长链非编码RNA编码的Pm-miR-133调控马氏珠母贝RhoA基因的表达
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- 英文论文题名：Pm-miR-133 hosting in one potential lncRNA regulates RhoA expressionin pearl oyster Pinctada fucata martensii
- 论文作者：郑哲 ; 黄荣莲 ; 田荣荣 ; 焦钰 ; 杜晓东
- 英文论文作者：ZHENG Zhe ; HUANG Ronglian ; TIAN Rongrong ; JIAO Yu ; DU Xiaodong ; Fisheries College ; Guangdong Ocean University
- 年：2016
- 作者机构：广东海洋大学水产学院;
- 论文关键词：LncRNA ; miR-133 ; RhoA ; 马氏珠母贝
- 英文论文关键词：LncRNA ; miR-133 ; RhoA ; Pinctada fucata martensii
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：560k
- 原文格式：D
- 会议级别：全国
摘要
在脊椎动物中,Mir-133调控心肌细胞和骨骼肌细胞的增殖和分化。本研究利用miR-RACE技术验证了pm-miR-133序列的准确性并通过stem-loop qRT-PCR检测其在闭壳肌、性腺、肝胰腺、外套膜、足和鳃中的表达模式。结果显示pm-miR-133在闭壳肌中的表达量显著高于其他组织(P<0.05)。RNAhybrid靶向分析发现pm-miR-133可以调控pm-RhoA的表达。本研究利用马氏珠母贝基因组序列进一步分析了pm-miR-133的前体RNA。结果显示,pm-miR-133前体precursor miR-133具有典型的颈环结构且与帽贝具有较高的同源性;该前体存在于长链非编码RNA Lnc133。Lnc133在闭壳肌、鳃、肝胰腺和性腺中均表达量较高。本研究利用precursor miR-133序列进行系统进化分析发现脊索动物和无脊索动物的miR-133属于两个不同的类群。综上所述,以Lnc133为宿主的miR-133可能通过靶向pm-RhoA参与调控闭壳肌的细胞增殖。
In vertebrates, miR-133 regulates the differentiation and proliferation of cardiac and skeletal muscles. In the present study, the precise sequence of mature pm-miR-133 was validated through miR-RACE. Stem loop qRT-PCR analysis demonstrated that mature pm-miR-133 was constitutively expressed inthe adductor muscle, gonad, hepatopancreas, mantle, foot, and gill of P.fucata martensii. Among these tissues, the adductor muscle exhibited the highest pm-miR-133 expression. Target analysis indicated that pm-RhoA was the potential regulatory target of pm-miR-133. Bioinformatics analyses revealed that a potential LncRNA(designated as Lnc133)with a mature pm-miR-133 could generate a hairpin structure that was highly homologous to that of Lottia gigantea. Lnc133 was also highly expressed in the adductor muscle, gill, hepatopancreas,and gonad. Phylogenetic analysis further showed that the miR-133 s derived from chordate and achordate were separated into twoclasses. Therefore, Lnc133 hosting pm-miR-133 could be involved in regulating the cell proliferation of adductor muscles by targeting pm-RhoA.
In vertebrates, miR-133 regulates the differentiation and proliferation of cardiac and skeletal muscles. In the present study, the precise sequence of mature pm-miR-133 was validated through miR-RACE. Stem loop qRT-PCR analysis demonstrated that mature pm-miR-133 was constitutively expressed inthe adductor muscle, gonad, hepatopancreas, mantle, foot, and gill of P.fucata martensii. Among these tissues, the adductor muscle exhibited the highest pm-miR-133 expression. Target analysis indicated that pm-RhoA was the potential regulatory target of pm-miR-133. Bioinformatics analyses revealed that a potential LncRNA(designated as Lnc133)with a mature pm-miR-133 could generate a hairpin structure that was highly homologous to that of Lottia gigantea. Lnc133 was also highly expressed in the adductor muscle, gill, hepatopancreas,and gonad. Phylogenetic analysis further showed that the miR-133 s derived from chordate and achordate were separated into twoclasses. Therefore, Lnc133 hosting pm-miR-133 could be involved in regulating the cell proliferation of adductor muscles by targeting pm-RhoA.
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