Study on distribution and characterization of Aspergillus flavus strains to screen potential biocontrol agents from different geographical locations of China
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- 英文论文题名：Study on distribution and characterization of Aspergillus flavus strains to screen potential biocontrol agents from different geographical locations of China
- 论文作者：Firew Tafesse Mamo ; Bo Shang ; Jonathan Nimal Selvaraj ; Yan Wang ; Yang Liu
- 英文论文作者：Firew Tafesse Mamo ; Bo Shang ; Jonathan Nimal Selvaraj ; Yan Wang ; Yang Liu ; Institute of Food Science and Technology ; Chinese Academy of Agricultural Sciences/Key Laboratory of Agroproducts Processing ; Ministry of Agriculture
- 年：2016
- 作者机构：Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences/Key Laboratory of Agroproducts Processing,Ministry of Agriculture;
- 英文论文关键词：Aflatoxin ; Cyclopiazonic acid(CPA) ; Aspergillus flavus ; atoxigenic strain ; toxigenic strains ; biocontrol
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：S476
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：675k
- 原文格式：D
- 会议级别：全国
摘要
Aflatoxin contamination of crops like peanut,maize and rice is still a major public health and economic problem for the world,the impact is more significant for developing countries.One promising method for combating aflatoxin formation is biocontrol using competitive exclusion,currently under intensive study in peanut crops in different parts of China.Potential A.flavus strains as a biocontrol must be unable to produce both aflatoxin and cyclopiazonic acids and also must incapable to convert to toxigenic.In the current study a total of 724 Aspergillus flavus strains isolated from peanut,maize and rice in 21 geographical locations of China were screened.Of these,229 strains did not produce aflatoxins.The incidence of atoxigenic A.flavus strains range from 28-35% from three crops.Aflatoxin productions in A.flavus isolates were varied significantly among all sampled locations(X220,0.05= 0.012).Non toxigenic(non AFB1 and CPA) strains were screened for their potential as biocontrol by examining the 28 aflatoxin biosynthesis genes,using a PCR assay.Strains which do not have afl R PCR products were selected for analysis of the other 27 biosynthetic genes.Twenty four strains showed failure,in the,fas-2 and afl J genes,too.Sixteen(67%) of atoxigenic A.flavus strains failed to show a PCR product in 10 or more genes.Altogether,18 PCR product patterns were observed,usually common to only one or two isolates,exceptionally six of stains have the same(K) deletion pattern,indicating great diversity in the aflatoxin biosynthesis pathway in A.flavus isolates taken from different crops and locations.The extensive deletions of 10 or more genes in the aflatoxin gene cluster identified in candidate's nonaflatoxigenic biocontrol A.flavus isolates serve as a safeguard in preventing adverse genetic reversion,thereby ensuring their safe application to agricultural fields.Two genes dma T and mao A could be used a marker genes for CPA production capacity atoxigenic stains.Biological control of aflatoxin contamination by use of atoxigenic strains could be therefore widely tested and adapted allover potential agricultural regions of China.
Aflatoxin contamination of crops like peanut,maize and rice is still a major public health and economic problem for the world,the impact is more significant for developing countries.One promising method for combating aflatoxin formation is biocontrol using competitive exclusion,currently under intensive study in peanut crops in different parts of China.Potential A.flavus strains as a biocontrol must be unable to produce both aflatoxin and cyclopiazonic acids and also must incapable to convert to toxigenic.In the current study a total of 724 Aspergillus flavus strains isolated from peanut,maize and rice in 21 geographical locations of China were screened.Of these,229 strains did not produce aflatoxins.The incidence of atoxigenic A.flavus strains range from 28-35% from three crops.Aflatoxin productions in A.flavus isolates were varied significantly among all sampled locations(X220,0.05= 0.012).Non toxigenic(non AFB1 and CPA) strains were screened for their potential as biocontrol by examining the 28 aflatoxin biosynthesis genes,using a PCR assay.Strains which do not have afl R PCR products were selected for analysis of the other 27 biosynthetic genes.Twenty four strains showed failure,in the,fas-2 and afl J genes,too.Sixteen(67%) of atoxigenic A.flavus strains failed to show a PCR product in 10 or more genes.Altogether,18 PCR product patterns were observed,usually common to only one or two isolates,exceptionally six of stains have the same(K) deletion pattern,indicating great diversity in the aflatoxin biosynthesis pathway in A.flavus isolates taken from different crops and locations.The extensive deletions of 10 or more genes in the aflatoxin gene cluster identified in candidate's nonaflatoxigenic biocontrol A.flavus isolates serve as a safeguard in preventing adverse genetic reversion,thereby ensuring their safe application to agricultural fields.Two genes dma T and mao A could be used a marker genes for CPA production capacity atoxigenic stains.Biological control of aflatoxin contamination by use of atoxigenic strains could be therefore widely tested and adapted allover potential agricultural regions of China.
Aflatoxin contamination of crops like peanut,maize and rice is still a major public health and economic problem for the world,the impact is more significant for developing countries.One promising method for combating aflatoxin formation is biocontrol using competitive exclusion,currently under intensive study in peanut crops in different parts of China.Potential A.flavus strains as a biocontrol must be unable to produce both aflatoxin and cyclopiazonic acids and also must incapable to convert to toxigenic.In the current study a total of 724 Aspergillus flavus strains isolated from peanut,maize and rice in 21 geographical locations of China were screened.Of these,229 strains did not produce aflatoxins.The incidence of atoxigenic A.flavus strains range from 28-35% from three crops.Aflatoxin productions in A.flavus isolates were varied significantly among all sampled locations(X220,0.05= 0.012).Non toxigenic(non AFB1 and CPA) strains were screened for their potential as biocontrol by examining the 28 aflatoxin biosynthesis genes,using a PCR assay.Strains which do not have afl R PCR products were selected for analysis of the other 27 biosynthetic genes.Twenty four strains showed failure,in the,fas-2 and afl J genes,too.Sixteen(67%) of atoxigenic A.flavus strains failed to show a PCR product in 10 or more genes.Altogether,18 PCR product patterns were observed,usually common to only one or two isolates,exceptionally six of stains have the same(K) deletion pattern,indicating great diversity in the aflatoxin biosynthesis pathway in A.flavus isolates taken from different crops and locations.The extensive deletions of 10 or more genes in the aflatoxin gene cluster identified in candidate's nonaflatoxigenic biocontrol A.flavus isolates serve as a safeguard in preventing adverse genetic reversion,thereby ensuring their safe application to agricultural fields.Two genes dma T and mao A could be used a marker genes for CPA production capacity atoxigenic stains.Biological control of aflatoxin contamination by use of atoxigenic strains could be therefore widely tested and adapted allover potential agricultural regions of China.
引文
Donner,M.,Atehnkeng,J.,Sikora,R.a,Bandyopadhyay,R.,&Cotty,P.J,2010.Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria.Food Additives&Contaminants.Part A,Chemistry,Analysis,Control,Exposure&Risk Assessment,27:576-590.
Horn,B.W.,&Dorner,J.W,2011.Evaluation of different genotypes of nontoxigenic Aspergillus flavus for their ability to reduce aflatoxin contamination in peanuts.Biocontrol Science and Technology,21:865-876.
Mauro,A.,Battilani,P.,&Cotty,P.J,2014.Atoxigenic Aspergillus flavus endemic to Italy for biocontrol of aflatoxins in maize.Bio Control,60:125-134.
Saito,M.,&Machida,S,1999.A rapid identification method for aflatoxin-producing strains of Aspergillus flavus and A.parasiticus by ammonia vapor.Mycoscience,40(2):205-208.
Gao,J.,Liu,Z.,&Yu,J,2007.Identification of Aspergillus section Flavi in maize in northeastern China.Mycopathologia,164:91-95.
Horn,B.W.,&Dorner,J.W,2011.Evaluation of different genotypes of nontoxigenic Aspergillus flavus for their ability to reduce aflatoxin contamination in peanuts.Biocontrol Science and Technology,21:865-876.
Mauro,A.,Battilani,P.,&Cotty,P.J,2014.Atoxigenic Aspergillus flavus endemic to Italy for biocontrol of aflatoxins in maize.Bio Control,60:125-134.
Saito,M.,&Machida,S,1999.A rapid identification method for aflatoxin-producing strains of Aspergillus flavus and A.parasiticus by ammonia vapor.Mycoscience,40(2):205-208.
Gao,J.,Liu,Z.,&Yu,J,2007.Identification of Aspergillus section Flavi in maize in northeastern China.Mycopathologia,164:91-95.