赤星病胁迫对不同抗性烟草品种光合作用和叶绿素荧光特性的影响
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- 英文论文题名：Impacts of Alternaria alternata stress on characteristics of photosynthesis and chlorophyll fluorescence in two tobacco cultivars with different resistances
- 论文作者：杨志晓 ; 丁燕芳 ; 张小全 ; 薛刚 ; 王轶 ; 任学良 ; 任周营 ; 杨铁钊
- 英文论文作者：YANG Zhixiao ; DING Yangfang ; ZHANG Xiaoquan ; XUE Gang ; WANG Yi ; REN Xueling ; REN Zhouying ; YANG Tiezhao ; Guizhou Academy of Tobacco Science ; College of Tobacco Science ; Henan Agricultural University ; Tobacco Research Institute of Henan Academy of Agricultural Sciences ; China Tobacco Jiangxi Provincial Industrial Company
- 年：2016
- 作者机构：贵州省烟草科学研究院育种工程技术中心;河南农业大学烟草学院;河南省农业科学院烟草研究所;江西中烟工业公司;
- 论文关键词：烟草 ; 烟草赤星病 ; 光合色素 ; 光合特性 ; 叶绿素荧光参数
- 英文论文关键词：tobacco ; tobacco brown spot ; photosynthetic pigment ; photosynthetic characteristics ; chlorophyll fluorescence parameters
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：17
- 文件大小：1398k
- 原文格式：D
摘要
【目的】为研究烟草赤星病胁迫对光合作用作用和叶绿素荧光特性的影响。【方法】以不同烟草赤星病抗性品种JYH(抗病品种)和CBH(感病品种)为材料,采用盆栽试验,调查不同烟草赤星病胁迫程度(轻度胁迫、中度胁迫和重度胁迫)对光合色素含量、光合作用参数和叶绿素荧光动力学特征的影响。【结果】1.烟草赤星病胁迫导致2个品种的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均呈下降趋势,JYH的降幅较小。2.除JYH的净光合速率在轻度胁迫下有所增加外,JYH、CBH的净光合速率、气孔导度因赤星病胁迫而降低。烟草赤星病胁迫导致CBH的胞间CO_2浓度上升,气孔限制值则明显下降,这与JYH在重度胁迫下的变化趋势一致。而JYH的胞间CO_2浓度在轻度、中度胁迫降低,气孔限制值则呈上升趋势。3.2个品种的初始荧光(F_0)、非光化学猝灭系数(NPQ)在赤星病胁迫下均有所增加;而各品种的最大荧光(F_m)、可变荧光(F_v)、PSII最大光化学效率(F_v/F_m)、PSII潜在活性(F_v/F_0)、光化学淬灭系数(q_p)、PSII实际光化学效率(Φ_(PSII))在赤星病胁迫下均呈下降趋势,CBH的降幅较大。【结论】JYH的光合色素、光合作用和叶绿素荧光特性受烟草赤星病胁迫影响小于CBH,维持较高的光合性能是对赤星病具有较强抗性的生理基础。
The responses of photosynthetic pigment content, photosynthetic parameters and chlorophyll fluorescence kinetic characteristics were investigated under three different stress levels of tobacco brown spot(mild, moderate and severe stress) at maturity using pot cultures. Two tobacco cultivars with different resistances to tobacco brown spot were selected as experimental materials to determine whether there was a correlation between photosynthetic characteristics and resistance. The cultivars selected were JYH(resistant cultivar) and CBH(sensitive cultivar). The results showed that brown spot stress could decrease chlorophyll a, chlorophyll b, total chlorophyll and carotenoids in the two cultivars. In addition, the decreased range of photosynthetic pigments was less in JYH than in CBH. Tobacco brown spot stress decreased both the net photosynthetic rate and stomatal conductance. However, the net photosynthetic rate of JYH increased under mild stress whereas there was a decrease under moderate and severe stress. In the two cultivars, the changes in intercellular CO_2 concentration and stomatal conductance were divergent. The intercellular CO_2 concentration in CBH tended to rise under the three different stress levels, whereas the stomatal limitation value showed a significant decrease. In JYH under severe stress, the photosynthetic parameters consisting of both intercellular CO_2 concentration and stomatal conductance showed the same trend as in CBH. However, compared with the rising trend of intercellular CO_2 concentration under severe stress, the stomatal limitation value of JYH decreased under both mild and moderate stress. With the increasing stress levels, the initial fluorescence(F_0) and non-photochemical quenching(NPQ) of the two cultivars both increased. However, the chlorophyll fluorescence kinetic parameters including maximal fluorescence(F_m), variable fluorescence(F_v), maximal photochemical efficiency(F_v/F_m), PSII potential efficiency(F_v/F_0), photochemical quenching coefficient(q_p) and PSII actual quantum yield(Φ_(PSII)) all decreased by varying degrees under the three stress levels, and the decreased range was less in JYH than in CBH. These results indicate that tobacco brown spot had a destructive effect on photosynthetic characteristics. The photosynthetic pigments, photosynthesis and chlorophyll fluorescence characteristics of JYH were less influenced by brown spot stress than CBH. So a higher photosynthetic performance may be a major physiological trait of resistant cultivars in the adaptability to tobacco brown spot infection.
The responses of photosynthetic pigment content, photosynthetic parameters and chlorophyll fluorescence kinetic characteristics were investigated under three different stress levels of tobacco brown spot(mild, moderate and severe stress) at maturity using pot cultures. Two tobacco cultivars with different resistances to tobacco brown spot were selected as experimental materials to determine whether there was a correlation between photosynthetic characteristics and resistance. The cultivars selected were JYH(resistant cultivar) and CBH(sensitive cultivar). The results showed that brown spot stress could decrease chlorophyll a, chlorophyll b, total chlorophyll and carotenoids in the two cultivars. In addition, the decreased range of photosynthetic pigments was less in JYH than in CBH. Tobacco brown spot stress decreased both the net photosynthetic rate and stomatal conductance. However, the net photosynthetic rate of JYH increased under mild stress whereas there was a decrease under moderate and severe stress. In the two cultivars, the changes in intercellular CO_2 concentration and stomatal conductance were divergent. The intercellular CO_2 concentration in CBH tended to rise under the three different stress levels, whereas the stomatal limitation value showed a significant decrease. In JYH under severe stress, the photosynthetic parameters consisting of both intercellular CO_2 concentration and stomatal conductance showed the same trend as in CBH. However, compared with the rising trend of intercellular CO_2 concentration under severe stress, the stomatal limitation value of JYH decreased under both mild and moderate stress. With the increasing stress levels, the initial fluorescence(F_0) and non-photochemical quenching(NPQ) of the two cultivars both increased. However, the chlorophyll fluorescence kinetic parameters including maximal fluorescence(F_m), variable fluorescence(F_v), maximal photochemical efficiency(F_v/F_m), PSII potential efficiency(F_v/F_0), photochemical quenching coefficient(q_p) and PSII actual quantum yield(Φ_(PSII)) all decreased by varying degrees under the three stress levels, and the decreased range was less in JYH than in CBH. These results indicate that tobacco brown spot had a destructive effect on photosynthetic characteristics. The photosynthetic pigments, photosynthesis and chlorophyll fluorescence characteristics of JYH were less influenced by brown spot stress than CBH. So a higher photosynthetic performance may be a major physiological trait of resistant cultivars in the adaptability to tobacco brown spot infection.
引文
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[8]郭兴启,李向东,朱汉城,等.马铃薯Y病毒(PVY)的侵染对烟草叶片光合作用的影响[J].植物病理学报,2000,30(1):94-95.GUO Xingqi,LI Xiangdong,ZHU Hancheng,et al.Effect of PVY-infection on photosynthesis of tobacco[J].Acta Phytopathologica Sinica,2000,30(1):94-95.
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