罗非鱼源无乳链球菌荚膜多糖合成基因的表达及与细菌毒力的关系分析
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- 英文论文题名：Relationship between the expression of capsular polysaccharide synthetic gene of Streptococcus agalactiae isolated from Nile tilapia and bacterial virulence
- 论文作者：师红亚 ; 董浚键 ; 叶星
- 英文论文作者：SHI Hongya ; DONG Junjian ; YE Xing ; Key Laboratory of Tropical&Subtropical Fisheries Resource Application&Cultivation of China Ministry of Agriculture ; Pearl River Fisheries Institute ; Chinese Academy of Fishery Sciences ; College of Fisheries and Life Science ; Shanghai Ocean University
- 年：2016
- 作者机构：农业部热带亚热带水产资源利用与养殖重点实验室珠江水产研究所中国水产科学研究院;上海海洋大学水产与生命技术学院;
- 论文关键词：尼罗罗非鱼 ; 无乳链球菌 ; 温度 ; 唾液酸 ; 荚膜多糖合成基因 ; 毒力
- 英文论文关键词：Oreochromis niloticus ; Streptococcus agalactiae ; temperature ; sialic acid ; capsular polysaccharide synthetic gene ; virulence
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S941.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
本实验克隆了罗非鱼源无乳链球菌荚膜多糖合成基因cpsE、cpsK和NeuA,并通过攻毒实验、唾液酸含量检测以及qPCR实验分析不同温度下此3个基因的表达与细菌荚膜唾液酸含量及细菌毒力相关性。Blast分析显示3个基因均具有一个糖基转移酶结构域。不同温度培养的无乳链球菌毒力与罗非鱼的死亡率呈正相关;在28和34℃条件下培养,细菌的毒力与cpsE、cpsK和NeuA的表达量及唾液酸的含量呈正相关,但在22℃时cpsE、cpsK和NeuA的表达量和唾液酸含量都较高,细菌毒力却最低。本研究结果提示cpsE、cpsK和NeuA的表达量及唾液酸含量可能并不是决定细菌毒力的关键因子,在较高水温条件下可能还与某些重要毒力相关基因的表达有关。本研究结果有助于进一步了解鱼源无乳链球菌的致病机制。
Capsular polysaccharide synthetic gene cpsE,cpsK and NeuA of Streptococcus agalactiae(GBS) from Nile tilapia(Oreochromis niloticus)were clonedin the study.The relationship between the expression level of capsular polysaccharide synthetic gene at different temperature and bacterial virulence was analysed by artificial challenge test,the dection of sialic acid content and qPCR. A glycosyl transferase domain was found in cpsE,cpsK and NeuA,respectively.The virulence of GBS cultivated at different temperature was positively corelated with the mortality of Nile tilapia challenged. Moreover,The virulence of GBS cultivated at28 and 34 ℃ was positively related to the expression levels of cpsE,cpsK and NeuA and sialic acid content.However, the high expression levels of cpsE,cpsK and NeuA,high sialic acid content and the lowest virulence of GBS were found at 22 ℃.The results suggested that the expression levels of cpsE,cpsK and NeuA and sialic acid content may not be key virulence factorsin GBS.
Capsular polysaccharide synthetic gene cpsE,cpsK and NeuA of Streptococcus agalactiae(GBS) from Nile tilapia(Oreochromis niloticus)were clonedin the study.The relationship between the expression level of capsular polysaccharide synthetic gene at different temperature and bacterial virulence was analysed by artificial challenge test,the dection of sialic acid content and qPCR. A glycosyl transferase domain was found in cpsE,cpsK and NeuA,respectively.The virulence of GBS cultivated at different temperature was positively corelated with the mortality of Nile tilapia challenged. Moreover,The virulence of GBS cultivated at28 and 34 ℃ was positively related to the expression levels of cpsE,cpsK and NeuA and sialic acid content.However, the high expression levels of cpsE,cpsK and NeuA,high sialic acid content and the lowest virulence of GBS were found at 22 ℃.The results suggested that the expression levels of cpsE,cpsK and NeuA and sialic acid content may not be key virulence factorsin GBS.
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