LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
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- 英文论文题名：LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
- 论文作者：陈龙 ; Huixin Yang ; Yanbin Xiao ; Xiaoxia Tang ; Yuqian Li ; Qiaoqiao Han ; Junping Fu ; Yuye Yang ; Yuechun Zhu
- 英文论文作者：Long Chen ; Huixin Yang ; Yanbin Xiao ; Xiaoxia Tang ; Yuqian Li ; Qiaoqiao Han ; Junping Fu ; Yuye Yang ; Yuechun Zhu ; Department of Biochemistry and Molecular Biology of Kunming Medical University ; PET/CT Center ; Yunan Tumor Hospital/the Third Affiliated Hospital of Kunming Medical University ; Department of Orthopaedics ; Yunan Tumor Hospital/the Third Affiliated Hospital of Kunming Medical University ; Department of Pharmacy ; the Second Affiliated Hospital of Kunming Medical University
- 年：2016
- 作者机构：昆明医科大学生物化学与分子生物学系;PET/CT Center,Yunnan Tumor Hospital/the Third Affiliated Hospital of Kunming Medical University;Department of Orthopaedics,Yunnan Tumor Hospital/the Third Affiliated Hospital of Kunming Medical University;Department of Pharmacy,the Second Affiliated Hospital of Kunming Medical University;
- 英文论文关键词：lncRNA ; GAS5 ; melanoma ; metastasis ; gelatinase
- 会议召开时间：2016-09-25
- 会议录名称：第九届全国核糖核酸学术讨论会论文集
- 语种：英文
- 分类号：R739.5
- 学会代码：IGSS
- 会议名称：第九届全国核糖核酸学术讨论会
- 会议地点：中国上海
- 主办单位：中国生物化学与分子生物学会核糖核酸专业委员会
- 学会名称：中国生物化学与分子生物学会
- 页数：1
- 文件大小：386k
- 原文格式：D
- 会议级别：全国
摘要
The present study intended to demonstrate the effects of lncRNA Growth Arrest Specific transcript 5(GAS5)on the migration and invasion of melanoma cells.We firstly detected the GAS5 expression among four kinds of melanoma cell lines,followed by constructing GAS5-knocked down and over-expressed stable cells.Next,we evaluated the effects of GAS5 on cell migration and invasion by wound healing and the gelatin zymography assay.Finally,the nude mice were injected into melanoma cells with different GAS5 expression and the tumor volumes were recorded as well as the tumor tissues were analysed after being sacrificed.This study systematically examined the GAS5 function in mediating malanoma metsatsis and revealed that GAS5 plays an anti-cancer role in melanoma via regulating the gelatinase A and B both in vitro and in vivo.
The present study intended to demonstrate the effects of lncRNA Growth Arrest Specific transcript 5(GAS5) on the migration and invasion of melanoma cells.We firstly detected the GAS5 expression among four kinds of melanoma cell lines,followed by constructing GAS5-knocked down and over-expressed stable cells.Next,we evaluated the effects of GAS5 on cell migration and invasion by wound healing and the gelatin zymography assay.Finally,the nude mice were injected into melanoma cells with different GAS5 expression and the tumor volumes were recorded as well as the tumor tissues were analysed after being sacrificed.This study systematically examined the GAS5 function in mediating malanoma metsatsis and revealed that GAS5 plays an anti-cancer role in melanoma via regulating the gelatinase A and B both in vitro and in vivo.
The present study intended to demonstrate the effects of lncRNA Growth Arrest Specific transcript 5(GAS5) on the migration and invasion of melanoma cells.We firstly detected the GAS5 expression among four kinds of melanoma cell lines,followed by constructing GAS5-knocked down and over-expressed stable cells.Next,we evaluated the effects of GAS5 on cell migration and invasion by wound healing and the gelatin zymography assay.Finally,the nude mice were injected into melanoma cells with different GAS5 expression and the tumor volumes were recorded as well as the tumor tissues were analysed after being sacrificed.This study systematically examined the GAS5 function in mediating malanoma metsatsis and revealed that GAS5 plays an anti-cancer role in melanoma via regulating the gelatinase A and B both in vitro and in vivo.
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