靶向整合素α_vβ_3的复合碳纳米点的诊疗一体化应用
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- 英文论文题名：Hybrid Integrin α_vβ_3-Targeted Carbon Nanodots and Their Theranostic Applications
- 论文作者：刘哲 ; 徐齐恩 ; 宋远会 ; 李宜鸿
- 英文论文作者：Zhe Liu ; Qien Xu ; Yuanhui Song ; Yihong Li ; Wenzhou Institute of Biomaterials and Engineering ; Chinese Academy of Sciences ; Wenzhou Institut of Biomaterials and Engineering ; Wenzhou Medical University
- 年：2016
- 作者机构：中科院温州生物材料与工程研究所(筹);温州生物材料与工程研究所,温州医科大学;
- 论文关键词：碳纳米点 ; 诊疗一体化 ; 整合素αvβ3 ; 乳腺癌
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：TB383.1;TQ421.7
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：324k
- 原文格式：D
- 会议级别：全国
摘要
应用靶向纳米材料构建兼具活体成像、药物缓控释功能的诊疗一体化试剂,在病灶部位定向聚集并通过光热作用杀伤肿瘤细胞,控制病灶发展直至消除病变组织,已成为纳米技术与精准医学交叉发展的热点研究方向。我们用碳基材料构建了碳纳米点并通过表面配体修饰实现了靶向整合素α_vβ_3受体,经由透射电镜、红外、XPS等手段对复合碳纳米点进行了化学表征。同时,经激光照射一定时间,复合碳纳米点溶液升温效果明显,温度变化对照射时间、激光功率与碳纳米点浓度均呈现依赖性。以一定浓度碳纳米点和乳腺癌细胞MDA-MB231共培养,激光共聚焦显微镜观察得到对细胞靶向效果明显。细胞毒性实验证实,复合碳纳米点对细胞有良好的生物相容性。因此,这一复合碳纳米点体系有望作为诊疗一体化试剂在活体成像、影像辅助治疗等方面得到更广泛的应用,从而为乳腺癌等疾病的早期诊疗提供新的策略与方法。
It becomes robust to develop theranostic nanoprobes for bio-imaging and targeting drug delivery and photothermal treatment(PTT) especially in the trans-disciplinary fields of nanotechnology and precision medicine. Herein, we report the fabrication of hybrid carbon nanodots(HCDs) and specific targeting to integrin α_vβ_3 for early diagnosis of breast cancers. The chemical characterization has been performed via TEM, FT-IR and XPS. The temperature change(ΔT) is closely correlated with exposure time, laser power and HCDs concentration. The cell-targeting capability has been verified by co-incubating HCDs with MDA-MB231 cells and visualized on a confocal laser scanning microscope. In the meanwhile, cell viability tests confirmed their good biocompatibility. Therefore, the developed hybrid carbon nanodots are promising theranostic candidates for bio-imaging and image-guided therapy. They will unprecedentedly provide new strategies for early detection and effective treatment of breast cancers.
It becomes robust to develop theranostic nanoprobes for bio-imaging and targeting drug delivery and photothermal treatment(PTT) especially in the trans-disciplinary fields of nanotechnology and precision medicine. Herein, we report the fabrication of hybrid carbon nanodots(HCDs) and specific targeting to integrin α_vβ_3 for early diagnosis of breast cancers. The chemical characterization has been performed via TEM, FT-IR and XPS. The temperature change(ΔT) is closely correlated with exposure time, laser power and HCDs concentration. The cell-targeting capability has been verified by co-incubating HCDs with MDA-MB231 cells and visualized on a confocal laser scanning microscope. In the meanwhile, cell viability tests confirmed their good biocompatibility. Therefore, the developed hybrid carbon nanodots are promising theranostic candidates for bio-imaging and image-guided therapy. They will unprecedentedly provide new strategies for early detection and effective treatment of breast cancers.
引文
[1]Song Y.;Shi W.;Chen W.;Li X.;Ma H.J.Mater.Chem.2012,22:12568.
[2]Mewada A.;Pandey S.;Thakur M.;Jadhav D.;Sharon M.J.Mater.Chem.B.2014,2:698.
[2]Mewada A.;Pandey S.;Thakur M.;Jadhav D.;Sharon M.J.Mater.Chem.B.2014,2:698.