云南玛咖SDE法及回流提取法提取挥发性成分GC-MS分析

详细信息    查看官网全文

• 英文论文题名：GC-MS analysis of thevolatile components of LepidiummeyeniiWalperfrom Yunnan by SDE and reflux extraction
• 论文作者：嵇政东 ; 郭翠 ; 黄靓 ; 沈丽英 ; 吕飞燕 ; 甘望宝
• 英文论文作者：Ji Zhendong ; Guo Cui ; Huang Liang ; Shen Liying ; Lv Feiyan ; Gan Wangbao ; HangMai Science And Technology(Hangzhou) Co. ; Ltd
• 年：2016
• 作者机构：杭曼科技(杭州)有限公司;
• 论文关键词：玛咖 ; SDE法回流提取法 ; GC-MS分析
• 英文论文关键词：Lepidium meyenii Walper ; SDE method ; reflux extraction ; GC-MS
• 会议召开时间：2016-06-15
• 会议录名称：第十一届中国香料香精学术研讨会论文集
• 英文会议录名称：The Proceedings of the 11th Seminar on Fragrance and Flavor China
• 语种：中文
• 分类号：O657.63;TQ651
• 学会代码：ZGXE
• 会议名称：第十一届中国香料香精学术研讨会
• 会议地点：中国上海
• 主办单位：中国香料香精化妆品工业协会
• 学会名称：中国香料香精化妆品工业协会
• 页数：7
• 文件大小：548k
• 原文格式：O
• 会议级别：全国

摘要

以我国云南种植的玛咖块根切片粉碎而成的玛咖粉为原料,通过同时蒸馏萃取(SDE)法和回流提取法提取玛咖中的挥发性香气成分,并采用GC-MS法测定了玛咖中的挥发性香气成分,共鉴定出79种化合物。其中异硫氰酸苄酯、4-甲氧基苄基异硫氰酸酯、苯乙腈、3-甲氧基苯乙腈为玛咖香气主要成分,具有玛咖特征性香气。苯乙腈单体经GC-MS单独进样分析,通过保留时间数据确认了其在玛咖提取物中的确存在。其生成机理有待进一步探究。而苯乙酰胺、N-苄基甲酰胺、烟酰胺、N-苄烯丁胺、N-苄基乙酰胺等酰胺类化合物可以作为玛咖提取物主要活性成分及特征物质。
This paperreportsvolatile componentsof LepidiummeyemiWalpersextracted by simultaneous disti llation extraction and reflux extraction from powders of sliced maca roots which are cultivated in Yun nan Province.79 chemical components are analyzed and identified by gas chromatography-mass spectr ometry(GC-MS).Among these,Benzyl isothiocyanate,4-Methoxybenzyl isothiocyanate,Benzeneac etonitrile,and Benzeneacetonitrile,3-methoxy-are main aroma components of LepidiummeyemiWalpers with characteristic flavor.According to theretention time dataofseparate sample injection and analysis b y GC-MS,Benzeneacetonitrile does exsits in maca extracts.But its formation mechanism remains to b e determined.While amides such as Benzeneacetamide,N-Benzylformamide,Nicotinamide,Benzenemeth anamine,N-(phenylmethylene)-,Acetamide,N-(phenylmethyl)-are main active ingredients and character istic components in maca extracts.

引文

[1]Quiros C F,Cardenas R A.Macs[A].Hermann M,Heller JAndean.Roots and tubers:ahipa,arracacha,maca and yaco[C].Rome:IPGRI-Promoting Conservation and Use of Underutilized andNeglected Crops21,1997:175-195.
    [2]Ruizl A C,Salazar S,Aspajo N J,et al.Lepidiummeyenii(Maca)increases litter size in normal adult female mice[J].ReprodBiolEndocrinol,2005,3(1):16-21.
    [3]余龙江,金文闻.玛咖(Lepidiummeyenii)干粉的营养成分及抗疲劳作用研究[J].食品科学,2004,25(1):164-166.
    [4]Zhang Y Z,Yu L J,Ao M Z,et al.Effectof ethanol extract of LepidiummeyeniiWalp.on osteoporosis in ovariectomized rat[J].J Ethnopharmacol,2006,106(1/2):422-428.
    [5]金文闻.药食两用植物玛咖(Lepidiummeyenii)的功效物质研究.武汉:华中科技大学,PhD.2006.
    [6]Fernando EC,Alfredo G,Jose CM,et al.Macamides fromwild'Maca'LepidiummeyeniiWalpers(Brassicaceae).PhytochemLett,2014(8):145-148.
    [7]金文闻,王晴芳,李硕,等.新疆产玛咖的挥发油成分研究[J].食品科学,2009,30(12):241-245.
    [8]孟倩倩,曾晓鹰,杨叶坤,等.云南丽江栽培玛咖的挥发性成分分析[J].精细化工,2013,30(4):442-446.
    [9]Clement C,Grados D A D,Avula B,Khan I A,Mayer A C,Aguirre D D P,Manrique I,Kreuzer M.Influence of colour type and previous cultivation on secondary metabolites in hypocotyls and leaves of maca(LepidiummeyeniiWalpers).Journal of the Science of Food and Agriculture,2010,90:861-869.
    [10]VALENTOV A K,FRCEK J,UlrichováJ.Yacon(Smallanthussonchifolius)and maca(Lepidiummeyenii),traditional Andean crops asnew functional foods on the European market[J].ChemListy,2001,95(10):594-601.