基于紫外光吸收法的DNA电位检测
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摘要
紫外吸收法是基于测定试样对紫外光的吸收来测定物质成分和含量的方法。聚合物膜离子选择性电极检测技术由于具有仪器简单、选择性好、分析速度快、检测成本低、易于实现微型化等优点,在医学诊断、环境监测、食品分析等领域具有广泛的应用前景。本实验中通过紫外吸收法,运用电化学DNA传感器原理,依据所加电活性指示剂与DNA单、双链作用的不同进行识别。它能快速检测特定DNA序列及突变,成本低,易于集成化和自动化。本论文中的一系列实验过程均属于探索性实验,对未来生物传感器的发展提供了重要信息。
引文
[1]付虎,易钢.基于核酸电化学适体生物传感器的研究进展,重庆医科大学检验医学院,国际检验医学杂志,2012,23(7):33-37.
[2]陶满兰,胡银香,谭琳,杨光明,杨云慧.适体生物传感器研究进展,云南化工,2008,35(6):46-51.
[3]游春苹,吴正钧,王荫榆,孔继烈,刘宝红,郭本恒.纳米材料在电化学生物传感器中的应用,化学传感器,2009,29(3):1-7.
[4]程圭芳,黄翠华,赵洁,谭雪莲,任品刚.方禹之新型磁性纳米电化学DNA生物传感器的研究,分析化学研究报告,2009,37(2):169-73.
[5]伍齐.核酸适体用于肿瘤细胞的检测与富集研究,湖南大学生物学院,2011,研究生毕业论文.
[6]卢建华.生物传感器的应用与发展趋势,科学导报,2012,6(2):326-327.
[7]马莉萍,毛斌,刘斌,李工农,韩根亮,刘国汉.生物传感器的应用现状与发展趋势,传感器与微系统,2009,52(9):7-11.
[8]刘俊芳,李彦青,郭满栋.电化学DNA生物传感器的研究现状,山西师范大学,化学系,2007,研究生毕业论文.
[9]Hibbert D B.The analytical division of IUPAC[J].Trac-Trends Anal Chem.2005,24(4):281-284
[10]Arnold M A.Meyerhoff M E.Recent advances in the development and analytical applications of biosensing probes[J],Crc Critical Reviews in Analytical Chemistry,1998,20(3):149-196.
[11]Ma S C,Yang V C,Meyerheoff M E.Heparin-responsive electrochemical sensor-a preliminary-study[J].Analytical Chemistry,1992,64(6):694-697.
[12]Ramamurthy N,Baliga N,Wahr J A,et al.Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration[J].Clinical Chemistry,1998,44(3):606-613.
[13]Ma S C,Yang V C,Meyerhoff M E,Heparin-responsive electrochemical sensor-a preliminary study[J].Anal Chem,1992,64(6):694-697.
[14]Potentiometric Urea Biosensor Based on Ion-Selective Electrode by Different Immobilization Procedures,Rare metal materials and engineering,2006,35(3),309-312
[15]Yun J H,Meyerhoff M E,Yang V C.Protamine-sensitive polymer membrane electrode:characterization and bioanalytical applications,Anal Chem.1995,224:212-220.
[16]Buhlmann,P.Pretsch,E.Carrier-based ion-selective electrodes and bulk optodes.1.General characteristic[J].Chem.Rev,1997,97(8):3083-3132.
[17]Palecek E.Adsorptive transfer stripping voltammetry:Detection of nanogram quantities of DNA immobilized at the electrodes surface[J].Anal biochem,1988(170):421-431.
[18]Wang J,Cai X H,Jonsson C,et al.Adsorptive stripping potentiometry of DNA at electrochemically pretreatecarbon paste electrodes[J].Electroanalysis,1996,(8):20-24.
[19]Bakker E,Pretsch E.Modern Potentiometry[J].Angew.Chem.Int.Ed.Engl.2007,46(30):5660-5668.
[2]陶满兰,胡银香,谭琳,杨光明,杨云慧.适体生物传感器研究进展,云南化工,2008,35(6):46-51.
[3]游春苹,吴正钧,王荫榆,孔继烈,刘宝红,郭本恒.纳米材料在电化学生物传感器中的应用,化学传感器,2009,29(3):1-7.
[4]程圭芳,黄翠华,赵洁,谭雪莲,任品刚.方禹之新型磁性纳米电化学DNA生物传感器的研究,分析化学研究报告,2009,37(2):169-73.
[5]伍齐.核酸适体用于肿瘤细胞的检测与富集研究,湖南大学生物学院,2011,研究生毕业论文.
[6]卢建华.生物传感器的应用与发展趋势,科学导报,2012,6(2):326-327.
[7]马莉萍,毛斌,刘斌,李工农,韩根亮,刘国汉.生物传感器的应用现状与发展趋势,传感器与微系统,2009,52(9):7-11.
[8]刘俊芳,李彦青,郭满栋.电化学DNA生物传感器的研究现状,山西师范大学,化学系,2007,研究生毕业论文.
[9]Hibbert D B.The analytical division of IUPAC[J].Trac-Trends Anal Chem.2005,24(4):281-284
[10]Arnold M A.Meyerhoff M E.Recent advances in the development and analytical applications of biosensing probes[J],Crc Critical Reviews in Analytical Chemistry,1998,20(3):149-196.
[11]Ma S C,Yang V C,Meyerheoff M E.Heparin-responsive electrochemical sensor-a preliminary-study[J].Analytical Chemistry,1992,64(6):694-697.
[12]Ramamurthy N,Baliga N,Wahr J A,et al.Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration[J].Clinical Chemistry,1998,44(3):606-613.
[13]Ma S C,Yang V C,Meyerhoff M E,Heparin-responsive electrochemical sensor-a preliminary study[J].Anal Chem,1992,64(6):694-697.
[14]Potentiometric Urea Biosensor Based on Ion-Selective Electrode by Different Immobilization Procedures,Rare metal materials and engineering,2006,35(3),309-312
[15]Yun J H,Meyerhoff M E,Yang V C.Protamine-sensitive polymer membrane electrode:characterization and bioanalytical applications,Anal Chem.1995,224:212-220.
[16]Buhlmann,P.Pretsch,E.Carrier-based ion-selective electrodes and bulk optodes.1.General characteristic[J].Chem.Rev,1997,97(8):3083-3132.
[17]Palecek E.Adsorptive transfer stripping voltammetry:Detection of nanogram quantities of DNA immobilized at the electrodes surface[J].Anal biochem,1988(170):421-431.
[18]Wang J,Cai X H,Jonsson C,et al.Adsorptive stripping potentiometry of DNA at electrochemically pretreatecarbon paste electrodes[J].Electroanalysis,1996,(8):20-24.
[19]Bakker E,Pretsch E.Modern Potentiometry[J].Angew.Chem.Int.Ed.Engl.2007,46(30):5660-5668.