TALENs and CRISPR/Cas9 system mediated bi-allelic and multiplegene modifications in pigs at one step
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- 英文论文题名：TALENs and CRISPR/Cas9 system mediated bi-allelic and multiplegene modifications in pigs at one step
- 论文作者：Yao jing ; Cao chunwei ; Huang jiaojiao ; Wang xianlong ; Hai tang ; Zheng qiantao ; Wang xiao ; Zhang hongyong ; Qin guosong ; Cheng jinbo ; Yuan zengqiang ; Wang hongmei ; Zhou qi ; Zhao jianguo
- 英文论文作者：Yao jing ; Cao chunwei ; Huang jiaojiao ; Wang xianlong ; Hai tang ; Zheng qiantao ; Wang xiao ; Zhang hongyong ; Qin guosong ; Cheng jinbo ; Yuan zengqiang ; Wang hongmei ; Zhou qi ; Zhao jianguo ; State Key laboratory of Reproductive Biology ; Chinese Academy of Sciences ; Beijing Farm Animal Research Center ; Institute of Zoology ; Chinese Academy of Sciences ; State Key Laboratory of Brain and Cognitive Sciences ; Institute of Biophysics ; Chinese Academy of Sciences
- 年：2016
- 作者机构：State Key laboratory of Reproductive Biology,Chinese Academy of Sciences;Beijing Farm Animal Research Center,Institute of Zoology,Chinese Academy of Sciences;State Key Laboratory of Brain and Cognitive Sciences,Institute of Biophysics,Chinese Academy of Sciences;
- 英文论文关键词：TALENs ; CRISPR/Cas9 ; pig ; gene targeting ; Parkinson's disease
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：R742.5;R-332
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：7
- 文件大小：2744k
- 原文格式：O
- 会议级别：全国
摘要
Pigs are important for agriculture and study of human diseases.Transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) 9 system(CRISPR/Cas9) show enormous potential for gene engineering in various species,including pigs.Here,we used TALENs and CRISPR/Cas9 system to target single genes(GGTA1,Parkin,DJ-1) and triple genes(Parkin/DJ-1/PINK1) in the porcine genome.The targeting efficiency of TALENs was 2.5%-22%,while the CRISPR/Cas9 system reached an efficiency of up to 100%.DJ-1knockout(KO) piglets were generated by somatic cell nuclear transfer from targeted fibroblasts and Parkin/DJ-1/PINK1 triple targeted pigs were generated by direct embryo injection.These pigs would provide valuable information for studying Parkinson's disease in human.
Pigs are important for agriculture and study of human diseases.Transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) 9 system(CRISPR/Cas9) show enormous potential for gene engineering in various species,including pigs.Here,we used TALENs and CRISPR/Cas9 system to target single genes(GGTA1,Parkin,DJ-1) and triple genes(Parkin/DJ-1/PINK1) in the porcine genome.The targeting efficiency of TALENs was 2.5%-22%,while the CRISPR/Cas9 system reached an efficiency of up to 100%.DJ-1knockout(KO) piglets were generated by somatic cell nuclear transfer from targeted fibroblasts and Parkin/DJ-1/PINK1 triple targeted pigs were generated by direct embryo injection.These pigs would provide valuable information for studying Parkinson's disease in human.
Pigs are important for agriculture and study of human diseases.Transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) 9 system(CRISPR/Cas9) show enormous potential for gene engineering in various species,including pigs.Here,we used TALENs and CRISPR/Cas9 system to target single genes(GGTA1,Parkin,DJ-1) and triple genes(Parkin/DJ-1/PINK1) in the porcine genome.The targeting efficiency of TALENs was 2.5%-22%,while the CRISPR/Cas9 system reached an efficiency of up to 100%.DJ-1knockout(KO) piglets were generated by somatic cell nuclear transfer from targeted fibroblasts and Parkin/DJ-1/PINK1 triple targeted pigs were generated by direct embryo injection.These pigs would provide valuable information for studying Parkinson's disease in human.
引文