液质联用之化学增敏
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- 英文论文题名：Chemistry-sensitized Liquid chromatography-mass spectrometry
- 论文作者：陈义
- 英文论文作者：Yi Chen ; Institute of Chemistry ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院化学研究所;
- 论文关键词：色质联用 ; 离子化 ; 化学衍生 ; 植物激素 ; 超痕量分析
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：Q946.885;O657.63
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：204k
- 原文格式：D
- 会议级别：全国
摘要
液质联用是当前一种普遍应用的高效分离与鉴定分析工具,但在痕量与超痕量分析中常存在检测灵敏度不足的问题,由此引发了一系列与仪器接口相关的研究,发展了很多新颖的样品解吸和离子化方法以及离子导入技术。该类研究由于牵涉仪器装置改造,成本高、见效慢,所以另有一路人马就从化学入手,以化学的思路发展增敏方法,也取得了效果,其中以化学衍生方法最具标志性。化学衍生方法简单,成本低,见效快,但至今依然不能用来直接衍生超微量样品中的超痕量组分,面临了新分析挑战。我们就此展开了研究,发展了三类新方法,即配位移质法、表面等离子体共振增强法和超微量化学衍生法。前两法适合于用MAI-DI-TOFMS做高灵敏、高分辨测定,后一种方法比较普适,特别是能用于色质联用测定中,可衍生组分浓度低达pmol/L水平。我们藉此,实现了微克级新鲜植物样品中痕量与超痕量植物激素的测定,其空间分辨达到了花药的尺寸,能用于比较研究植物微器官中植物激素的差异。
LC-MS is nowadays a powerful analytical tool but often suffered from insufficient sensitivity in measuring trace and ultra-trace samples.Although new ionization interfaces are potential route to overcome the issue,chemical strategies are more straightforward and attractive in respect of cost and easiness.Nevertheless,the present chemical methods such as chemical labeling cannot directly treat the ultra-trace substances in micro samples.Three innovative approaches have thus been explored,that is,complex-based mass shift,surface-plasmon-resonance-enhanced desorption and ionization,and pM-labeling technique.The first two were shown to be suitable for MALDI-TOF MS of small molecules while the third to be universal,especially suitable for LC-MS.In the analysis of plant hormones such as gibberellins,it could directly label pM analytes in only μg level of fresh plant samples.This made it possible to determine the plant hormones in a single stamen or even its anther.
LC-MS is nowadays a powerful analytical tool but often suffered from insufficient sensitivity in measuring trace and ultra-trace samples.Although new ionization interfaces are potential route to overcome the issue,chemical strategies are more straightforward and attractive in respect of cost and easiness.Nevertheless,the present chemical methods such as chemical labeling cannot directly treat the ultra-trace substances in micro samples.Three innovative approaches have thus been explored,that is,complex-based mass shift,surface-plasmon-resonance-enhanced desorption and ionization,and pM-labeling technique.The first two were shown to be suitable for MALDI-TOF MS of small molecules while the third to be universal,especially suitable for LC-MS.In the analysis of plant hormones such as gibberellins,it could directly label pM analytes in only μg level of fresh plant samples.This made it possible to determine the plant hormones in a single stamen or even its anther.
引文
[1]Li D.,Guo Z.,Chen Y..Molecular Plant,2016,9:175