内部萃取电喷雾电离质谱直接分析肺组织样品化学组分
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- 英文论文题名：Direct Characterization Chemicals of Lung Tissues Using Internal Extractive Electrospray Ionization Mass Spectrometry
- 论文作者：李云 ; 王志豪 ; 顾海巍 ; 陈焕文
- 英文论文作者：Yun Li ; Zhihao Wang ; Haiwei Gu ; Huanwen Chen ; Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation ; East China University of Technology
- 年：2016
- 作者机构：东华理工大学江西省质谱科学与仪器重点实验室;
- 论文关键词：内部萃取电喷雾电离质谱法 ; 直接分析 ; 组织样品 ; 氨基酸
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：O657.63;R563
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：206k
- 原文格式：D
- 会议级别：全国
摘要
离子化技术是近年来常压快速质谱分析技术的重点,基于不同离子化技术建立了许多各具特色的质谱分析方法。然而,直接分析样品内部化学组分的电离技术鲜有报道。内部萃取电喷雾电离(iEESI)~([1])能够直接对组织样品内部的化学物质进行萃取和离子化,结合质谱高灵敏度、高特异性的特点,实现定性和定量分析。本文以肺组织样品中的氨基酸为研究对象,在无需样品预处理的情况下使用iEESI-MS进行内部分析,并使用DESI-MS~([2])进行表面分析对比实验。实验结果表明,iEESI-MS能够快速、灵敏地检测到多种氨基酸,证明了该方法在全面获取组织样品信息方面的优势。另外,利用iEESI-MS对肺癌和癌旁组织进行分析,并结合主成分分析(PCA)快速、准确地区分了肺癌和癌旁组织。以上结果表明,iEESI-MS是研究生物组织内部成分的理想工具,并有望应用于临床诊断、生物学、代谢组学等领域。
iEESI-MS is characterized by extraction and ionization processes occur simultaneously,and can directly qualitative and quantitative analysis the interior chemicals of bulk sample,combining mass spectrometry with high sensitivity and high specificity.In this paper,bulk samples of lung tissue with amino acid were analyzed by iEESI-MS.Moreover,iEESI-MS is compared to DESI-MS proved that it can detect variety of amino acids of lung tissue quickly and sensitively.In the PCA score plot,lung cancer samples were clearly separate from the adjacent normal tissues that were analyzed by iEESI-MS.These findings suggest that it provides a powerful tool for studying internal chemicals of biological tissues,and offer possibilities applications in the field of metabolomics for cancer,medical engineering,and clinical diagnosis.
iEESI-MS is characterized by extraction and ionization processes occur simultaneously,and can directly qualitative and quantitative analysis the interior chemicals of bulk sample,combining mass spectrometry with high sensitivity and high specificity.In this paper,bulk samples of lung tissue with amino acid were analyzed by iEESI-MS.Moreover,iEESI-MS is compared to DESI-MS proved that it can detect variety of amino acids of lung tissue quickly and sensitively.In the PCA score plot,lung cancer samples were clearly separate from the adjacent normal tissues that were analyzed by iEESI-MS.These findings suggest that it provides a powerful tool for studying internal chemicals of biological tissues,and offer possibilities applications in the field of metabolomics for cancer,medical engineering,and clinical diagnosis.
引文
[1]Zhang,H.;Chingin,K.;Zhu,L.;Chen,H.Anal.Chem.,2015,87:2878-2883.
[2]Cooks,R.G.;Ouyang,Z.;Takats,Z.;Wiseman,J.M.Science,2006,311:1566-1570.
[2]Cooks,R.G.;Ouyang,Z.;Takats,Z.;Wiseman,J.M.Science,2006,311:1566-1570.