洛神花花萼多酚的提取及其抗氧化、酪氨酸酶抑制作用研究
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- 英文论文题名：Extraction of Polyphenols from Dry Calyx of Roselle(Hibiscus Sabdariffa L.) and Its Antioxidant,Tyrosinase Inhibition Properties
- 论文作者：章苏宁 ; 马冬雪 ; 张健
- 英文论文作者：ZHANG Su-ning ; MA Dong-xue ; ZHANG Jian ; School of Perfume and Aroma Technology ; Shanghai Institute of Technology
- 年：2016
- 作者机构：上海应用技术大学香料香精技术与工程学院;
- 论文关键词：洛神花 ; 多酚 ; 抗氧化能力 ; 酪氨酸酶抑制作用 ; 美白
- 英文论文关键词：Roselle ; Polyphenol ; Antioxidant capacity ; Tyrosinase Inhibition Properties ; Whitening effect
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ464
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：8
- 文件大小：439k
- 原文格式：O
- 会议级别：全国
摘要
采用超声波提取洛神花花萼中的多酚有效成分,并应用Folin-Ciocalteu法测定提取物中多酚的含量,比较水、乙醇、乙酸乙酯三种不同溶剂对多酚提取率的影响。选择乙醇为基本提取溶剂,以单因素试验为基础,进行正交试验对提取工艺优化,同时通过测定提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH)、羟自由基(·OH)和超氧阴离子自由基(O2-·)的清除能力来评价其酚类物质的抗氧化性能。结果表明:洛神花花萼在固定超声波功率为120W下的最佳提取工艺为提取温度45℃,料液比1:15,提取时间20min,乙醇体积分数75%。取正交试验中多酚得率为3.254%的样品做体外自由基清除实验,结果显示其抗氧化效果与抗氧化剂2,6-二叔丁基-4-甲基苯酚(BHT)相当。样品浓度为1mg/mL时,对三种自由基的清除率依次为96.8%、82.6%、62.5%。此外,文中还通过测定提取物对酪氨酸酶活性的抑制效果考察其美白性能。
Polyphenols were extracted from dried roselle calyx by ultrasonic technology and its content was determined using Folin-Ciocalteu method.Three different solvents(water,ethanol,ethyl acetate) were used to compare the extraction efficiencies of polyphenols.The ethanol solvent was chosen as the basic solvent for further study.By using single factor test and orthogonal test,the optimized ultrasonic extraction process of roselle dry calyx polyphenols was determined.Antioxidant effect of the extraction was measured by its clearance rate of diphenyl picryl hydrazinyl radical,hydroxyl radical and superoxide anion radical.The results showed that,the optimized extraction conditions were as follows:extraction temperature 45 ℃,solid-liquid ratio 1:15,extraction time 20 min,75%ethanol solvent.The antioxidant activity of the sample which polyphenols yields is 3.254%was similar to the value of BHT.The radical-scavenging activities were 96.8%、82.6%、 62.5%in turn when the concentration was 1mg/mL.In addition,the whitening effect of samples was also studies by determining the inhibition of tyrosinase activity.
Polyphenols were extracted from dried roselle calyx by ultrasonic technology and its content was determined using Folin-Ciocalteu method.Three different solvents(water,ethanol,ethyl acetate) were used to compare the extraction efficiencies of polyphenols.The ethanol solvent was chosen as the basic solvent for further study.By using single factor test and orthogonal test,the optimized ultrasonic extraction process of roselle dry calyx polyphenols was determined.Antioxidant effect of the extraction was measured by its clearance rate of diphenyl picryl hydrazinyl radical,hydroxyl radical and superoxide anion radical.The results showed that,the optimized extraction conditions were as follows:extraction temperature 45 ℃,solid-liquid ratio 1:15,extraction time 20 min,75%ethanol solvent.The antioxidant activity of the sample which polyphenols yields is 3.254%was similar to the value of BHT.The radical-scavenging activities were 96.8%、82.6%、 62.5%in turn when the concentration was 1mg/mL.In addition,the whitening effect of samples was also studies by determining the inhibition of tyrosinase activity.
引文
[1]王金亭.天然玫瑰茄红色素研究进展[J].粮食与油脂,2010(11):40-43.
[2]顾关云,蒋昱.玫瑰茄的化学成分与生物活性[J].现代药物与临床,2010,25(2):109-115.
[3]TSAI Pi-Jen,MCINTOSH John,PEARCE Philip,et al.Anthocyanin and antioxidant capacity in Roselle(Hibiscus sabdariff a L.)extract[J].Food Research International,2002,35:351-356.
[4]LIU Jer-Yuh,CHEN Chang-Che,WANG Wen-Hong,et al.The protective effects of Hibiscus sabdariffa extract on CCU-induced liver fibrosis in rats[J].Food Chem Toxicol,2006,44(3):336-343.
[5]董莎莎,宝福凯,吕青,等.玫瑰茄挥发油的GC-MS分析及其抗茵活性研究[J].大理学院学报,2009,8(6):1-4.
[6]LIU Jian-Li,YUAN Jiang-feng,ZHANG Zhi—qi.Microwave-assisted extraction optimised with response surface methodology and antioxidant activity of polyphenols from hawthorn(Crataegus pinnatifida Bge)fruit[J].International Journal of Food Science and Technology,2010,45(11):2400-2406.
[7]MATTEO Bonoli,VITO Verardo,EMANUELE Marconi,et al.Antioxidant phenols in barley(Hordeum vulgare L.)flour:comparative spectrophotometric study among extraction methods of free and bound phenolic compounds[J].Agricultural Food Chemistry,2004,(52):5195-5200.
[8]邓义书,包海荣,许慧.超声波辅助提取桑椹果渣中多酚物质的工艺研究[J].食品工业科技,2011,32(8):26-29.
[9]SUN Ting,HO Chi-Tang.Antioxidant activities of buckwheat extracts[J].Food Chemistry,2005,90(4):743-749.
[10]LIU Ben-guo,ZHAN Yu,NING Zheng-xiang,et al.Characterization and antioxidant activity of flavonoid extract from leaves of adinandra nitida Merr.es li[J].Chemistry and industry of forest products,2008,28(1):6-10.
[11]陶冉,王成章,孔振武.Sigmoidal拟合曲线法研究银杏叶类脂轻馏分的抗氧化性作用[J].林产化学与工业,2013,33(6):14-18.
[2]顾关云,蒋昱.玫瑰茄的化学成分与生物活性[J].现代药物与临床,2010,25(2):109-115.
[3]TSAI Pi-Jen,MCINTOSH John,PEARCE Philip,et al.Anthocyanin and antioxidant capacity in Roselle(Hibiscus sabdariff a L.)extract[J].Food Research International,2002,35:351-356.
[4]LIU Jer-Yuh,CHEN Chang-Che,WANG Wen-Hong,et al.The protective effects of Hibiscus sabdariffa extract on CCU-induced liver fibrosis in rats[J].Food Chem Toxicol,2006,44(3):336-343.
[5]董莎莎,宝福凯,吕青,等.玫瑰茄挥发油的GC-MS分析及其抗茵活性研究[J].大理学院学报,2009,8(6):1-4.
[6]LIU Jian-Li,YUAN Jiang-feng,ZHANG Zhi—qi.Microwave-assisted extraction optimised with response surface methodology and antioxidant activity of polyphenols from hawthorn(Crataegus pinnatifida Bge)fruit[J].International Journal of Food Science and Technology,2010,45(11):2400-2406.
[7]MATTEO Bonoli,VITO Verardo,EMANUELE Marconi,et al.Antioxidant phenols in barley(Hordeum vulgare L.)flour:comparative spectrophotometric study among extraction methods of free and bound phenolic compounds[J].Agricultural Food Chemistry,2004,(52):5195-5200.
[8]邓义书,包海荣,许慧.超声波辅助提取桑椹果渣中多酚物质的工艺研究[J].食品工业科技,2011,32(8):26-29.
[9]SUN Ting,HO Chi-Tang.Antioxidant activities of buckwheat extracts[J].Food Chemistry,2005,90(4):743-749.
[10]LIU Ben-guo,ZHAN Yu,NING Zheng-xiang,et al.Characterization and antioxidant activity of flavonoid extract from leaves of adinandra nitida Merr.es li[J].Chemistry and industry of forest products,2008,28(1):6-10.
[11]陶冉,王成章,孔振武.Sigmoidal拟合曲线法研究银杏叶类脂轻馏分的抗氧化性作用[J].林产化学与工业,2013,33(6):14-18.