造纸法再造烟叶涂布液中腐败菌的分离鉴定与抑菌
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- 英文论文题名：Isolation, Identification and Bacteriostasis of Spoilage Bacteria in Coating solution of Paper-Making Reconstituted Tobacco Sheet
- 论文作者：田海龙 ; 孙伟 ; 高颖 ; 李玲 ; 李英 ; 陈丽君 ; 陈茂深 ; 钟芳
- 英文论文作者：TIAN Hailong ; SUN Wei ; GAO Ying ; LI Ling ; LI Ying ; CHEN Lijun ; CHEN Maoshen ; ZHONG Fang ; Shandong Rebirth Tobacco Co. ; LTD ; School of Food Science and Technology ; Jiangnan University
- 年：2016
- 作者机构：山东瑞博斯烟草有限公司;江南大学食品学院;
- 论文关键词：涂布液 ; 细菌 ; 分离 ; 鉴定 ; 抑菌
- 英文论文关键词：Coating soultion ; Bacteria ; Isolation ; Identification ; Bacteriostasis
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：中文
- 分类号：TS41
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：6
- 文件大小：1302k
- 原文格式：D
摘要
本文采用平板划线分离方法对造纸法再造烟叶涂布液中的主要微生物群进行分离,再根据细菌的菌落形态、菌体形态、生理生化实验和16S r DNA序列分析对菌株进行了鉴定。结果表明,鉴定出引起涂布液腐败的细菌为短小芽孢杆菌,地衣芽孢杆菌和肺炎克雷伯菌,其中肺炎克雷伯菌为主要腐败菌。此外,本文还考察了山梨酸钾、双乙酸钠、尼泊金酯钠、乙二胺四乙酸二钠和ε-聚赖氨酸等五种防腐剂对肺炎克雷伯菌的抑菌效果。结果表明5种防腐剂的抑菌效果强弱顺序为ε-聚赖氨酸>乙二胺四乙酸二钠>双乙酸钠>山梨酸钾>尼泊金酯钠,其中ε-聚赖氨酸对肺炎克雷伯菌的最小抑菌浓度为0.075 mg/m L。
In this paper, the streak plate separation method was used to classify and identify the primary bacterial flora in the coating solution of paper-making reconstituted tobacco sheet according to colony shape, bacterial characteristics, physio-biochemical tests as well as 16 S r DNA sequence analysis. The results indicated that the spoilage bacteria in the coating solution of paper-making reconstituted tobacco sheet were Bacillus pumilus, Bacillus licheniformis and Klebsiella pneumoniae, Klebsiella pneumoniae was the main spoilage bacteria. Besides, the antibacterial effects of five food preservatives(potassium sorbate, sodium diacetate, butylparaben sodium, EDTA-2Na and ε-polylysine(ε-PL)) on the spoilage bacteria were evaluated. The results revealed that these five preservatives could be ranked in decreasing order of antibacterial activity as follows: ε-PL> EDTA-2Na > sodium diacetate > potassium sorbate > butylparaben sodium. The minimal inhibitory concentrations of ε-PL to Klebsiella pneumoniae was 0.075 mg/m L.
In this paper, the streak plate separation method was used to classify and identify the primary bacterial flora in the coating solution of paper-making reconstituted tobacco sheet according to colony shape, bacterial characteristics, physio-biochemical tests as well as 16 S r DNA sequence analysis. The results indicated that the spoilage bacteria in the coating solution of paper-making reconstituted tobacco sheet were Bacillus pumilus, Bacillus licheniformis and Klebsiella pneumoniae, Klebsiella pneumoniae was the main spoilage bacteria. Besides, the antibacterial effects of five food preservatives(potassium sorbate, sodium diacetate, butylparaben sodium, EDTA-2Na and ε-polylysine(ε-PL)) on the spoilage bacteria were evaluated. The results revealed that these five preservatives could be ranked in decreasing order of antibacterial activity as follows: ε-PL> EDTA-2Na > sodium diacetate > potassium sorbate > butylparaben sodium. The minimal inhibitory concentrations of ε-PL to Klebsiella pneumoniae was 0.075 mg/m L.
引文
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[9]杨晓韬,李春,周晓宏.7种食品防腐剂对肉制品污染微生物的抑菌效果比较研究[J].食品科学,2012,33(11):12-16.
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[13]杨俊,刘江生,蔡继宝,等.高效液相色谱-蒸发光散射检测法测定烟草中的水溶性糖[J].分析化学,2006,33(11):1596-1598.
[14]周元清,吴兆录,史云东,等.高效液相色谱法测定烟草料液中的糖[J].分析试验室,2006,25(9):71-74.
[2]唐兴平,陈学榕,戴达松,等.烟草废弃物造纸法制烟草薄片[J].福建农林大学学报:自然科学版,2007,36(2):205-207.
[3]韩文佳,赵传山.造纸法烟草薄片发展现状[J].黑龙江造纸,2008,35(4):47-49.
[4]许日鹏,苏文强,段继生.烟草薄片的开发与应用[J].上海造纸,2009,39(6):46-49.
[5]李东亮.基于化学成分的烟草质量评价方法研究与应用[D].郑州:河南农业大学,2008.
[6]郎涛,孙香彬,侯君,等.食品微生物检验中菌落总数测定的注意事项[J].微生物学杂志,2007,27(3):111-112.
[7]朱维军,焦镭,石明生,等.冷却肉中常见腐败细菌的分离与鉴定[J].中国农学通报,2009,25(18):99-101.
[8]王英,周剑忠,黄开红,等.泡菜中一株植物乳杆菌的分离筛选及鉴定[J].江苏农业学报,2010,26(1):219-221.
[9]杨晓韬,李春,周晓宏.7种食品防腐剂对肉制品污染微生物的抑菌效果比较研究[J].食品科学,2012,33(11):12-16.
[10]王敏,檀建新,路玲,等.非发酵豆制品主要腐败菌的分离鉴定[J].中国酿造,2006,25(6):68-70.
[11]Clayton R A,Sutton G,HINKLE P S,et al.Intraspecific variation in small-subunit r RNA sequences in Gen Bank:why single sequences may not adequately represent prokaryotic taxa[J].International journal of systematic bacteriology,1995,45(3):595-599.
[12]Kolbert C P,Persing D H.Ribosomal DNA sequencing as a tool for identification of bacterial pathogens[J].Current opinion in microbiology,1999,2(3):299-305.
[13]杨俊,刘江生,蔡继宝,等.高效液相色谱-蒸发光散射检测法测定烟草中的水溶性糖[J].分析化学,2006,33(11):1596-1598.
[14]周元清,吴兆录,史云东,等.高效液相色谱法测定烟草料液中的糖[J].分析试验室,2006,25(9):71-74.