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硅酸钠对烟草青枯病的控病效果及烟叶膜脂过氧化的影响
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摘要
为明确硅肥对烟草青枯病的抗性效果及其作用机理,采用田间试验研究了烟草云烟87品种施用硅酸钠对青枯病发病率、病情指数和烟叶膜脂过氧化的影响。结果表明,施用硅酸钠可降低烟草青枯病的发病率和病情指数。烟科所试点烟苗移栽后90 d和100 d,硅酸钠处理的烟株发病率分别较不施用硅肥的对照(CK)下降13.14%和12.08%,病情指数分别下降23.34%和21.26%,与CK间差异均达到极显著水平。随着青枯病发病时间的延长和发病程度的提高,烟草叶片中H_2O_2和丙二醛(MDA)含量增加,细胞膜透性和K~+外渗量增加。施用硅酸钠可显著降低烟草H_2O_2累积和MDA含量,减轻青枯病胁迫对烟草叶片的膜伤害,同时显著降低烟草超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,提高过氧化氢酶(CAT)的活性。
To clarify the effects and mechanisms of silicate fertilizer on tobacco resistance against bacterial wilt disease,field experiments were carried out with sodium silicate(Na_2SiO_3) on the disease incidence,disease index and lipid membrane peroxidation of Yunyan87.The results showed that Na_2SiO_3 application reduced the disease incidence and disease index on tobacco bacterial wilt disease.Comparing with the control,the disease incidence of tobacco seedlings treated with Na_2SiO_3 on the 90 th and 100 th days after transplanting decreased by13.14%and 12.08%,and the disease index by 23.34%and 21.26%.The difference between the treated tobacco and the control was statistically significant.With the proceeding and depravation of bacterial wilt disease,the contents of H_2O_2 and malondialdehyde(MDA) in tobacco leaves as well as membrane permeability and K~+leakage increased.Na_2SiO_3 application significantly decreased the contents of H_2O_2 and MDA,alleviated the membrane damage of tobacco,improved catalase activity,by significandy reducing the activities of superoxide dismutase and peroxidase.
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