Box-Behnken响应面法优化牛至挥发油减压辅助提取工艺及GC-MS成分分析与体外抑菌作用研究
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- 英文论文题名：Box-Behnken response surface method optimized vacuum extraction technology of oregano essential oil and GC-MS component analysis with bacteriostatic action research
- 论文作者：伍振峰 ; 詹娟娟 ; 谢老智 ; 王雅琪 ; 韩飞 ; 王学成 ; 万娜 ; 杨明
- 英文论文作者：WU Zhen-feng ; ZHAN Juan-juan ; XIE Lao-zhi ; WANG Ya-qi ; HAN Fei ; WANG Xue-cheng ; WAN Na ; YANG Ming ; Key Laboratory of Modern Preparation of TCM ; Ministry of Education ; Jiangxi University of Traditional Chinese Medicine ; State Key Laboratory of Innovative Drug and Efficient Energy-saving Pharmacetuical Equipment
- 年：2016
- 作者机构：江西中医药大学现代中药制剂教育部重点实验室;创新药物与高效节能降耗制药设备国家重点实验室;
- 论文关键词：牛至 ; 挥发油 ; 减压提取 ; 响应面法 ; 气相色谱-质谱联用法 ; 抑菌
- 英文论文关键词：Oregano ; Essential oil ; Decompression extraction ; Response surface method ; GC-MS ; Bacteriostatic
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R284;O657.63
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：11
- 文件大小：1389k
- 原文格式：O
- 会议级别：全国
摘要
目的:采用水蒸气减压蒸馏法提取牛至挥发油,利用Box-Behnken响应面分析法,确定了牛至挥发油的最佳提取工艺,即加水14倍,压强558mbar,加热回流4.23h,在此工艺条件下,牛至挥发油实际得率为0.9267%,与理论值较接近。并用气相色谱-质谱联用法(GC-MS)分离鉴定,比较常压和减压提取牛至精油成分的差异及所得精油对金黄色葡萄球菌和大肠杆菌的抑菌效果。结果表明,常压和减压提取所得牛至精油在成分种类和含量上都存在一定差别,抑菌实验结果显示,无论是对金黄色葡萄球菌还是大肠杆菌,减压提取所得牛至精油的抑菌效果都优于常压提取,这可能与某些抑菌成分的含量有关。
Objective Water vapor vacuum distillation method was used to extract the essential oil from Origanum vulgare L.,and Box-Behnken central composite experimental design and response surface analysis method were used to determine the optimal extracting process of volatile oil of oregano,that is,add water 14 times,pressure 558 mbar,heating reflux 4.23 h.Under these conditions,oregano essential oil actual yield was 0.9267%,and is close to the theoretical value.And composition analysis with gas chromatography-mass spectrometry(GC-MS),compare the differences of the bacteriostatic effect of oregano essential oil extracted under the condition of normal pressure and decompression.Results show that there are some differences in the type and content of the oregano essential oil composition extracted under the condition of normal pressure and decompression.And bacteriostatic experiment results show that the bacteriostatic effects of oregano essential oil extracted under the condition of reduced pressure are better than normal pressure extraction both to staphylococcus aureus and escherichia coli,this may be related to the content of certain bacteriostasis component.
Objective Water vapor vacuum distillation method was used to extract the essential oil from Origanum vulgare L.,and Box-Behnken central composite experimental design and response surface analysis method were used to determine the optimal extracting process of volatile oil of oregano,that is,add water 14 times,pressure 558 mbar,heating reflux 4.23 h.Under these conditions,oregano essential oil actual yield was 0.9267%,and is close to the theoretical value.And composition analysis with gas chromatography-mass spectrometry(GC-MS),compare the differences of the bacteriostatic effect of oregano essential oil extracted under the condition of normal pressure and decompression.Results show that there are some differences in the type and content of the oregano essential oil composition extracted under the condition of normal pressure and decompression.And bacteriostatic experiment results show that the bacteriostatic effects of oregano essential oil extracted under the condition of reduced pressure are better than normal pressure extraction both to staphylococcus aureus and escherichia coli,this may be related to the content of certain bacteriostasis component.
引文
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[9]沈灵犀,扎西次仁,耿宇鹏,等.西藏蒿属六种植物精油化学成分分析及抑菌效果[J].复旦学报(自然科学版),2010,49(1):73-80.
[2]韩飞,李瑾,潘悄悄,等.新型天然植物抗生素牛至油的研究进展[J].中国新药杂志,201 5,24(3):303-307.
[3]T.A.Misharina,E.B.Burlakova,L.D.Fatkullina,Et al.Effect of oregano essential oil on the engraftment and development of Lewis carcinoma in Fl DBA C57 black hybrid mice[J].Applied Biochemistry andMicrobiology,2013,88(4):432-436.
[4]陈华保,杨春平,吴文君,等.内生真菌Hd3菌株发酵条件优化研究[J].西南师范大学学报:自然科学版,2011,36(1):148-153.
[5]Rojas-Grau M A,Avena-Bustillos R J,Friedman M,et al.Mechanical,barrier,and antimicrobial properties of apple puree edible films containing plant essential oils[J].Journal of Agricultural and Food Chemistry,2006,54(24):9262-9267.
[6]Kim J,Marshall M K,Wei C.Antibacterial Activity of Some Essential Oil Components against Five Foodborne Pathogens[J].Journal of agricultural and food chemistry,1995,43(11):2839-2845.
[7]干信,吴士筠,高雯琪.花椒挥发油抑菌作用研究[J].食品科学,2009,30(21):128-130
[8]赵海伊,卫飞,周才琼.3种不同方法提取牛至挥发精油及体外抑菌作用研究[J].食品科学,2013,34(2):236-240.
[9]沈灵犀,扎西次仁,耿宇鹏,等.西藏蒿属六种植物精油化学成分分析及抑菌效果[J].复旦学报(自然科学版),2010,49(1):73-80.