超高效液相色谱串联质谱法测定大气颗粒物中脱水单糖

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• 英文论文题名：Determination of Monosaccharide Anhydrides in Atmospheric Particles by Ultrasonic Extraction Coupled with Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
• 论文作者：赵起越 ; 赵红帅 ; 刘保献 ; 王焱 ; 袁汉成
• 英文论文作者：Zhao Qiyue ; Zhao Honghuai ; Liu Baoxian ; Wang Yan ; Yuan Hancheng ; Beijing Municipal Environmental Monitoring Center ; Beijing Key Laboratory of Airborne Particulate Matter Monitoring Technology ; Waters Sci.& Technol. Co. Ltd.(Shanghai)
• 年：2016
• 作者机构：北京市环境保护监测中心大气颗粒物监测技术北京市重点实验室;沃特世科技(上海)有限公司;
• 论文关键词：超声萃取 ; 超高效液相色谱-串联质谱 ; 大气颗粒物 ; 左旋葡聚糖 ; 异构体
• 英文论文关键词：ultrasonic extraction ; ultra-high performance liquid chromatography-tandem mass spectrometry ; atmospheric particles ; levoglucosan ; isomers
• 会议召开时间：2016-09-22
• 会议录名称：2016年中国环境与安全监测技术研讨会—第27届MICONEX2016科学仪器惠及民生系列分会场论文集
• 语种：中文
• 分类号：X831
• 学会代码：XDKX
• 会议名称：2016年中国环境与安全监测技术研讨会—第27届MICONEX2016科学仪器惠及民生系列分会场
• 会议地点：中国北京
• 主办单位：中国仪器仪表学会
• 学会名称：现代科学仪器编辑部
• 页数：6
• 文件大小：2472k
• 原文格式：D
• 会议级别：全国

摘要

采用超声萃取与超高效液相色谱-串联质谱联用,建立了测定大气颗粒物中左旋葡聚糖及其异构体——甘露聚糖及半乳聚糖的方法。颗粒物样品用甲醇超声萃取,浓缩后使用超高效液相色谱-串联质谱分析。采用电喷雾电离方式,对色谱及质谱条件进行优化,对超声萃取条件进行筛选,并进行实际样品测定。在优化的多反应监测模式下,方法回收率在80.5%----92.2%之间,检出限左旋葡聚糖为2.0ng/m L,甘露聚糖及半乳聚糖为3.0ng/m L。相对标准偏差15%以下。相比气相色谱-质谱联用分析左旋葡聚糖及异构体的方法,本方法不用衍生,节省了人力、物力,更快速、经济、灵敏,实际样品的测试证明本方法可以满足大气颗粒物中此类化合物的定量分析要求。
In order to do source appointment of atmospheric particles, an analytical method was developed for determination of monosaccharide anhydrides(levoglucosan and its 2 isomers) in atmospheric particles by ultrasonic extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS /MS). After extraction by methanol and enrichment, isomers were separated on a BEH Amide C18 column(100mm×2.1mm, 1.7μm), with acetonitrile and water served as mobile phase in gradient elution, then determined by UPLC-MS/MS using electrospray negative ionization(ESI-) and multiple reaction monitoring(MRM). Parameters of chromatography and mass spectrometry, as well as conditions of ultrasonic extraction were optimized through experiments. The developed method provided good linearities(r2>0.99) and recoveries(80.5%—92.2%) for 3 isomers. Low detection limit for levoglucosanis 2.0ng/m L, mannosan and galactosanis 3.0ng/m L respectively, RSD is less than 15%(n =6). Comparing with GC/MS, this method is more rapid, sensitive and economical because derivation is eliminated,itsaves human and material resources. Samples of atmospheric particles are detected using this method,it can meet the requirements of quantitative analysis of monosaccharide anhydrides in atmosphere.

引文

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