蜡样芽孢杆菌生物膜对凡纳滨对虾生长及抗病力的影响
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- 英文论文题名：Effect of dietary with Bacillus cereus biofilm on the growth and disease resistance of Litopenaeus vannamei
- 论文作者：宋晓玲 ; 许华 ; 刘文亮 ; 唐杨
- 英文论文作者：SONG Xiaoling ; XU Hua ; LIU Wenliang ; TANG Yang ; Yellow Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences
- 年：2016
- 作者机构：中国水产科学研究院黄海水产研究所海水养殖疾病控制与分子病理学实验室;
- 论文关键词：蜡样芽孢杆菌 ; 生物膜 ; 凡纳滨对虾 ; 白斑综合征病毒 ; 副溶血弧菌
- 英文论文关键词：Bacillus cereus ; Biofilm ; Litopenaeus vannamei ; WSSV ; Vibrio parahaemolyticus ; intestinal microflora
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S968.22;S945.49
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：560k
- 原文格式：D
- 会议级别：全国
摘要
以基础饲料为空白组,在基础饲料中添加蜡样芽孢杆菌游离态活菌为游离态组,在基础饲料中添加蜡样芽孢杆菌活菌生物膜作为生物膜组,通过养殖和攻毒实验评价了外源补充蜡样芽孢杆菌生物膜对凡纳滨对虾生长、抗病力及对其肠道微生物组成的影响。结果显示:生物膜组和游离态组对虾体重、体长增长速率高于空白组,生物膜组与游离态组对虾体重、体长差异性不显著;生物膜组弧菌属含量平均为34.65%,游离态组弧菌属含量平均为39.27%,空白对照组弧菌属含量平均为58.00%;在WSSV攻毒实验中,生物膜组平均累积死亡率为80%,游离态组平均累积死亡率为77%;空白对照组平均累积死亡率为92%;在副溶血弧菌攻毒实验中,生物膜组平均累积死亡率为61.3%,游离态组平均累积死亡率为75%;空白对照组平均累积死亡率为77.3%。研究结果证实:饲料中添加蜡样芽孢杆菌生物膜和游离态蜡样芽孢杆菌投喂凡纳滨对虾后可改变对虾肠道的微生物组成,提高对虾生长速度,增强凡纳滨对虾的抗病能力,而且添加蜡样芽孢杆菌生物膜效果最明显。
In order to research the effect of Bacillus cereus biofilm on the growth rate, disease resistance and intestinal microflora of Litopenaeus vannamei, planktonic Bacillus cereus and Bacillus cereus biofilm was added to basic feed separately. Shrimps were challenged with white spot syndrome virus(WSSV) and Vibrio parahaemolyticus after 15 days of feeding experiment.The whole experiment was end after 40 days. The result showed the growth rate of the biofilm group and the planktonic group were higher than the control group. The proportion of Vibrio in the biofilm group 、 planktonic group and control group were 34.65% 、 39.27% and 58.00%. The cumulative mortality of the biofilm group, planktonic group and control group were 80%, 77%and 92% separately, after challenged by WSSV. The cumulative mortality of the biofilm group、planktonic group and control group were 61.3%, 75% and 77.3% separately, after challenged by Vibrio parahaemolyticus. This study suggest that dietary with Bacillus cereus biofilm and planktonic Bacillus cereus can change the intestinal microflora of Litopenaeus vannamei, improve the disease resistance and enhance the growth.
In order to research the effect of Bacillus cereus biofilm on the growth rate, disease resistance and intestinal microflora of Litopenaeus vannamei, planktonic Bacillus cereus and Bacillus cereus biofilm was added to basic feed separately. Shrimps were challenged with white spot syndrome virus(WSSV) and Vibrio parahaemolyticus after 15 days of feeding experiment.The whole experiment was end after 40 days. The result showed the growth rate of the biofilm group and the planktonic group were higher than the control group. The proportion of Vibrio in the biofilm group 、 planktonic group and control group were 34.65% 、 39.27% and 58.00%. The cumulative mortality of the biofilm group, planktonic group and control group were 80%, 77%and 92% separately, after challenged by WSSV. The cumulative mortality of the biofilm group、planktonic group and control group were 61.3%, 75% and 77.3% separately, after challenged by Vibrio parahaemolyticus. This study suggest that dietary with Bacillus cereus biofilm and planktonic Bacillus cereus can change the intestinal microflora of Litopenaeus vannamei, improve the disease resistance and enhance the growth.
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