池塘养殖和海上吊笼养殖仿刺参肠道菌群结构对比分析
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- 英文论文题名：Comparative analysis of intestinal microflorasea cucumber cultivatedin ponds and raft cages
- 论文作者：陆振 ; 杨求华 ; 林琪 ; 黄瑞芳 ; 何丽斌 ; 葛辉 ; 周宸 ; 杜虹
- 英文论文作者：LU Zhen ; YANG Qiu-hua ; HUANG Rui-fang ; LIN Qi ; HE Li-bin ; GE Hui ; CHOU Chen ; DU Hong ; Department of Biology ; Shantou University ; Fisheries Research Institute of Fujian Province ; Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian
- 年：2016
- 作者机构：汕头大学生物系;福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室;
- 论文关键词：仿刺参 ; 16S rDNA ; 肠道菌群结构 ; 池塘养殖 ; 海上吊笼养殖
- 英文论文关键词：Apostichopus japonicus ; 16S rDNA ; intestinal microflora ; Ponds ; suspension cage
- 会议召开时间：2016-11-11
- 会议录名称：福建省海洋学会2016年学术年会论文摘要集
- 语种：中文
- 分类号：S968.9
- 学会代码：FJHY
- 会议名称：福建省海洋学会2016年学术年会
- 会议地点：中国福建厦门
- 主办单位：福建省海洋学会、福建省水产研究所
- 学会名称：福建省海洋学会
- 页数：1
- 文件大小：63k
- 原文格式：O
- 会议级别：地方
摘要
实验采用平板分离及16S rDNA序列分析法研究池塘养殖和海上吊笼养殖仿刺参(Apostichopus japonicus)的前肠、中肠和后肠菌群结构并进行对比分析。结果显示,在海上吊笼养殖的仿刺参的肠道内分离鉴定的240株菌株分属于三个门、11个属和34个种。在池塘养殖仿刺参的肠道内分离鉴定的211株菌株分属于三个门、11个属和49个种。两种养殖模式仿刺参肠道菌群结构存在较大差异,且同一仿刺参肠道各部分之间的菌群结构也存在较大的差异。在海上养殖仿刺参的肠道优势菌群为弧菌属(Vibrio)、Formosa、希瓦氏菌属(Shewanella),在池塘养仿刺参的肠道内优势菌群为弧菌属(Vibrio)、茅孢杆菌属(Bacillus)、喜盐茅孢杆菌属(Halobacillus)。两个菌群共有菌株有7个种存在于在不同的肠道部位。两种养殖模式比较发现池塘养仿刺参的肠道菌群的丰富度大于海上吊笼养殖的仿刺参,且在池塘养殖仿刺参肠道内茅孢杆菌(Bacillus)等益生菌的占比较高,海上吊笼养殖仿刺参分离得到较多的弧菌属(Vibrio)属细菌。研究可为南方仿刺参人工养殖中潜在益生菌的筛选提供基础资料。
The intestinal flora is essential in shaping many of its host's functional attributes.The microbial community structure in gut,including anterior intestine,middle intestine and posterior intestine of sea cucumber(Apostichopus japonicus),was studied by 16 S rDNA sequence analysis.The results showed that 240 strains were identified from A.japonicus in suspension cage closely related to 3 Phylum,11 genera and 34 different species.211 strains were identified from that in ponds closely related to 3 Phylum,11 genera and 49 different species.There were significant different structure of flora between each part of intestinal,and microflora populations of the entire gut between A.japonicus of ponds and suspension cage were quite different.Vibrio Formosa and Shewanella were predominant flora in suspension cage's gut.VibrioBacillus and Halophilous were predominant flora in ponds' gut.There were7 bacteria,which not only existed in the intestinal of ponds but also existed in the gut of suspension cage,but they dwelled in different parts of intestines.The species abundance in gut of ponds were significantly higher than those in suspension cage,and the number of Bacillus and other probiotics were also higher.
The intestinal flora is essential in shaping many of its host's functional attributes.The microbial community structure in gut,including anterior intestine,middle intestine and posterior intestine of sea cucumber(Apostichopus japonicus),was studied by 16 S rDNA sequence analysis.The results showed that 240 strains were identified from A.japonicus in suspension cage closely related to 3 Phylum,11 genera and 34 different species.211 strains were identified from that in ponds closely related to 3 Phylum,11 genera and 49 different species.There were significant different structure of flora between each part of intestinal,and microflora populations of the entire gut between A.japonicus of ponds and suspension cage were quite different.Vibrio Formosa and Shewanella were predominant flora in suspension cage's gut.VibrioBacillus and Halophilous were predominant flora in ponds' gut.There were7 bacteria,which not only existed in the intestinal of ponds but also existed in the gut of suspension cage,but they dwelled in different parts of intestines.The species abundance in gut of ponds were significantly higher than those in suspension cage,and the number of Bacillus and other probiotics were also higher.
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