基于荧光纳米簇和金属离子作用的生物分析方法
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- 英文论文题名：Development of fluorescent assay for biomolecules based on the interaction between noble metal nanoclusters and metal ions
- 论文作者：孙健 ; 杨秀荣
- 英文论文作者：Jian Sun ; Xiurong Yang ; State Key Laboratory of Electroanalytical Chemistry ; Changchun Institute of Applied Chemistry ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院长春应用化学研究所电分析化学国家重点实验室;
- 论文关键词：纳米簇 ; 荧光 ; 金属离子 ; 生物分析 ; 磷酸酶
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：Q-33
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：145k
- 原文格式：D
- 会议级别：全国
摘要
特异性靶标生物分子的分析检测往往与疾病的诊断和治疗息息相关,是生物医学和分析化学的重要研究内容之一。其中,荧光分析技术具有分析速度快,信号稳定,灵敏度高和选择性好等优点,一直在生命分析中占据着重要的位置。由几个到几百个原子组成的荧光贵金属纳米簇(金、银等)具有尺寸较小、制备过程简单、生物相容性好、光学性能优异等特点,是一种近年来新兴的荧光纳米材料,在生物分析与生物成像等领域有着很好的应用前景[1]。我们发展了一种水相的一锅合成方法,以氯金酸、硝酸银等为主要起始原料,利用多种不同的还原剂和小分子巯基配体,制备了几种不同性质的荧光金纳米簇(AuN Cs)及银金双金属纳米簇(AgAuNCs)。利用这些纳米簇的荧光对外界微环境变化响应敏感的特点,构建了金属离子的识别体系,并且在此基础上,实现了对焦磷酸酶[2]、半胱氨酸/组氨酸[3]、乙酰胆碱酯酶[4]、酸性磷酸酶[5]等生物分子的选择性灵敏检测。
Fluorescent assay is well-known one of the most efficient and accessible strategies that have been used in biomolecular recognition and biomedical diagnosis. As a promising kind of fluorophores, the fluorescent noble metal nanoclusters(NCs, typically Au and Ag) have recently been developed and applied in biosensing and bioimaging due to their ultrasmall size, good biocompatibility, and obvious intrinsic fluorescence. By means of the selective binding of certain metal ions(e.g. Cu~(2+) and Fe~(3+)), the fluorescent NCs have been used in the establishment of the novel and convenient assays for the biologically relevant molecules, such as the amino acids, acetylcholinesterase, inorganic pyrophosphatase, acid phosphatase and so on).
Fluorescent assay is well-known one of the most efficient and accessible strategies that have been used in biomolecular recognition and biomedical diagnosis. As a promising kind of fluorophores, the fluorescent noble metal nanoclusters(NCs, typically Au and Ag) have recently been developed and applied in biosensing and bioimaging due to their ultrasmall size, good biocompatibility, and obvious intrinsic fluorescence. By means of the selective binding of certain metal ions(e.g. Cu~(2+) and Fe~(3+)), the fluorescent NCs have been used in the establishment of the novel and convenient assays for the biologically relevant molecules, such as the amino acids, acetylcholinesterase, inorganic pyrophosphatase, acid phosphatase and so on).
引文
[1]Sun,J.et al.J.Mater.Chem.C,2013,1:138;2013,1:908;2014,2:8000;Nanoscale,2014,6:5449.
[2]Sun,J.;Yang,F.;Zhao,D.;Yang,X.R.Anal.Chem.,2014,86:7883.
[3]Sun,J.;Yang,F.;Zhao,D.;Chen,C.X.;Yang,X.R.ACS Appl.Mater.Interfaces,2015,7:6860.
[4]Sun,J.;Yang,X.R.Biosens.Bioelectron,2015,74:177.
[5]Sun,J.,Yang,F.,Yang,X.R.Nanoscale,2015,7:16372.
[2]Sun,J.;Yang,F.;Zhao,D.;Yang,X.R.Anal.Chem.,2014,86:7883.
[3]Sun,J.;Yang,F.;Zhao,D.;Chen,C.X.;Yang,X.R.ACS Appl.Mater.Interfaces,2015,7:6860.
[4]Sun,J.;Yang,X.R.Biosens.Bioelectron,2015,74:177.
[5]Sun,J.,Yang,F.,Yang,X.R.Nanoscale,2015,7:16372.