猪圆环病毒海南株全基因组分析
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- 英文论文题名：Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus isolated from Hainan Province
- 论文作者：曹宗喜 ; 贺冬梅 ; 郑心力 ; 张艳 ; 叶保国 ; 林哲敏 ; 王峰
- 英文论文作者：CAO Zong-xi ; HE Dongmei ; ZHENG Xin-li ; ZHANG Yan ; YE Bao-guo ; LIN Zhe-min ; WANG Feng ; Institute of Animal Husbandry and Veterinary Medicine ; Hainan Academy of Agricultural Sciences
- 年：2016
- 作者机构：海南省农业科学院畜牧兽医研究所;
- 论文关键词：猪圆环病毒2型 ; 全基因组 ; 克隆 ; 序列分析
- 英文论文关键词：Porcine circovirus type 2 ; complet genome ; Cloning ; Sequence Analysis
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：中文
- 分类号：S852.65
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：9
- 文件大小：626k
- 原文格式：D
- 会议级别：全国
摘要
为了研究猪圆环病毒2型海南株的分子特征,采用PCR方法从疑似PCV2感染的猪组织病料中扩增基因并进行序列分析。结果表明:8株PCV2海南株均属于PCV2b型,其中7株为PCV2b-1C亚型,1株为PCV2b-1A/1B亚型。PCV2海南株的ORF2基因的长度均为705 bp,编码234个氨基酸。Cap蛋白的抗原表位区发生了一定的变异。8个PCV2海南株的ORF2基因之间核苷酸序列相似性及推导的氨基酸序列相似性分别为95.3%~99.7%和93.6%~100%。海南株与国内分离株(AY682994,AF381175,JX945577,JX682407,AY180397)的核苷酸序列相似性为91.0%~99.9%,推导的氨基酸相似性为91.0%~99.6%。海南株与国外分离株(NC_005148,JQ994268,KJ187306,AF201307,AF454546,AY754020)的核苷酸序列相似性为90.0%~97.0%,推导的氨基酸相似性为88.0%~97.9%。海南株与疫苗株SH的核苷酸序列相似性为98.2%~100.0%,推导的氨基酸相似性为94.9%~100.0%。海南株与疫苗株LG的核苷酸序列相似性为90.6%~91.7%,推导的氨基酸相似性为89.7%~91.0%。这些数据为海南地区PCV2的防控和疫苗株选择提供了理论依据。
To understand molecular characteristics of PCV2 isolated from Hainan Province,some PMWS-suspected samples from Hainan province were amplified the complete genome of PCV2 and made sequence analysis.The results showed that a total of 8 PCV2 strains was isolated and identified,and all the isolates belonged to genotype 2b,of which 7 isolates were grouped into cluster PCV2b-1C and 1 isolates were grouped into clusters PCV2b-1A/1B.The ORF2 gene of 8 PCV2 strains were 705 bp,encoding 234 amino acids.The main antigen epitope area has changed significantly in Cap protein of PCV2.The identity of PCV2 ORF2 sequence among 8 isolates was 95.3%~99.7% of nucleotide similarity,and 93.6%~100% of deduced amino acid similarity.There has 91.0%-99.9% nucleotide similarity and 91.0%-99.6% deduced amino acid similarity between AY682994,AF381175,JX945577,JX682407 and AY180397;90.0%-97.0% nucleotide similarity and88.0%-97.9% deduced amino acid similarity between NC_005148,JQ994268,KJ187306,AF201307,AF454546 and AY754020;98.2%-100.0% nucleotide similarity and 94.9%-100.0% deduced amino acid similarity between SH;90.6%-91.7% nucleotide similarity and 89.7%-91.0% deduced amino acid similarity between LG.This study got the basic epidemiological data for the prevention and control of PCV-2 in Hainan Province,it can also help the choice of PCV-2 vaccine.
To understand molecular characteristics of PCV2 isolated from Hainan Province,some PMWS-suspected samples from Hainan province were amplified the complete genome of PCV2 and made sequence analysis.The results showed that a total of 8 PCV2 strains was isolated and identified,and all the isolates belonged to genotype 2b,of which 7 isolates were grouped into cluster PCV2b-1C and 1 isolates were grouped into clusters PCV2b-1A/1B.The ORF2 gene of 8 PCV2 strains were 705 bp,encoding 234 amino acids.The main antigen epitope area has changed significantly in Cap protein of PCV2.The identity of PCV2 ORF2 sequence among 8 isolates was 95.3%~99.7% of nucleotide similarity,and 93.6%~100% of deduced amino acid similarity.There has 91.0%-99.9% nucleotide similarity and 91.0%-99.6% deduced amino acid similarity between AY682994,AF381175,JX945577,JX682407 and AY180397;90.0%-97.0% nucleotide similarity and88.0%-97.9% deduced amino acid similarity between NC_005148,JQ994268,KJ187306,AF201307,AF454546 and AY754020;98.2%-100.0% nucleotide similarity and 94.9%-100.0% deduced amino acid similarity between SH;90.6%-91.7% nucleotide similarity and 89.7%-91.0% deduced amino acid similarity between LG.This study got the basic epidemiological data for the prevention and control of PCV-2 in Hainan Province,it can also help the choice of PCV-2 vaccine.
引文
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[17]Harding J C,Baker C D,Tumber A,et al.Porcine circovirus-2 DNA concentration distinguishes wasting from nonwasting pigs and is correlated with lesion distribution,severity,and nucleocapsid staining intensity[J].J Vet Diagn Invest.2008,20(3):274-282.
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[21]程伟,张志,吴发兴,等.7株猪圆环病毒2型ORF2基因的克隆及序列分析[J].西北农业学报,2011,20(5):25-30.
[22]Stevenson L S,Gilpin D F,Douglas A,et al.T lymphocyte epitope mapping of porcine circovirus type 2[J].Viral Immunology,2007,20(3):389-397.
[2]Rose N,Opriessnig T,Grasland B,et al.Epidemiology and transmission of porcine circovirus type 2(PCV2)[J].Virus Res,2012,164(1-2):78-89.
[3]Opriessnig T,Meng X J,Halbur P G.Porcine circovirus type 2 associated disease:update on current terminology,clinical manifestations,pathogenesis,diagnosis,and intervention strategies[J].J Vet Diagn Invest,2007,19(6):591-615.
[4]Olvera A,Cortey M,Segales J.Molecular evolution of porcine circovirus type 2 genomes:phylogeny and clonality[J].Virology,2007,357(2):175-185.
[5]Cai L,Ni J,Xia Y,et al.Identification of an emerging recombinant cluster in porcine circovirus type 2[J].Virus Res,2012,165(1):95-102.
[6]An D J,Roh I S,Song D S,et al.Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006[J].Virus Res,2007,129(2):115-122.
[7]Segales J,Olvera A,Grau-Roma L,et al.PCV-2 genotype definition and nomenclature[J].Vet Rec,2008,162(26):867-868.
[8]束陈宇,许秋阳,段世鹏,等.猪圆环病毒2型江苏分离株的遗传进化分析[J].中国兽医学报,2014,34(7):1047-1052.
[9]徐廷川,张亮权,王衡,等.2007-2011年广东省猪圆环病毒2型分离株的遗传变异分析[J].中国兽医科学,2013,42(3):233-237.
[10]Huang Z,Su S,Wei C Y,et al.Complete genome sequence of a novel field strain of rearranged porcine circovirus type 2 in southern China[J].J Virol,2012,86(19):10895.
[11]李海超,韩凌霞.猪圆环病毒2型重组病毒分离株的鉴定[J].动物医学进展,2014,35(6):26-31.
[12]Cadar D,Cságola A,Lorincz M,et al.Detection of natural inter-and intra-genotype recombination events revealed by cap gene analysis and decreasing prevalence of PCV2 in wild boars[J].Infect Genet Evol,2012,12(2):420-427.
[13]Ramos N,Mirazo S,Castro G,et al.Molecular analysis of Porcine Circovirus Type 2 strains from Uruguay:evidence for natural occurring recombination[J].Infect Genet Evol,2013,19:23-31.
[14]何逸民,孔留五,罗玉均,等.2株猪圆环病毒2型分离毒株全基因组的克隆及序列分析[J].华南农业大学学报,2008,29(3):80-84.
[15]管萌,宿春虎,许晨旭,等.江苏部分地区猪圆环病毒2型的分子流行病学检测[J].中国动物检疫,2014,31(2):62-66.
[16]梁望旺,曾政,吴胜昔,等.重庆地区猪圆环病毒2型的分离鉴定与序列分析[J].动物医学进展,2013,34(12):55-59.
[17]Harding J C,Baker C D,Tumber A,et al.Porcine circovirus-2 DNA concentration distinguishes wasting from nonwasting pigs and is correlated with lesion distribution,severity,and nucleocapsid staining intensity[J].J Vet Diagn Invest.2008,20(3):274-282.
[18]Grenfell B T,Pybus O G,Gog J R,et al.Unifying the epidemiological and evolutionary dynamics of pathogens[J].Science,2004,303(5656):327-332.
[19]MahéD,Blanchard P,Truong C,et al.Differential recognition of ORF2 protein from type 1 and type 2porcine circoviruses and identification of immunorelevant epitopes[J].J Gen Virol,2000,81(7):1815-1824.
[20]Lekcharoensuk P1,Morozov I,Paul P S,et al.Epitope mapping of the major capsid protein of type 2porcine circovirus(PCV2)by using chimeric PCV1 and PCV2[J].J Virol,2004,78(15):8135-8145.
[21]程伟,张志,吴发兴,等.7株猪圆环病毒2型ORF2基因的克隆及序列分析[J].西北农业学报,2011,20(5):25-30.
[22]Stevenson L S,Gilpin D F,Douglas A,et al.T lymphocyte epitope mapping of porcine circovirus type 2[J].Viral Immunology,2007,20(3):389-397.