正交设计优化常山总碱的超声提取工艺
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- 英文论文题名：Utrasonic extraction process of total dichroa alkaloid were optimized by orthogonal design
- 论文作者：郭志廷 ; 王玲 ; 李春 ; 梁剑平 ; 尚若锋 ; 杨珍
- 英文论文作者：GUO Zhi-ting ; WANG Ling ; LI Chun ; LIANG Jian-ping ; SHANG Ruo-feng ; YANG Zhen ; Key Laboratory of Veterinary Pharmaceutics Discovery ; Ministry of Agriculture ; Key Laboratory of New Animal Drug Project ; Gansu Province ; Lanzhou Institute of husbandry and Pharmaceutical sciences of CAAS ; Institute of Chinese Materia Medica ; China Academy of Chinese Medical Sciences
- 年：2016
- 作者机构：中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室;中国中医科学院中药研究所;
- 论文关键词：正交设计 ; 常山总碱 ; 超声提取 ; 优化
- 英文论文关键词：orthogonal design ; total dichroa alkaloid ; ultrasound extraction ; optimization
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R284.2
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：4
- 文件大小：213k
- 原文格式：O
- 会议级别：全国
摘要
目的:确定常山总碱超声提取的最佳工艺条件。方法:采用稀盐酸溶媒、超声提取法从中药常山中分离常山总碱,应用正交试验法确定其最佳提取工艺条件,采用分光光度法测定常山碱含量。结果:常山总碱最佳提取工艺为:提取温度50℃,料液比1:5,提取时间1 h;上述条件下常山总碱的提取率为2.57%,其中常山碱的含量为0.30%。结论:超声提取法用于常山总碱的分离,具有省时、节能、提取率高等优点。
Objective To optimize ultrasonic extraction technology of total dichroa alkaloid(TDA).Methods:TDA of Dichroa febrifuga Lour were separated by dilute hydrochloric acid and ultrasonic extraction method.Its extraction process were optimized by orthogonal design.Dichroine content were determined by spectrophotometry.Results:The optimization process of TDA as follows:temperature 50℃,solid-liquid ratio 1:5,time 1 h.Under above conditions,TDA yield were up to2.57%) and the dichroine content were up to 0.30%.Conclusion:Isolation process of TDA is feasible by ultrasonic extraction method,which have advantages of time-saving,energy conservation and higher extraction rate.
Objective To optimize ultrasonic extraction technology of total dichroa alkaloid(TDA).Methods:TDA of Dichroa febrifuga Lour were separated by dilute hydrochloric acid and ultrasonic extraction method.Its extraction process were optimized by orthogonal design.Dichroine content were determined by spectrophotometry.Results:The optimization process of TDA as follows:temperature 50℃,solid-liquid ratio 1:5,time 1 h.Under above conditions,TDA yield were up to2.57%) and the dichroine content were up to 0.30%.Conclusion:Isolation process of TDA is feasible by ultrasonic extraction method,which have advantages of time-saving,energy conservation and higher extraction rate.
引文
1 LI Yan(李燕),LIU Ming-chuan(刘明川),JIN Lin-hong(金林红),et al.Advances on Chemical Components and Bioactivities of Dichroa febrifuga Lour(常山化学成分及生物活性研究进展).Guangzhou Chemical Industry(广州化工),2011,39(9):7
2 XU Hao-feng(徐浩锋),YANG Hua-jun(杨华俊),Yu Lan(俞岚),et al.Recent progress in the extraction methods for the active ingredients of Chinese herbal drugs(中药有效成分现代提取技术研究进展).Strait Pharmaceut ical Journa(海峡药学),2012,24(1):14
3 LI Zhi-ying(李志英),Lv Hong-yan(吕红艳),ZHANG Hai-rong(张海容),et al.Microwave extraction of artemisinin from Artemisia annua L(微波提取青蒿中青蒿素的研究).Chin J Pharm Anal(药物分析杂志),2010,30(8):1461
4 LIU Yong-cheng(刘永成),WANG Yi-bo(王一博),ZHANG Li-ying(张丽英),et al.Study by orthogonal design method on optimizing purification process for ossotide injection(正交设计法优选骨肽注射液纯化参数的研究).Chin J Pharm Anal(药物分析杂志),2011,31(4):683
5 LI Lu-xian(李禄先),QU Jia-wei(区嘉炜),XIE Shu-min(谢淑敏).Colorimetric estimation of febrifugine(常山碱的比色测定法).Acta Chimica Sinica(化学学报),1965,31(6):482
6 LIN Qing(林青),LI Lin-hai(李林海),ZHOU Hong-chao(周宏超),et al.Efficacy of recipe of traditional Chinese medicine on chickens infected with Eimeria maxima(中药复方制剂预防鸡巨型艾美耳球虫感染的效果观察).Chinese Journal of Veterinary Science(中国兽医学报),2011,31(2):194
7 LI Chun(李春),ZHANG Ya(张雅),LIN Li-mei(林丽美),et al.Quantitative Analysis of the febrifugine and isofebrifugine mixture in the root of Dichroa febrifuga lour(中药常山中常山碱和异常山碱的含量测定).Chin Pharm(中国药学杂志),2011,41(8):623
8 ZHAO Bing(赵兵),YIN Hui(尹辉).Circulation ultrasonic technology and its applications in the development and production of traditional Chinese medicine(循环超声技术及其在中药研发和生产中的应用).Chinese Traditional and Herbal Drugs(中草药),2006,37(1):154
2 XU Hao-feng(徐浩锋),YANG Hua-jun(杨华俊),Yu Lan(俞岚),et al.Recent progress in the extraction methods for the active ingredients of Chinese herbal drugs(中药有效成分现代提取技术研究进展).Strait Pharmaceut ical Journa(海峡药学),2012,24(1):14
3 LI Zhi-ying(李志英),Lv Hong-yan(吕红艳),ZHANG Hai-rong(张海容),et al.Microwave extraction of artemisinin from Artemisia annua L(微波提取青蒿中青蒿素的研究).Chin J Pharm Anal(药物分析杂志),2010,30(8):1461
4 LIU Yong-cheng(刘永成),WANG Yi-bo(王一博),ZHANG Li-ying(张丽英),et al.Study by orthogonal design method on optimizing purification process for ossotide injection(正交设计法优选骨肽注射液纯化参数的研究).Chin J Pharm Anal(药物分析杂志),2011,31(4):683
5 LI Lu-xian(李禄先),QU Jia-wei(区嘉炜),XIE Shu-min(谢淑敏).Colorimetric estimation of febrifugine(常山碱的比色测定法).Acta Chimica Sinica(化学学报),1965,31(6):482
6 LIN Qing(林青),LI Lin-hai(李林海),ZHOU Hong-chao(周宏超),et al.Efficacy of recipe of traditional Chinese medicine on chickens infected with Eimeria maxima(中药复方制剂预防鸡巨型艾美耳球虫感染的效果观察).Chinese Journal of Veterinary Science(中国兽医学报),2011,31(2):194
7 LI Chun(李春),ZHANG Ya(张雅),LIN Li-mei(林丽美),et al.Quantitative Analysis of the febrifugine and isofebrifugine mixture in the root of Dichroa febrifuga lour(中药常山中常山碱和异常山碱的含量测定).Chin Pharm(中国药学杂志),2011,41(8):623
8 ZHAO Bing(赵兵),YIN Hui(尹辉).Circulation ultrasonic technology and its applications in the development and production of traditional Chinese medicine(循环超声技术及其在中药研发和生产中的应用).Chinese Traditional and Herbal Drugs(中草药),2006,37(1):154