富集技术用于近红外光谱定量分析复杂样品中的微量物质
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- 英文论文题名：Determination of Micro Substances in Complex Samples by Near-Infrared Spectroscopy with Preconcentration
- 论文作者：王翠翠 ; 杨亚非 ; 蔡文生 ; 邵学广
- 英文论文作者：Cuicui Wang ; Yafei Yang ; Wensheng Cai ; Xueguang Shao ; Research Center for Analytical Sciences ; Department of Chemistry ; Nankai University
- 年：2016
- 作者机构：南开大学化学学院分析科学研究中心;
- 论文关键词：近红外光谱 ; 富集 ; 多元校正 ; 定量
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十五分会：化学信息学与化学计量学
- 语种：中文
- 分类号：O657.33
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十五分会 ; 化学信息学与化学计量学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：142k
- 原文格式：D
- 会议级别：全国
摘要
近红外光谱以其丰富的信息含量及和化学计量学的结合,被广泛应用在复杂样品的定性、定量分析中。然而,近红外漫反射光谱分析方法存在检测限较高,灵敏度较低等缺点,使其多局限于对常量物质的分析检测。为降低其检测限,本课题组提出了富集技术与近红外漫反射光谱分析相结合的方法。首先通过制备的富集材料高效富集复杂基质中的目标组分,然后采集近红外漫反射光谱并结合多元校正技术进行定量计算。已将该方法成功用于复杂基质中微量组分的定量检测,如河水中的金属离子~([1-4]),果汁中的有机酸~([5]),工业污水中的酚类物质~([6]),稀溶液中的脱氧核糖核酸~([7])和微体积样品中的牛血清蛋白~([8])等,实现ppm-level的检测,大大拓宽了近红外光谱技术的应用范围。
Near infrared spectroscopy,with abundant information and in combination with chemometrics,was widely used in the qualitative and quantitative analysis of complex samples.However,the high detection limit or low sensitivity of near infrared diffuse reflectance spectroscopy(NIRDRS) makes it more confined to the macro-component determination.To improve the sensitivity,preconcentration was combined with NIRDRS.The target component in complex matrix was enriched by prepared material firstly,and then NIR spectra of samples were measured.At last,multivariate calibration was used for quantitative calculation.In our works,the method has been used for determination of low content substances,such as metal ions in river water~([1-4]),organic acids in orange juice~([5]),phenols in wastewater~([6]),deoxyribonucleic acid in dilute solution~([7]),BSA in micro-volume samples~([8]),etc.These works achieved the ppm-level detection,and greatly broadened the application of near-infrared spectroscopy.
Near infrared spectroscopy,with abundant information and in combination with chemometrics,was widely used in the qualitative and quantitative analysis of complex samples.However,the high detection limit or low sensitivity of near infrared diffuse reflectance spectroscopy(NIRDRS) makes it more confined to the macro-component determination.To improve the sensitivity,preconcentration was combined with NIRDRS.The target component in complex matrix was enriched by prepared material firstly,and then NIR spectra of samples were measured.At last,multivariate calibration was used for quantitative calculation.In our works,the method has been used for determination of low content substances,such as metal ions in river water~([1-4]),organic acids in orange juice~([5]),phenols in wastewater~([6]),deoxyribonucleic acid in dilute solution~([7]),BSA in micro-volume samples~([8]),etc.These works achieved the ppm-level detection,and greatly broadened the application of near-infrared spectroscopy.
引文
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[8]Cui,C.J.;Cai,W.S.;Shao,X.G.Chinese Chem.Lett.2013,24:67.
[2]Sheng,N.;Cai,W.S.;Shao,X.G.Talanta 2009,79:339.
[3]Ning,Y.;Li,J.H.;Cai,W.S.;Shao,X.G.Spectrochim.Acta A 2012,96:289.
[4]Liu,Y.;Ning,Y.;Cai,W.S.;Shao,X.G.Analyst 2013,138:6617.
[5]Hao,Y.;Cai,W.S.;Shao,X.G.Spectrochim.Acta A 2009,72:115.
[6]Zhang,Y.;Hao,Y.;Cai,W.S.;Shao,X.G.Anal.Method 2011,3:703.
[7]Yang,Y.F.;Tu,J.R.;Cai,W.S.;Shao,X.G.Talanta 2012,99:871.
[8]Cui,C.J.;Cai,W.S.;Shao,X.G.Chinese Chem.Lett.2013,24:67.