家养梅花鹿品种Y染色体遗传多样性和父系遗传结构
详细信息 查看官网全文
- 英文论文题名：Y Chromosomal Genetic Diversityand Patriarchal Genetic Structure of Domestic Sika Deer breeds
- 论文作者：周盼伊 ; 邵元臣 ; 刘华淼 ; 张然然 ; 王磊 ; 邢秀梅
- 英文论文作者：Zhou Panyi ; Shao Yuanchen ; Liu Huamiao ; Zhang Ranran ; Wang Lei ; Xing Xiumei ; State Key Laboratory for Molecular Biology of Special Economic Animals ; Institute of Special Wild Economic Animals and Plants ; Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：中国农业科学院特产研究所特种经济动物分子生物学重点实验室;
- 论文关键词：梅花鹿品种 ; Y染色体 ; AMELY基因 ; 遗传多样性 ; 遗传结构
- 英文论文关键词：sika deer breeds ; Y chromosome ; AMELY gene ; genetic diversity ; genetic structure
- 会议召开时间：2016-06-01
- 会议录名称：“十二五” 鹿科学研究进展
- 语种：中文
- 分类号：S825
- 学会代码：ZXMY
- 学会名称：中国畜牧业协会
- 页数：7
- 文件大小：352k
- 原文格式：O
摘要
为了全面研究家养梅花鹿的遗传多样性及遗传结构,为家养梅花鹿育种工作提供理论依据,利用PCR直接测序法,测定了我国培育的家养梅花鹿品种和品系389头雄性梅花鹿Y染色体AMELY基因部分序列,针对测序得到的序列利用生物信息学软件进行分析,筛选出6个SNPs位点,得到了6种单倍型(H1、H2、H3、H4、H5和H6),结果表明,我国家养梅花鹿Y染色体遗传多样性较低,种群之间没有发生显著的遗传分化。单倍型H1为优势单倍型,在种群中有比较高的频率,其次为单倍型H2,其他单倍型频率比较低。
In order to investigate genetic diversity and genetic structure of domestic sika deer in China comprehensively,and provide theoretical basis for breeding of sika deer.AMELY gene fragments on Y chromosome in 389 individuals from 8 domestic sika deer populations were amplified using PCR method,and the products were sequenced directly.The sequences attained were analyzed through bioinformatics software.Six SNPs were found,and six haplotypes(H1、 H2、 H3、 H4、 H5 and H6) were observed.The domestic populations of Chinese sika deer exhibited a low level in genetic diversity on Y chromosome,and no significant genetic structure difference was found among different populations.H1 was the most dominant haplotype,showed an extraordinary high frequency across all populations.H2 showed the second frequency.Other haplotypes showed a low frequency.
In order to investigate genetic diversity and genetic structure of domestic sika deer in China comprehensively,and provide theoretical basis for breeding of sika deer.AMELY gene fragments on Y chromosome in 389 individuals from 8 domestic sika deer populations were amplified using PCR method,and the products were sequenced directly.The sequences attained were analyzed through bioinformatics software.Six SNPs were found,and six haplotypes(H1、 H2、 H3、 H4、 H5 and H6) were observed.The domestic populations of Chinese sika deer exhibited a low level in genetic diversity on Y chromosome,and no significant genetic structure difference was found among different populations.H1 was the most dominant haplotype,showed an extraordinary high frequency across all populations.H2 showed the second frequency.Other haplotypes showed a low frequency.
引文
[1]方怀龙,李国华,石炎,等.东北三省梅花鹿驯养繁育利用产业的SWOT分析[J].林业实用技术,2014,018(5):47-50.
[2]吴华,胡杰,方盛国,等.中国圈养梅花鹿的遗传多样性和遗传结构[J].动物学杂志,2006,41(4):41-47.
[3]李欢霞.梅花鹿线粒体基因组全序列测定及种群遗传结构分析[D].镇江:江苏科技大学.2012.
[4]徐佳萍,荣敏,杨福合,等.东北梅花鹿线粒体COⅡ基因序列的遗传结构及其系统发育分析[J].特产研究,2011(04):1-4.
[5]吕晓平,魏辅文,李明,等.中国梅花鹿(Cervus nippon)遗传多样性及与日本梅花鹿间的系统关系[J].科学通报,2006,03(3):292-298.
[6]刘海,杨光,魏辅文,等.中国大陆梅花鹿mtDNA控制区序列变异及种群遗传结构分析[J].动物学报,2003,49(1):53-60.
[7]Jobling M A,Tyler-Smith C.The human Y chromosome:an evolutionary marker comes of age.[J].Nature Reviews Genetics,2003,4(8):598-612.
[8]Runfeng Zhang,Ming Cheng,Xiaofeng Li,et al.Y-SNPs haplotype diversity in four Chinese cattle breeds.[J].Animal Biotechnology,2013,24(4):288-292.
[9]Zhang M,Peng Wei-Feng,Yang Cuang-Li,et al.Y chromosome haplotype diversity of domestic sheep(Ovis aries)in northern Eurasia[J].Animal Genetics,2014,45(6):903-907.
[10]Wang Y,Lei X,Wei Y,et al.Y chromosomal haplotype characteristics of domestic sheep(Ovis aries)in China[J].Gene,2015,565:242-245.
[11]Li R,Wang S-,Xu S-,et al.Novel Y-chromosome polymorphisms in Chinese domestic yak[J].Animal Genetics,2014,45(3):449-452.
[12]Wallner B.Identification of genetic variation on the horse y chromosome and the tracing of male founder lineages in modern breeds[J].Plos One,2013,8(4):132-132.
[13]蔡欣.中国黄牛母系和父系起源的分子特征与系统进化研究[D].杨凌:西北农林科技大学.2006.
[14]张云生.中国13个家驴品种mtDNA Cyt b基因及Y染色体微卫星遗传多样性与起源研究[D].杨凌:西北农林科技大学,2009.
[15]凌英会.中国主要地方马群体遗传多样性及系统进化研究[D].北京:中国农业科学院.2010.
[16]张成东.中国骆驼父系和母系起源的分子特征与系统进化研究[D].杨凌:西北农林科技大学.2014.
[2]吴华,胡杰,方盛国,等.中国圈养梅花鹿的遗传多样性和遗传结构[J].动物学杂志,2006,41(4):41-47.
[3]李欢霞.梅花鹿线粒体基因组全序列测定及种群遗传结构分析[D].镇江:江苏科技大学.2012.
[4]徐佳萍,荣敏,杨福合,等.东北梅花鹿线粒体COⅡ基因序列的遗传结构及其系统发育分析[J].特产研究,2011(04):1-4.
[5]吕晓平,魏辅文,李明,等.中国梅花鹿(Cervus nippon)遗传多样性及与日本梅花鹿间的系统关系[J].科学通报,2006,03(3):292-298.
[6]刘海,杨光,魏辅文,等.中国大陆梅花鹿mtDNA控制区序列变异及种群遗传结构分析[J].动物学报,2003,49(1):53-60.
[7]Jobling M A,Tyler-Smith C.The human Y chromosome:an evolutionary marker comes of age.[J].Nature Reviews Genetics,2003,4(8):598-612.
[8]Runfeng Zhang,Ming Cheng,Xiaofeng Li,et al.Y-SNPs haplotype diversity in four Chinese cattle breeds.[J].Animal Biotechnology,2013,24(4):288-292.
[9]Zhang M,Peng Wei-Feng,Yang Cuang-Li,et al.Y chromosome haplotype diversity of domestic sheep(Ovis aries)in northern Eurasia[J].Animal Genetics,2014,45(6):903-907.
[10]Wang Y,Lei X,Wei Y,et al.Y chromosomal haplotype characteristics of domestic sheep(Ovis aries)in China[J].Gene,2015,565:242-245.
[11]Li R,Wang S-,Xu S-,et al.Novel Y-chromosome polymorphisms in Chinese domestic yak[J].Animal Genetics,2014,45(3):449-452.
[12]Wallner B.Identification of genetic variation on the horse y chromosome and the tracing of male founder lineages in modern breeds[J].Plos One,2013,8(4):132-132.
[13]蔡欣.中国黄牛母系和父系起源的分子特征与系统进化研究[D].杨凌:西北农林科技大学.2006.
[14]张云生.中国13个家驴品种mtDNA Cyt b基因及Y染色体微卫星遗传多样性与起源研究[D].杨凌:西北农林科技大学,2009.
[15]凌英会.中国主要地方马群体遗传多样性及系统进化研究[D].北京:中国农业科学院.2010.
[16]张成东.中国骆驼父系和母系起源的分子特征与系统进化研究[D].杨凌:西北农林科技大学.2014.