两种微生物菌株组合处理对玉米秸秆养分及有氧稳定性的影响
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摘要
本试验旨在研究产几丁质酶枯草芽孢杆菌BS-1及产酸植物乳杆菌LABP组合处理对抑制玉米秸秆霉变的效果。试验玉米秸秆含水量为20%,添加BS-1+LABP处理,以0.5%丙酸为阳性对照,无处理为空白对照。密闭真空保存30d后开封进行有氧存放,于存放期内取样进行有氧稳定性、养分含量测定,并对玉米秸秆中的霉菌数量的变化进行检测。结果表明:玉米秸秆霉变初期DM、淀粉含量显著下降,而CP、NDF含量呈上升趋势。随着霉变程度加重,各常规营养指标变化幅度增加,以淀粉相对损失率最高。当玉米秸秆含水量为20%时,添加107cfu/g的BS-1+LABP复合菌液处理能够在一定程度上抑制玉米秸秆中霉菌的生长,增加玉米秸秆的有氧稳定性,降低其营养成分损失率及霉菌总数。
引文
[1]史海涛,杨军香,李胜利等.玉米秸秆营养价值的开发利用—未充分开发利用的廉价资源[J].中国奶牛,2012(17):3-11.
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[3]Kishore G K,Pande S,Podile A R.Biological control of late leaf spot of peanut(Arachis hypoga ea)with chitinolytic bacteria[J].Phytopathology,2005,95(10):1157-1165.
[4]Mehmood M A,Xiao X,Hafeez F Y,et al.Purification and characterization of a chitinase from S erratia proteamaculans[J].World Journal of Microbiology and Biotechnology,2009,25(11):1955-1961.
[5]PATIL N S,WAGHMARE S R,JADHAV J P.Purification and characterization of an extracellular ant ifungal chitinase from Penicillium ochrochloron MTCC 517 and its application in protoplast formation[J].Process Biochemistry,2012,48(1):176–183.
[6]赵秋敏,陈月华,蔡峻,等.一株产几丁质酶、抑真菌的侧孢芽孢杆菌.中国生物防治,2006,22:42-46.
[7]Roberts W K,Selitrennikoff C P.Plant and bacterial chitinases differ in antifungal activity[J].Jour nal of General Microbiology,1988,(1):169-176.
[8]全国饲料工业标准化技术委员会.GB/T6435-2014饲料中水分的测定[S].北京:中国标准出版社2014.
[9]杨胜.饲料分析与饲料质量检测技术[M].北京:中国农业大学出版社,1994.
[10]全国饲料工业标准化技术委员会.GB/T13092-2006饲料中霉菌总数的测定[S].北京:中国标准出版社,2006.
[11]魏金涛,张妮娅,齐德生,等.四种常用饲料原料生霉后品质变化规律研究[J].中国粮油学报,2007,22(5):119-124.
[12]Dixon R C,Hamilton P B.Effect of Feed Ingredients on the Antifungal Activity of Propionic A cid[J].Poultry Science,1981,60(11):2407-2411.
[13]张伟毅,史莹华,姚惠霞,等.不同温度下苜蓿草捆霉变规律及草捆品质变化[J].草业科学,2010(11):145-150.
[14]李改英.淋雨对苜蓿霉变的影响与苜蓿优化青贮技术的研究[D].河南农业大学,2009.
[15]李洋,辛杭书,李春雷,等.奶牛常用秸秆类饲料营养价值的评定[J].东北农业大学学报,2015(4):76-82.
[16]荣磊.苜蓿草捆贮藏品质调控方法研究[D].内蒙古农业大学,2013.
[17]计成.动物营养学[M].北京:高等教育出版社,2008:9-13.
[18]魏金涛,齐德生.饲料霉变品质变化及其评价指标探讨[J].饲料工业,2006(11):49-51.
[19]Bryden W L.Mycotoxin contamination of the feed supply chain:Implications for animal productivity and feed security[J].Animal Feed Science and Technology,2012,173(1-2):134-158.
[20]Maciorowski K G,Herrera P,Jones F T,et al.Effects on poultry and livestock of feed contamin ation with bacteria and fungi[J].Animal Feed Science and Technology,2007,133(1–2):109-136.
[2]中华人民共和国农业部.全国农作物秸秆资源调查与评价报告[R].2010.
[3]Kishore G K,Pande S,Podile A R.Biological control of late leaf spot of peanut(Arachis hypoga ea)with chitinolytic bacteria[J].Phytopathology,2005,95(10):1157-1165.
[4]Mehmood M A,Xiao X,Hafeez F Y,et al.Purification and characterization of a chitinase from S erratia proteamaculans[J].World Journal of Microbiology and Biotechnology,2009,25(11):1955-1961.
[5]PATIL N S,WAGHMARE S R,JADHAV J P.Purification and characterization of an extracellular ant ifungal chitinase from Penicillium ochrochloron MTCC 517 and its application in protoplast formation[J].Process Biochemistry,2012,48(1):176–183.
[6]赵秋敏,陈月华,蔡峻,等.一株产几丁质酶、抑真菌的侧孢芽孢杆菌.中国生物防治,2006,22:42-46.
[7]Roberts W K,Selitrennikoff C P.Plant and bacterial chitinases differ in antifungal activity[J].Jour nal of General Microbiology,1988,(1):169-176.
[8]全国饲料工业标准化技术委员会.GB/T6435-2014饲料中水分的测定[S].北京:中国标准出版社2014.
[9]杨胜.饲料分析与饲料质量检测技术[M].北京:中国农业大学出版社,1994.
[10]全国饲料工业标准化技术委员会.GB/T13092-2006饲料中霉菌总数的测定[S].北京:中国标准出版社,2006.
[11]魏金涛,张妮娅,齐德生,等.四种常用饲料原料生霉后品质变化规律研究[J].中国粮油学报,2007,22(5):119-124.
[12]Dixon R C,Hamilton P B.Effect of Feed Ingredients on the Antifungal Activity of Propionic A cid[J].Poultry Science,1981,60(11):2407-2411.
[13]张伟毅,史莹华,姚惠霞,等.不同温度下苜蓿草捆霉变规律及草捆品质变化[J].草业科学,2010(11):145-150.
[14]李改英.淋雨对苜蓿霉变的影响与苜蓿优化青贮技术的研究[D].河南农业大学,2009.
[15]李洋,辛杭书,李春雷,等.奶牛常用秸秆类饲料营养价值的评定[J].东北农业大学学报,2015(4):76-82.
[16]荣磊.苜蓿草捆贮藏品质调控方法研究[D].内蒙古农业大学,2013.
[17]计成.动物营养学[M].北京:高等教育出版社,2008:9-13.
[18]魏金涛,齐德生.饲料霉变品质变化及其评价指标探讨[J].饲料工业,2006(11):49-51.
[19]Bryden W L.Mycotoxin contamination of the feed supply chain:Implications for animal productivity and feed security[J].Animal Feed Science and Technology,2012,173(1-2):134-158.
[20]Maciorowski K G,Herrera P,Jones F T,et al.Effects on poultry and livestock of feed contamin ation with bacteria and fungi[J].Animal Feed Science and Technology,2007,133(1–2):109-136.