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15种植物精油对鲜切苹果抑菌性的研究
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摘要
本实验选用单增李斯特菌、金黄色葡萄球菌、大肠杆菌O157:H7以及鼠伤寒沙门氏菌四种食源性致病菌作为供试菌种,利用滤纸片法测定15种植物精油对目标菌的抑制能力。以鲜切苹果为实材料,探讨在相同贮藏条件下,经过不同浓度(0.05%、0.35%、0.65%)的百里香精油处理,对鲜切苹果上微生物生长的影响。结果表明,通过测量抑菌圈的直径大小,初筛出牛至精油、肉桂精油和百里香精油对供试菌种的抑菌作用较为显著。采用倍比稀释法测定这三种植物精油对供试菌种的最小抑菌浓度,结果表明百里香植物精油最小抑菌浓度较低。通过分析处理后鲜切苹果上菌落数量的变化趋势,确定百里香精油浓度为0.65%时抑菌效果最佳。本研究为植物精油能够进一步应用于鲜切果蔬产品中奠定了理论基础。
L.monocytogenes,S.aureus,E.coli 0157:H7 and Salmonella typhimurium were chosen as test strain in this study.The antibacterial activity of 15 essential was measured using filter paper method.Fresh-cut apple was chosen as experimental,materials to understand the effect of different concentrations(0.05%、0.35%、0.65%) of essential oils on microorganisms on sample under the same storage conditions.The result showed the inhibition ability of thyme essential oil,oregano essential oil,cinnamon essential oil were more significantly than other essential oils.The minimum inhibitory concentration of essential oils screened was determined for the corresponding pathogen using dilution method.It indicated thyme essential oil could significantly inhibit four pathogens.The change of microorganism population on fresh-cut apple was analyzed to understand the inhibitory concentration of essential oil during storage time.The result demonstrated that the antibacterial effect of thyme essential oil concentration as 0.65%is better than others.It provided theoretical foundation for investigate further the application of plant essential on fresh-cut fruits and vegetables products.
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