PBD-2转基因猪抗猪传染性胸膜肺炎的研究
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- 英文论文题名：Overexpression of porcine beta-defensin 2 enhances resistance to Actinobacillus pleuropneumoniae infection in pigs
- 论文作者：杨希 ; 周锐
- 英文论文作者：Yang xi ; Zhou rui ; State Key Laboratory of Agricultural Microbiology ; College of Veterinary Medicine ; Huazhong Agricultural University ; ChongQing Academy of Animal Sciences
- 年：2016
- 作者机构：华中农业大学动物医学院;重庆市畜牧科学院畜牧工程所;
- 论文关键词：转基因猪 ; 猪β防御素2 ; 传染性胸膜肺炎防线杆菌
- 英文论文关键词：transgenic pig ; porcine beta defensin 2 ; Actinobacillus pleuropneumoniae
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：中文
- 分类号：S858.28
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：6
- 文件大小：1596k
- 原文格式：O
- 会议级别：全国
摘要
(目的)为了减少抗生素在动物生产中的需求,需要运用新的方法来减少养殖中疾病的发生。本研究旨在探究是否能通过在猪体内过量表达猪β防御素2(PBD-2),从而提高机体对细菌感染的抵抗力。(方法)本研究利用手工克隆的方法获得PBD-2转基因猪,通过直接气管内注射法或同居感染法感染胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae),比较转基因猪和野生型猪对临床症状、病理变化和肺组织载菌量。(结果)结果表明,PBD-2稳定转染细胞株培养上清有明显的抑菌圈;共获得7头过量表达PBD-2的转基因猪,PBD-2在转基因猪心、肝、脾、肺和肠道中高量表达,显著减少了肺组织的载菌量,延缓死亡时间,减少肺部损伤。(结论)结果提示,在猪体内过量表达PBD-2能增强其对A.pleuropneumoniae感染的抵抗力。
To reduce the need of antibiotics in animal production,alternative approaches are needed to control infection.We hypothesized that overexpression of native defensin genes will provide food animals with enhanced resistance to bacterial infections.In this study,recombinant porcine beta-defensin 2(PBD-2) was overexpressed in stably transfected PK-15 porcine kidney cells.PBD-2 antibacterial activities were evaluated on agar plates against Actinobacillus pleuropneumoniae,an important respiratory pathogen causing porcine contagious pleuropneumonia.Transgenic pigs constitutively overexpressing PBD-2 were produced by somatic cell cloning method,and their resistance to bacterial infection was evaluated by direct or cohabitation infection with A.pleuropneumoniae.PBD-2 was overexpressed in the heart,liver,spleen,lungs,kidneys,and jejunum of the transgenic pigs,which showed significantly lower bacterial loads in the lungs,and reduced lung lesions after direct or cohabitation infection with A.pleuro pneumoniae.The results demonstrate that transgenic overexpression of PBD-2 in pigs confers enhanced resistance against A.pleuropneumoniae infection.
To reduce the need of antibiotics in animal production,alternative approaches are needed to control infection.We hypothesized that overexpression of native defensin genes will provide food animals with enhanced resistance to bacterial infections.In this study,recombinant porcine beta-defensin 2(PBD-2) was overexpressed in stably transfected PK-15 porcine kidney cells.PBD-2 antibacterial activities were evaluated on agar plates against Actinobacillus pleuropneumoniae,an important respiratory pathogen causing porcine contagious pleuropneumonia.Transgenic pigs constitutively overexpressing PBD-2 were produced by somatic cell cloning method,and their resistance to bacterial infection was evaluated by direct or cohabitation infection with A.pleuropneumoniae.PBD-2 was overexpressed in the heart,liver,spleen,lungs,kidneys,and jejunum of the transgenic pigs,which showed significantly lower bacterial loads in the lungs,and reduced lung lesions after direct or cohabitation infection with A.pleuro pneumoniae.The results demonstrate that transgenic overexpression of PBD-2 in pigs confers enhanced resistance against A.pleuropneumoniae infection.
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