健康与腹泻幼犬肠道菌群数量差异分析研究
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- 英文论文题名：The Comparison Test on the Intestinal Microflora of the Healthy and Diarrhea Puppies
- 论文作者：温建新 ; 王寿山 ; 单虎
- 英文论文作者：Wen Jianxin ; Wang shou-shan ; Shan-hu ; College of Animal Sciences ; Qindao Agriculture Univisty
- 年：2016
- 作者机构：青岛农业大学动物科技学院;
- 论文关键词：幼犬 ; 肠道菌群 ; 腹泻 ; 选择培养基
- 英文论文关键词：Puppies ; Intestinal flora ; Diarrhea ; Selective medium
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S858.292
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：66k
- 原文格式：O
- 会议级别：全国
摘要
[目的]通过对健康幼犬和腹泻幼犬的肠道菌群进行研究,对两者之间的差异进行探讨,以指导临床对腹泻的治疗。[方法]无菌采取健康幼犬和腹泻幼犬的粪便,通过选用不同选择性培养基,对其粪便微生物进行分离培养,比较两者菌群的差异。并筛选优势菌株,研究其生长特性以及通过生化试验进行确认。[结果]健康幼犬与腹泻幼犬粪便中常见细菌有肠杆菌、肠球菌、乳杆菌、双歧杆菌等,其数量的变化比较,腹泻幼犬粪便中肠球菌和乳酸杆菌的数量降低明显,双歧杆菌数量略有下降,肠杆菌的数量有不显著增加的趋势。[结论]肠道菌群失调易引起腹泻,临床上应正确治疗腹泻,避免滥用抗生素。
[Objective]Through the study on the litestinal flora of the healthy and diarrhea puppies,differences were explored between the two flora to guide the clinical treatment of diarrhea.[Method]After healthy and diarrhea puppy faeces were adopted aseptically,the faecal micro-organisms were isolated and cultured to compare the differences between the two flora through the different selective media.The growing characteristics and the appearance of bacterium were analyzed and identified.[Result]The intestinal flora includes Enterobacteria,Enterococcus,Lactobacillus,Bifidobacterium etc.Compared with the number of bacteria in the intestinal canal of the healthy puppy.in the the intestinal canal of the diarrhea puppy,the number of Enterococci and Lactobacilli declined obviously,the number of Bifidobacteria declined little and the number of Enterobacteria had no significant increasing trend.[Conclusion]Imbalance of intestinal flora was caused easily by the diarrhea,so the clinical treatment of diarrhea should be corrected to avoid the abuse of antibiotics.
[Objective]Through the study on the litestinal flora of the healthy and diarrhea puppies,differences were explored between the two flora to guide the clinical treatment of diarrhea.[Method]After healthy and diarrhea puppy faeces were adopted aseptically,the faecal micro-organisms were isolated and cultured to compare the differences between the two flora through the different selective media.The growing characteristics and the appearance of bacterium were analyzed and identified.[Result]The intestinal flora includes Enterobacteria,Enterococcus,Lactobacillus,Bifidobacterium etc.Compared with the number of bacteria in the intestinal canal of the healthy puppy.in the the intestinal canal of the diarrhea puppy,the number of Enterococci and Lactobacilli declined obviously,the number of Bifidobacteria declined little and the number of Enterobacteria had no significant increasing trend.[Conclusion]Imbalance of intestinal flora was caused easily by the diarrhea,so the clinical treatment of diarrhea should be corrected to avoid the abuse of antibiotics.
引文
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[2]闻玉梅,主编.现代医学微生物学[M].上海:上海医科大学出版社,1999.97-112.
[3]刘运德,楼永良.微生物学检验[M].第2版.北京:人民卫生出版社,2003.84-86.
[4]吴仲文,李兰娟,马伟杭等.肠道微生物定植抗力的新指标—值[J].浙江预防医学,2000,12:4-5.
[5]祝小枫,熊德鑫,李小燕等.几种动物的膜菌群与肠内容物菌群的比较[J].中国实验动物学杂志.2001.5:30-32.
[6]Hansen AK,Velschow S.Antibiotic resistance in bacterial isolates from laboratory animal colonies naive to antibiotic treatment[J].Lab Anim,2000.34:413-422.
[7]盛茂地,黄金娥,孵9秀平等..建立一种粪便细菌学检验的新方法-肠道菌谱分析[J].中国感染控制杂志,2005,4(2):120-123.