.Effect of chitosan and its derivatives as coating materials on corneal permeability of the curcumin-loaded liposomes[J].Chin J Pharm(中国医药工业杂志),2015,46(3):265-269.
[8]ZHANG J,LUO YX,LIAO ZG,et al.Preparation of membrane-modified liposomes loaded with cyanidin-3-glycoside and its primary protective effect on HLECs[J].Chin JPharm(中国医药工业杂志),2015,46(11):1197-1201.
[9]LIU D.The expression and significance of early growth response-1 in selenite-induced cataract as well as in lens epithelia cells which are impaired by H_2O_2[D].Changsha:Central South University,2007.