碳化硅量子点荧光特性及其致病镰刀菌活体细胞标记机制
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- 英文论文题名：Fluorescence characteristics and labeling mechanism for living cells of pathogenic fusarium with silicon carbide quantum dots
- 论文作者：柳洪洁 ; 孙丰飞 ; 宋月鹏 ; 朱彦敏 ; 康杰 ; Kim Hyoungseop
- 英文论文作者：LIU Hong-jie ; SUN Feng-fei ; SONG Yue-peng ; ZHU Yan-min ; KANG Jie ; KIM Hyoung-seop ; Shandong Agricultural University ; College of animal science and Veterinary Medicine ; Shandong Agricultural University ; Mechanical and Electronic Engineering College ; Pohang University of Science and Technology ; Department of Materials Science and Engineering
- 年：2016
- 作者机构：山东农业大学动物科技学院;山东农业大学机械与电子工程学院;韩国浦项工科大学;
- 论文关键词：碳化硅量子点 ; 致病镰刀菌 ; 活体细胞 ; 荧光特性与标记
- 英文论文关键词：silicon carbide quantum dots(SiC-QDs) ; pathogenic fusarium ; living cells ; fluorescence characteristics and labeling
- 会议召开时间：2016-08-16
- 会议录名称：第十届全国材料科学与图像科技学术会议暨校地企产学研合作创新论坛论文摘要集
- 语种：中文
- 分类号：Q6-3
- 学会代码：EGTA
- 会议名称：第十届全国材料科学与图像科技学术会议暨校地企产学研合作创新论坛
- 会议地点：中国河南洛阳
- 主办单位：中国体视学学会材料科学分会、中国金属学会材料科学分会
- 学会名称：中国体视学学会
- 页数：7
- 文件大小：3633k
- 原文格式：O
- 会议级别：全国
摘要
研究了腐蚀法制各的碳化硅量子点荧光特性及其致病镰刀菌活体细胞标记强度与机制。结果表明当激发光为320nm时SiC量子点光致发光强度最大,随着激发光波长增加,发射光波长出现红移现象,且具有较高的斯托克斯位移,由于荧光发射可以全色调谐,从而实现了致病镰刀菌的近紫外荧光检测,可用来对自发荧光细胞的有效检测与定量分析。进一步对致病镰刀菌活体细胞SiC量子点荧光标记机制的研究结果表明,量子点通过网格蛋白依赖的内吞方式进入活体细胞内部,并均匀分布,从而实现了对致病镰刀菌的稳定荧光标记。另外基于实验结果与理论分析,提出了致病镰刀菌SiC量子点荧光标记模型。
Fluorescence characteristics of silicon carbide quantum dots(SiC-QDs) prepared by chemical corrosion method and labeling mechanism for the living cells of pathogenic fusarium were explored.The results indicated that SiC-QDs photoluminescence intensity reached the maximum value when excitation wavelength of 320 nm.Red shift phenomenon of emission wavelength will occur with the excitation wavelength increasing.Because of larger Stokes shift and tunable color fluorescent,near ultraviolet detection for living cells with SiC-QDs fluorescent marking was achieved,which can effectively detect and quantitative analysis the autofluorescence cells.The results of labeling mechanism for living cells of pathogenic fusarium with SiC-QDs exhibited that quantum dots can reach the cells interior and homogeneous distribution through the clathrin endocytsis action.The living cells were so stably fluorescent labeling.Moreover,marking model for living cells with SiC-QDs was established based on the experimental results and theoretical analysis.
Fluorescence characteristics of silicon carbide quantum dots(SiC-QDs) prepared by chemical corrosion method and labeling mechanism for the living cells of pathogenic fusarium were explored.The results indicated that SiC-QDs photoluminescence intensity reached the maximum value when excitation wavelength of 320 nm.Red shift phenomenon of emission wavelength will occur with the excitation wavelength increasing.Because of larger Stokes shift and tunable color fluorescent,near ultraviolet detection for living cells with SiC-QDs fluorescent marking was achieved,which can effectively detect and quantitative analysis the autofluorescence cells.The results of labeling mechanism for living cells of pathogenic fusarium with SiC-QDs exhibited that quantum dots can reach the cells interior and homogeneous distribution through the clathrin endocytsis action.The living cells were so stably fluorescent labeling.Moreover,marking model for living cells with SiC-QDs was established based on the experimental results and theoretical analysis.
引文
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[2]Pittet M J,Weissleder R W.Intravital Imaging[J].Cell,2011,147(5)983-991.
[3]Pan H,Zhang P F,Gao D Y,et al.Noninasive visualization of respiratory viral infection using bioorthogonal conjugated near-infrared-emitting quantum dots[J].ACS Nano,2014,8(6):5466-5477..
[4]Xu C J,Mu L Y,Roes I,et al.Nanoparticle-based monitoring of cell therapy[J].Nanotechnology,2011,22(49):494001.
[5]Medintz IL,Uyeda H T,Goldman E R,et al.Quantum dot bioconjugates for imaging,labeling and sensing[J].Nature,2005,4(6):435-446.
[6]Derfus A M,Chan W C W,Bhatia A N.Probing the cytotoxicity of semiconductor quantum dots[J].Nano Letters,2004,4(1):11-18.
[7]Fujimoto J G,Farkas D L.Biomedical Optical Imaging[M].Oxford:Oxford University Press,2009
[8]Chen N,He Y,Su Y Y,et al.The cytotoxicity of cadmium-based quantum dots[J].Biomaterials,2012,33(5):1238-1244.
[9]Beke D,Szekrenyes Z,Balogh I,et al.Preparation of small silicon carbide quantum dots by wet chemical etching[J].Journal of Materials Research,2013,28(1):44-49.
[10]Botsoa J,Lysenko V,Geloen A,et al.Application of 3C-SiC quantum dots for living cell imaging[J].Applied Physics Letters,92(2008):173902.
[11]Fan J Y,Li H X,Jiang J,et al.3C-SiC nanocrystals as fluerescent biological labels[J].Small,2008,4(8):1058-2062.
[12]Saddow S E.Silicon Carbide Biotechnology:A Biocompatible Semiconductor for Advanced Biomedical Devices and Applications[M].Elsevier's Science and Technology Rights Department in Oxford,UK.2012.
[13]Pourchez J,Forest V,Boumahdi N,et al.In vitro cellular responses to silicon carbide nanoparticles:impact of physico-chemical features on pro-inflammatory and pro-oxidative effects[J].Journal of Nanoparticle research,10(2012):1143-1173.
[14]宋月鹏,康杰,高东升,等.尖孢镰刀菌碳化硅量子点标记及其长时程荧光成像[J]农业工程学报,2013,29(17):286-292Song Y P,Kang J,Gao D S,et al.Labeling Fusarium oxysporum with silicon carbide quantum dots and long-term-distance fluorescent imaging for living cells[J].Transactions of the Chinese Society of Agricultural Engineering,2013,29(17):286-292.
[15]朱彦敏,宋月鹏,柳洪洁,等碳化硅量子点荧光标记串珠镰刀菌及其活体示踪[J],硅酸盐通报,2016,55(5):1596-1061.Zhu Y M,Song Y P,Liu H J,et al.Fluorescence Labeling and Tracing in Vivo for the Living Cells of Fusarium Moniliforme with Silicon Carbide Quantum Dots[J].Bulletin of the Chinese Ceramic Society,2016,55(5):1596-1061.
[16]宋月鹏,朱彦敏,康杰,等碳化硅量子点研究现状及其生物学应用[J]中国陶瓷,2014,50(7):5-9Song Y P,Kang J,Zhu Y M,et al.The current research status and application in biology of SiC quantum dots[J].China Ceramics,2014,50(7):5-9.
[17]Wang J,Xiong S J,Wu X L,et al.Glycerol-bonded 3C-SiC nanocrystal solid films exhibiting broad and stable violet to blue-green emission[J],Nano Letters,2010,(10):1466-1471.
[18]郭俊宏.碳化硅及金纳米颗粒表面结构诱导的荧光特性及其应用[D].南京:南京大学,2014Guo J H.Surface structure induced fluorescence characteristics of 3C-SiC and Au nanoparticles and its application[D],Ph.D thesis,Nanjing University,2014.
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