RNA-Seq identifies genes associated with aberrant meiotic prophase I of a novel TE5A genic male sterility in Brassica napus
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- 英文论文题名：RNA-Seq identifies genes associated with aberrant meiotic prophase I of a novel TE5A genic male sterility in Brassica napus
- 论文作者：Xiaohong Yan ; Xinhua Zeng ; Gang Wu
- 英文论文作者：Xiaohong Yan ; Xinhua Zeng ; Gang Wu ; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences ; Key Laboratory of Biology and Genetic Improvement of Oil Crops ; Ministry of Agriculture
- 年：2016
- 作者机构：Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture;
- 英文论文关键词：genic male sterility ; RNA-Seq ; prophase I ; meiotic cycle ; meiosis
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S565.4;Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Genic male sterility(GMS),as a promising pollination control system,has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus. Analysis of paraffin-cross sections of anthers and TEM showed that the male gamete development was arrested at meiosis prophase I,eventually,meiotic PMCs were degenerated,leaving an empty locule,and no pollen grains were generated. Chromosome spreads and FISH showed that homologous chromosomes could not pair,synapse,condense and form bivalents in TE5A. Ed U uptake of S-phase meiocytes revealed that TE5A mutant could accomplish DNA replication. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants. RNA-Seq analysis identified 3,841 differentially expressed genes(DEGs),some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. These DEGs represented a set of potential candidate genes associated with GMS in the TE5A. Dynamic expression changes of candidate DEGs were then detected at different developmental stages of anthers. The present study provided a global assessment of the differences between GMS sterile plants and its near-isogenic fertile plants,as well as identified new fertility-associated genes and elucidated the mechanisms of GMS.
Genic male sterility(GMS),as a promising pollination control system,has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus. Analysis of paraffin-cross sections of anthers and TEM showed that the male gamete development was arrested at meiosis prophase I,eventually,meiotic PMCs were degenerated,leaving an empty locule,and no pollen grains were generated. Chromosome spreads and FISH showed that homologous chromosomes could not pair,synapse,condense and form bivalents in TE5A. Ed U uptake of S-phase meiocytes revealed that TE5A mutant could accomplish DNA replication. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants. RNA-Seq analysis identified 3,841 differentially expressed genes(DEGs),some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. These DEGs represented a set of potential candidate genes associated with GMS in the TE5A. Dynamic expression changes of candidate DEGs were then detected at different developmental stages of anthers. The present study provided a global assessment of the differences between GMS sterile plants and its near-isogenic fertile plants,as well as identified new fertility-associated genes and elucidated the mechanisms of GMS.
Genic male sterility(GMS),as a promising pollination control system,has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus. Analysis of paraffin-cross sections of anthers and TEM showed that the male gamete development was arrested at meiosis prophase I,eventually,meiotic PMCs were degenerated,leaving an empty locule,and no pollen grains were generated. Chromosome spreads and FISH showed that homologous chromosomes could not pair,synapse,condense and form bivalents in TE5A. Ed U uptake of S-phase meiocytes revealed that TE5A mutant could accomplish DNA replication. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants. RNA-Seq analysis identified 3,841 differentially expressed genes(DEGs),some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. These DEGs represented a set of potential candidate genes associated with GMS in the TE5A. Dynamic expression changes of candidate DEGs were then detected at different developmental stages of anthers. The present study provided a global assessment of the differences between GMS sterile plants and its near-isogenic fertile plants,as well as identified new fertility-associated genes and elucidated the mechanisms of GMS.
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