细胞的电泳与介电泳
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- 英文论文题名：Electrophoresis and dielectrophoresis of polarizable cells
- 论文作者：陈义
- 英文论文作者：Yi Chen ; Institute of Chemistry ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院化学研究所;
- 论文关键词：介电泳 ; 电泳 ; 细胞 ; 淌度测定 ; 微流控芯片
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：Q-33
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：134k
- 原文格式：D
- 会议级别：全国
摘要
电泳利用电场强迫带电粒子沿电场方向运动,而介电泳则通过诱导极化迫使颗粒在不均匀电场中运动,那么有办法利用某种电场迫使颗粒物同时进行电泳和介电泳吗?这显然是一个挑战性课题,不仅有趣,而且可能具有实际用途。我们因此进行的一些理论探索表明有实现的可能,因之利用微流控芯片技术进行了实证,结果证明,只要设计合理的芯片通道并施加合适的电场,就可以令可极化的带电粒子如细胞等物质同时进行电泳和介电泳,并按运动速度之矢量合成路径迁移。基此可在一次电泳实验中,同时测得相同条件下细胞的淌度和介电淌度~([1]),达到事半功倍的效果。所得数据更具可比性,也更适合于细胞生理、病理等研究
Electrophoresis and dielectrophoresis are two different types of electro-movement:the former forces a charged particle to migrate along the direction of the applied electric field through solution while the latter induces polarization of a particle and then forces it to migrate along a direction with varying strength of electric field.Their mechanisms are so different that they have to be performed separately.It is thus interesting if they can be performed in one shot and how.In order to have an answer,we have got some deep theoretical analysis and deduced a positive conclusion based on microfluidics.With a specially designed microfluidic chip channel structure applied with a proper electric field,we are now able to force some polarizable particles like cells to undergo simultaneous electrophoresis and dielectrophoresis,and have observed the particles migrated along a synthetic trace of the two velocity vectors.This allowed us to determine the electrophoretic and dielectrophoretic mobilities under identical conditions,suitable for no-difference comparison in the studies of cell biology and pathology.
Electrophoresis and dielectrophoresis are two different types of electro-movement:the former forces a charged particle to migrate along the direction of the applied electric field through solution while the latter induces polarization of a particle and then forces it to migrate along a direction with varying strength of electric field.Their mechanisms are so different that they have to be performed separately.It is thus interesting if they can be performed in one shot and how.In order to have an answer,we have got some deep theoretical analysis and deduced a positive conclusion based on microfluidics.With a specially designed microfluidic chip channel structure applied with a proper electric field,we are now able to force some polarizable particles like cells to undergo simultaneous electrophoresis and dielectrophoresis,and have observed the particles migrated along a synthetic trace of the two velocity vectors.This allowed us to determine the electrophoretic and dielectrophoretic mobilities under identical conditions,suitable for no-difference comparison in the studies of cell biology and pathology.
引文
[1]Zhu X.,Chen Y..Electrophoresis 2015,36:1507.