不同ELISA试剂盒检测牛结核效果比较
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- 英文论文题名：COMPARATIVE EFFICACY OF DIFFERENT ELISA KITS FOR DETECTION OF BOVINE γ-IFN
- 论文作者：邵红霞 ; 赵巍 ; 黄健 ; 钱琨 ; 叶建强 ; 秦爱建
- 英文论文作者：SHAO Hong-xia ; ZHAO Wei ; WANG Jian ; QIAN Kun ; YE Jianq-iang ; QIN Ai-jian ; Ministry of Education Key Laboratory for Avian Preventive Medicine ; and Key Laboratory of Jiangsu Preventive Veterinary Medicine ; Yangzhou University ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
- 年：2016
- 作者机构：禽类预防医学教育部重点实验室江苏省动物预防医学重点实验室扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;
- 论文关键词：检测牛γ干扰素 ; 自制的双夹心ELISA试剂盒 ; 商品化试剂盒 ; 比较
- 英文论文关键词：Detection of Bovine γ-IFN ; Domestic sandwich ELISA ; Commercial ELISA ; Comparison
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：中文
- 分类号：S858.23
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：8
- 文件大小：646k
- 原文格式：D
- 会议级别：全国
摘要
为了研制高质量牛结核检测试剂盒,本研究对自行研制的检测牛γ干扰素的双夹心ELISA试剂盒与商品化试剂盒进行了比较。用自行研制的双夹心ELISA试剂盒对1255份经牛PPD刺激的牛全血培养上清样品进行了检测,结果发现94份阳性样品,检测阳性率为7.5%。同时用进口的ELISA试剂盒对其中的472份样品进行了对比检测,两者阳性符合率为97.6%,阴性符合率为96.4%,总符合率为96.6%。这一比较检测结果证明,自行研制的检测牛γ干扰素的双夹心ELISA试剂盒有很好的应用前景。
To generate high quality detection kit for Bovine Tuberculosis,the comparative analysis of the domestic sandwich ELISA developed in our laboratoryand the commercial ELISA for detection of Bovine γ-IFN was performed.In 1255 samples of the supernatants from the culture of the bovine whole blood stimulated with BPPD,94 samples were positive and the positive rate was 7.5%in the domestic sandwich ELISA,472 of 1255 samples were tested by both ELISA kits.Our data showed that the positive,negative and total compatibility between the two ELISAs were 97.6%,96.4% and 96.6% respectively.This comparative detection demonstrated that the domestic sandwich ELISA developed in our laboratory had an applaud application for detection of Bovine γ-IFN.
To generate high quality detection kit for Bovine Tuberculosis,the comparative analysis of the domestic sandwich ELISA developed in our laboratoryand the commercial ELISA for detection of Bovine γ-IFN was performed.In 1255 samples of the supernatants from the culture of the bovine whole blood stimulated with BPPD,94 samples were positive and the positive rate was 7.5%in the domestic sandwich ELISA,472 of 1255 samples were tested by both ELISA kits.Our data showed that the positive,negative and total compatibility between the two ELISAs were 97.6%,96.4% and 96.6% respectively.This comparative detection demonstrated that the domestic sandwich ELISA developed in our laboratory had an applaud application for detection of Bovine γ-IFN.
引文
[1]刘思国,于辉.牛结核病研究进展[J].畜牧兽医科技信息,2003,19(10):10-14.
[2]Humblet,M.F.,M.L.Boschiroli,and C.Saegerman.Classification of worldwide bovine tuberculosis risk factors in cattle:a stratified approach[J].Vet Res,2009,40(5):50.
[3]黄大卢.Bo IFN-γ基因的原核表达及其单克隆抗体的研制[D].扬州大学硕士学位论文,2012.
[4]黄健.牛结核病诊断方法的建立及初步应用[D].扬州大学硕士学位论文,2015.
[5]Wood P R,Corner L A,Placjett P.Development of a simple,rapid in vitro cellular assay for bovine tuberculosis based on the production of gamma interferon[J].Res Vet Sic,1990,49:46-49.
[6]袁群芳.IFN-γ体外释放法检测牛结核病[J].中国动物保健,2014,16(7):32-35.
[7]Michel Denis D,Wedlock N,Allison R M,et al.Enhancement of the sensitivity of the whole-blood gamma interferon assay for diagnosis of Mycobacterium bovis infections in cattle[J].Clin Vaccine Immunol,2007,14(11):1483-1489.
[8]Rua-Domenech R,Goodchild A T.Antemortem diagnosis of tuberculosis in cattle:a review of the tuberculin tests,gamma-interferon assay and other ancillary diagnostic techniques[J].Res Vet Sci,2006,81(2):190-210.
[9]RM Sarnaik,M Sharma,SK Kate,SK Jindal.Serodiagnosis of tuberculosis:assessment of kaolin agglutination test[J].Tuber Lung Dis,1993,74(6):405-406.
[10]陈祥.γ-干扰素试验和皮试变态反应对检测奶牛结核病的比较[J].中国人兽共患病学报,2011,27(2),97-100.
[11]Emmerzaal A,Deleu S.Cattle tuberculosis:caused by Mycobacterium bovine is difficult to detect and to treat[J].Veehouderen Dierenarts,2000,14(2):4-7.
[2]Humblet,M.F.,M.L.Boschiroli,and C.Saegerman.Classification of worldwide bovine tuberculosis risk factors in cattle:a stratified approach[J].Vet Res,2009,40(5):50.
[3]黄大卢.Bo IFN-γ基因的原核表达及其单克隆抗体的研制[D].扬州大学硕士学位论文,2012.
[4]黄健.牛结核病诊断方法的建立及初步应用[D].扬州大学硕士学位论文,2015.
[5]Wood P R,Corner L A,Placjett P.Development of a simple,rapid in vitro cellular assay for bovine tuberculosis based on the production of gamma interferon[J].Res Vet Sic,1990,49:46-49.
[6]袁群芳.IFN-γ体外释放法检测牛结核病[J].中国动物保健,2014,16(7):32-35.
[7]Michel Denis D,Wedlock N,Allison R M,et al.Enhancement of the sensitivity of the whole-blood gamma interferon assay for diagnosis of Mycobacterium bovis infections in cattle[J].Clin Vaccine Immunol,2007,14(11):1483-1489.
[8]Rua-Domenech R,Goodchild A T.Antemortem diagnosis of tuberculosis in cattle:a review of the tuberculin tests,gamma-interferon assay and other ancillary diagnostic techniques[J].Res Vet Sci,2006,81(2):190-210.
[9]RM Sarnaik,M Sharma,SK Kate,SK Jindal.Serodiagnosis of tuberculosis:assessment of kaolin agglutination test[J].Tuber Lung Dis,1993,74(6):405-406.
[10]陈祥.γ-干扰素试验和皮试变态反应对检测奶牛结核病的比较[J].中国人兽共患病学报,2011,27(2),97-100.
[11]Emmerzaal A,Deleu S.Cattle tuberculosis:caused by Mycobacterium bovine is difficult to detect and to treat[J].Veehouderen Dierenarts,2000,14(2):4-7.