Genome sequence of the edible mushroom Lentinula edodes(shiitake) reveals insights into Lignocellulose degradation
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- 英文论文题名：Genome sequence of the edible mushroom Lentinula edodes(shiitake) reveals insights into Lignocellulose degradation
- 论文作者：Lianfu Chen ; Yuhua Gong ; Yingli Cai ; Wei Liu ; Yan zhou ; Yang Xiao ; Zhangyi Xu ; Yin Liu ; Xiaoyu Lei ; Gangzheng Wang ; Mengpei Guo ; Xiaolong Ma ; Yinbing Bian
- 英文论文作者：Lianfu Chen ; Yuhua Gong ; Yingli Cai ; Wei Liu ; Yan zhou ; Yang Xiao ; Zhangyi Xu ; Yin Liu ; Xiaoyu Lei ; Gangzheng Wang ; Mengpei Guo ; Xiaolong Ma ; Yinbing Bian ; Institute of Applied Mycology ; Plant Science and Technology College ; Huazhong Agricultural University ; Key Laboratory of Agro-Microbial Resource Comprehensive Utilization ; Ministry of Agriculture ; Huazhong Agricultural University ; Food Science and Technology College ; Huazhong Agricultural University
- 年：2016
- 作者机构：Institute of Applied Mycology,Plant Science and Technology College,Huazhong Agricultural University;Key Laboratory of Agro-Microbial Resource Comprehensive Utilization,Ministry of Agriculture,Huazhong Agricultural University;Food Science and Technology College,Huazhong Agricultural University;
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：S646.12
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：2227k
- 原文格式：D
- 会议级别：全国
摘要
Lentinula edodes,one of the most popular edible mushroom species with high nutrition and special flavor,is widely cultivated in many Asian countries,especially in China,Japan and Korea.As a white rot fungus with strong wood degradation ability,L.edodes has the potential application in bioenergy production.However,genes,pathways and mechanisms regarding this function are still unknown.Here,we report its 41.8-Mb genome,encoding 14,889 predicted genes,obtained via next-generation sequencing technology.L.edodes' phylogenies and wooddegrading machinery were investigated by a comparative analysis with the model species of fungi.A total of 93 genes predicted to encode transcription factors and 41 genes related to lignocellulose degradation are up-regulated when L edodes mycelia were cultured in sawdust medium versus CYM medium.These differentially expressed genes may play important roles in lignocellulose degradation,and the research of these genes may be beneficial to bioenergy industry,environmental improvement and mushroom production.The overall results will facilitate a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L.edodes cultivation.
Lentinula edodes,one of the most popular edible mushroom species with high nutrition and special flavor,is widely cultivated in many Asian countries,especially in China,Japan and Korea.As a white rot fungus with strong wood degradation ability,L.edodes has the potential application in bioenergy production.However,genes,pathways and mechanisms regarding this function are still unknown.Here,we report its 41.8-Mb genome,encoding 14,889 predicted genes,obtained via next-generation sequencing technology.L.edodes' phylogenies and wooddegrading machinery were investigated by a comparative analysis with the model species of fungi.A total of 93 genes predicted to encode transcription factors and 41 genes related to lignocellulose degradation are up-regulated when L edodes mycelia were cultured in sawdust medium versus CYM medium.These differentially expressed genes may play important roles in lignocellulose degradation,and the research of these genes may be beneficial to bioenergy industry,environmental improvement and mushroom production.The overall results will facilitate a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L.edodes cultivation.
Lentinula edodes,one of the most popular edible mushroom species with high nutrition and special flavor,is widely cultivated in many Asian countries,especially in China,Japan and Korea.As a white rot fungus with strong wood degradation ability,L.edodes has the potential application in bioenergy production.However,genes,pathways and mechanisms regarding this function are still unknown.Here,we report its 41.8-Mb genome,encoding 14,889 predicted genes,obtained via next-generation sequencing technology.L.edodes' phylogenies and wooddegrading machinery were investigated by a comparative analysis with the model species of fungi.A total of 93 genes predicted to encode transcription factors and 41 genes related to lignocellulose degradation are up-regulated when L edodes mycelia were cultured in sawdust medium versus CYM medium.These differentially expressed genes may play important roles in lignocellulose degradation,and the research of these genes may be beneficial to bioenergy industry,environmental improvement and mushroom production.The overall results will facilitate a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L.edodes cultivation.
引文
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Martin F et al.Nature 2008,452(7183):88-92.
Martinez D et al.Nat Biotech 2004,22(6):695-700.
Ohm RA et al.Nature biotechnology 2010.28(9):957-963.
Morin E et al.Proceedings of the National Academy of Sciences 2012.109(43):17501-17506.
Stajich JE et al.Proceedings of the National Academy of Sciences 2010,107(26):11889-11894.
Gnerre S et al.Proceedings of the National Academy of Sciences 2011,108(4):1513-1518.
Yin Y et al.Nucleic acids research 2012,40(W1):W445-W451.
Chen S et al.Nature communications 2012,3:913.
Haas BJ et al.Mycology 2011,2(3):118-141.
Tang L-h et al.Applied microbiology and biotechnology 2013,97(11}:4977-4989.
Au Ch et al.Gene 2013(0).
Wu L et al.Gene 2013,531(2):270-273.
Chen B et al.PLoS ONE 2013,8(3):e58780.
Alananbeh KM et al.Saudi Journal of Biological Sciences 2014,21(6):616-625.