益生菌免疫调节功能研究

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• 英文论文题名：Immunomodulatory effects of probiotics
• 论文作者：伍梓汐 ; 李姗珊 ; 沈曦 ; 李鸣 ; 崔文静 ; 张其圣 ; 何方
• 英文论文作者：Wu Zixi ; Li Shanshan ; Shen Xi ; Li Ming ; Cui Wenjing ; Zhang Qisheng ; He Fang ; West China College of Public Health ; Sichuan University ; Hebei ; Inatural Biological Technology Co.Ltd. ; Sichuan Food Fermentation Industry Research and Sign Institute
• 年：2016
• 作者机构：四川大学华西公共卫生学院;河北一然生物科技有限公司;四川省食品发酵工业研究设计院;
• 论文关键词：益生菌 ; 免疫调节 ; 过敏
• 英文论文关键词：probiotics ; immunoregulation ; allergy
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：TS201.3
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：110k
• 原文格式：O
• 会议级别：全国

摘要

目的使用小鼠巨噬细胞和致敏模型小鼠探讨乳酸杆菌及双歧杆菌的菌株特异性免疫调节及抗过敏作用的可能性。方法使用Lactobacillus acidophilus 6091(6091)、L.plantarum R-1-1(R-1-1)与小鼠巨噬细胞样细胞系J774A.1细胞(J774A.1细胞)进行共培养,用酶联免疫吸附测定法测定共培养上清液白细胞介素-6,10(Interleukin-6,10,IL-6,10)。使用R-1-1、6091、Bifidobacterium bifidum TMC3115(TMC3115)菌体对受试小鼠进行连续灌胃持续5周,在7、21、28天分别注射卵清白蛋白(OVA)和氢氧化铝诱导其产生过敏反应,采用ELISA试剂盒检测血液总IgE,同时用实时荧光定量PCR法检测脾脏细胞的IL-6、IL-12、TNF-α关于mRNA的表达。结果各受试菌株有意地诱导J774A.1细胞产生IL-10、IL-6(P<0.05)。各受试菌株灌胃小鼠血液IgE与OVA阳性对照组比较显著地降低(P<0.05);同时,各受试菌株灌胃小鼠脾脏细胞的IL-6、IL-12、TNF-α和OVA阳性对照组比较显著地降低(P<0.05);结论 6091、R-1-1、TMC3115能有效地通过活化巨噬细胞调节机体的免疫调节作用,降低血液IgE产生,其作用机制可能与诱导IL-10的分泌,进而同时下调了TH1和TH2免疫应答有关。
Objective:This study aims to test the possibilitiesof Lactobacilli and Bifidobacteria against allergy by regulating the immunity of the host animal.Methods:Murine macrophage-like cell line J774 A.1 were co-cultured with the cells of Lactobacillus acidophilus 6091(6091),L.plantarumR-1-1(R-1-1).The supernatant of the J774 A.1 cell culture were collected for detecting production of interleukin-6(IL-6) and IL-10 usingELISA.The male BALB/Cmice were orally administrated with the active cells of 6091,R-1-1 and Bifidobacterium bifidum TMC3115(TMC3115) for 5 weeks and were sensitizedwith ovalbumin(OVA) at day 7,21 and 28.The serum total IgE ofthe tested mice wasanalyzed with ELISA before and after the intervention.The expression of spleen IL-6,IL-12 and TNF-α mRNA in the tested mice were also evaluated with Quantitative Real-time PCR.Results:Each of tested bacteriasignificantlystimulated J774 A.1 cells to produce IL-6 and 10 in a strain dependent manner(P < 0.05).The increased of serum IgE of the tested mice were also decreased significantly by the treatment of three bacteria(P < 0.05).The expression of the spleen IL-6,IL-12,TNF-a mRNA of the tested mice was also significantly suppressed by the orally fed bacteria(P < 0.05).Conclusion:These results indicate that lactobacilli and bifidobacteria could inhibit IgE mediated allergic disorder by suppressing the serum IgE production caused by food allergens.One of the possible underlying mechanism might be their abilities of these bacteria to stimulate IL-10 production by which to down-regulate the hyper-immune responses including both Th1 and Th2 types.

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